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Characterizing the normal proteome of human ciliary body.

Goel R, Murthy KR, Srikanth SM, Pinto SM, Bhattacharjee M, Kelkar DS, Madugundu AK, Dey G, Mohan SS, Krishna V, Prasad TsK, Chakravarti S, Harsha H, Pandey A - Clin Proteomics (2013)

Bottom Line: We identified a number of proteins that were previously not described in the ciliary body including importin 5 (IPO5), atlastin-2 (ATL2), B-cell receptor associated protein 29 (BCAP29), basigin (BSG), calpain-1 (CAPN1), copine 6 (CPNE6), fibulin 1 (FBLN1) and galectin 1 (LGALS1).We compared the plasma proteome with the ciliary body proteome and found that the large majority of proteins in the ciliary body were also detectable in the plasma while 896 proteins were unique to the ciliary body.We also classified proteins using pathway enrichment analysis and found most of proteins associated with ubiquitin pathway, EIF2 signaling, glycolysis and gluconeogenesis.

View Article: PubMed Central - HTML - PubMed

Affiliation: Johns Hopkins University School of Medicine, Baltimore 21205, MD, USA. pandey@jhmi.edu.

ABSTRACT

Background: The ciliary body is the circumferential muscular tissue located just behind the iris in the anterior chamber of the eye. It plays a pivotal role in the production of aqueous humor, maintenance of the lens zonules and accommodation by changing the shape of the crystalline lens. The ciliary body is the major target of drugs against glaucoma as its inhibition leads to a drop in intraocular pressure. A molecular study of the ciliary body could provide a better understanding about the pathophysiological processes that occur in glaucoma. Thus far, no large-scale proteomic investigation has been reported for the human ciliary body.

Results: In this study, we have carried out an in-depth LC-MS/MS-based proteomic analysis of normal human ciliary body and have identified 2,815 proteins. We identified a number of proteins that were previously not described in the ciliary body including importin 5 (IPO5), atlastin-2 (ATL2), B-cell receptor associated protein 29 (BCAP29), basigin (BSG), calpain-1 (CAPN1), copine 6 (CPNE6), fibulin 1 (FBLN1) and galectin 1 (LGALS1). We compared the plasma proteome with the ciliary body proteome and found that the large majority of proteins in the ciliary body were also detectable in the plasma while 896 proteins were unique to the ciliary body. We also classified proteins using pathway enrichment analysis and found most of proteins associated with ubiquitin pathway, EIF2 signaling, glycolysis and gluconeogenesis.

Conclusions: More than 95% of the identified proteins have not been previously described in the ciliary body proteome. This is the largest catalogue of proteins reported thus far in the ciliary body that should provide new insights into our understanding of the factors involved in maintaining the secretion of aqueous humor. The identification of these proteins will aid in understanding various eye diseases of the anterior segment such as glaucoma and presbyopia.

No MeSH data available.


Related in: MedlinePlus

Schematic structure of the eye and experimental strategy for proteomic analysis of human ciliary body. Panel A. shows anatomy of the eye with a zoomed in view of the ciliary body. Panel B. depicts the proteomic workflow employed for the study.
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Figure 1: Schematic structure of the eye and experimental strategy for proteomic analysis of human ciliary body. Panel A. shows anatomy of the eye with a zoomed in view of the ciliary body. Panel B. depicts the proteomic workflow employed for the study.

Mentions: The ciliary body, iris and choroid comprise the vascular uveal coat of the eye. The ciliary body forms a ring along the inner wall of the globe and extends from the iris anteriorly to the ora serrata posteriorly as shown in FigureĀ 1A. It is predominantly made up of smooth muscle that is arranged in longitudinal radial and circular fashion. The ciliary body is composed of the ciliary muscle and ciliary processes. Ciliary processes are approximately 70 in number in humans and project inwards as radial ridges [1]. The ciliary body is highly vascular and supplied by the anterior ciliary and long posterior ciliary vessels [2,3]. The ciliary processes consist of a central core of connective tissue stroma which is covered by a double layered epithelium. The inner non-pigmented epithelial layer is in direct contact with the aqueous [4]. It is formed by a layer of columnar cells which contain numerous mitochondria, rough and smooth endoplasmic reticulum which is characteristic of metabolically active cells. The outer-pigmented epithelial cell layer is a layer of cuboidal cells which are abundant in melanosomes that are relatively poor in intracellular organelles. It lies between the non-pigmented epithelial layer and the connective tissue stroma [1].


Characterizing the normal proteome of human ciliary body.

Goel R, Murthy KR, Srikanth SM, Pinto SM, Bhattacharjee M, Kelkar DS, Madugundu AK, Dey G, Mohan SS, Krishna V, Prasad TsK, Chakravarti S, Harsha H, Pandey A - Clin Proteomics (2013)

Schematic structure of the eye and experimental strategy for proteomic analysis of human ciliary body. Panel A. shows anatomy of the eye with a zoomed in view of the ciliary body. Panel B. depicts the proteomic workflow employed for the study.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3750387&req=5

Figure 1: Schematic structure of the eye and experimental strategy for proteomic analysis of human ciliary body. Panel A. shows anatomy of the eye with a zoomed in view of the ciliary body. Panel B. depicts the proteomic workflow employed for the study.
Mentions: The ciliary body, iris and choroid comprise the vascular uveal coat of the eye. The ciliary body forms a ring along the inner wall of the globe and extends from the iris anteriorly to the ora serrata posteriorly as shown in FigureĀ 1A. It is predominantly made up of smooth muscle that is arranged in longitudinal radial and circular fashion. The ciliary body is composed of the ciliary muscle and ciliary processes. Ciliary processes are approximately 70 in number in humans and project inwards as radial ridges [1]. The ciliary body is highly vascular and supplied by the anterior ciliary and long posterior ciliary vessels [2,3]. The ciliary processes consist of a central core of connective tissue stroma which is covered by a double layered epithelium. The inner non-pigmented epithelial layer is in direct contact with the aqueous [4]. It is formed by a layer of columnar cells which contain numerous mitochondria, rough and smooth endoplasmic reticulum which is characteristic of metabolically active cells. The outer-pigmented epithelial cell layer is a layer of cuboidal cells which are abundant in melanosomes that are relatively poor in intracellular organelles. It lies between the non-pigmented epithelial layer and the connective tissue stroma [1].

Bottom Line: We identified a number of proteins that were previously not described in the ciliary body including importin 5 (IPO5), atlastin-2 (ATL2), B-cell receptor associated protein 29 (BCAP29), basigin (BSG), calpain-1 (CAPN1), copine 6 (CPNE6), fibulin 1 (FBLN1) and galectin 1 (LGALS1).We compared the plasma proteome with the ciliary body proteome and found that the large majority of proteins in the ciliary body were also detectable in the plasma while 896 proteins were unique to the ciliary body.We also classified proteins using pathway enrichment analysis and found most of proteins associated with ubiquitin pathway, EIF2 signaling, glycolysis and gluconeogenesis.

View Article: PubMed Central - HTML - PubMed

Affiliation: Johns Hopkins University School of Medicine, Baltimore 21205, MD, USA. pandey@jhmi.edu.

ABSTRACT

Background: The ciliary body is the circumferential muscular tissue located just behind the iris in the anterior chamber of the eye. It plays a pivotal role in the production of aqueous humor, maintenance of the lens zonules and accommodation by changing the shape of the crystalline lens. The ciliary body is the major target of drugs against glaucoma as its inhibition leads to a drop in intraocular pressure. A molecular study of the ciliary body could provide a better understanding about the pathophysiological processes that occur in glaucoma. Thus far, no large-scale proteomic investigation has been reported for the human ciliary body.

Results: In this study, we have carried out an in-depth LC-MS/MS-based proteomic analysis of normal human ciliary body and have identified 2,815 proteins. We identified a number of proteins that were previously not described in the ciliary body including importin 5 (IPO5), atlastin-2 (ATL2), B-cell receptor associated protein 29 (BCAP29), basigin (BSG), calpain-1 (CAPN1), copine 6 (CPNE6), fibulin 1 (FBLN1) and galectin 1 (LGALS1). We compared the plasma proteome with the ciliary body proteome and found that the large majority of proteins in the ciliary body were also detectable in the plasma while 896 proteins were unique to the ciliary body. We also classified proteins using pathway enrichment analysis and found most of proteins associated with ubiquitin pathway, EIF2 signaling, glycolysis and gluconeogenesis.

Conclusions: More than 95% of the identified proteins have not been previously described in the ciliary body proteome. This is the largest catalogue of proteins reported thus far in the ciliary body that should provide new insights into our understanding of the factors involved in maintaining the secretion of aqueous humor. The identification of these proteins will aid in understanding various eye diseases of the anterior segment such as glaucoma and presbyopia.

No MeSH data available.


Related in: MedlinePlus