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A novel approach to fabricate silk nanofibers containing hydroxyapatite nanoparticles using a three-way stopcock connector.

Sheikh FA, Ju HW, Moon BM, Park HJ, Kim JH, Lee OJ, Park CH - Nanoscale Res Lett (2013)

Bottom Line: In this work, we had successfully used a three-way stopcock connector to mix the two different solutions, and very shortly, this solution is ejected out to form nanofibers due to electric fields.Different blend ratios consisting HAp NPs had been electrospun into nanofibers.These characterization techniques revealed that HAp NPs can be easily introduced in silk nanofibers using a stopcock connector, and this method favorably preserves the intact nature of silk fibroin and HAp NPs.

View Article: PubMed Central - HTML - PubMed

Affiliation: Nano-Bio Regenerative Medical Institute, College of Medicine, Hallym University, Chuncheon 200-702, South Korea ; Department of Chemistry, University of Texas-Pan American, Edinburg, Texas 78539, USA.

ABSTRACT
Electrospinning technique is commonly used to produce micro- and/or nanofibers, which utilizes electrical forces to produce polymeric fibers with diameters ranging from several micrometers down to few nanometers. Desirably, electrospun materials provide highly porous structure and appropriate pore size for initial cell attachment and proliferation and thereby enable the exchange of nutrients. Composite nanofibers consisting of silk and hydroxyapatite nanoparticles (HAp) (NPs) had been considered as an excellent choice due to their efficient biocompatibility and bone-mimicking properties. To prepare these nanofiber composites, it requires the use of acidic solutions which have serious consequences on the nature of both silk and HAp NPs. It is ideal to create these nanofibers using aqueous solutions in which the physicochemical nature of both materials can be retained. However, to create those nanofibers is often difficult to obtain because of the fact that aqueous solutions of silk and HAp NPs can precipitate before they can be ejected into fibers during the electrospinning process. In this work, we had successfully used a three-way stopcock connector to mix the two different solutions, and very shortly, this solution is ejected out to form nanofibers due to electric fields. Different blend ratios consisting HAp NPs had been electrospun into nanofibers. The physicochemical aspects of fabricated nanofiber had been characterized by different state of techniques like that of FE-SEM, EDS, TEM, TEM-EDS, TGA, FT-IR, and XRD. These characterization techniques revealed that HAp NPs can be easily introduced in silk nanofibers using a stopcock connector, and this method favorably preserves the intact nature of silk fibroin and HAp NPs. Moreover, nanofibers obtained by this strategy were tested for cell toxicity and cell attachment studies using NIH 3 T3 fibroblasts which indicated non-toxic behavior and good attachment of cells upon incubation in the presence of nanofibers.

No MeSH data available.


Related in: MedlinePlus

Transmission electron microscopy results of silk fibroin nanofibers containing 10% HAp NPs in low (A) and high magnifications (B). The inset in the figure (B) shows the HR-TEM of the encircled area.
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Figure 8: Transmission electron microscopy results of silk fibroin nanofibers containing 10% HAp NPs in low (A) and high magnifications (B). The inset in the figure (B) shows the HR-TEM of the encircled area.

Mentions: Due to the poor resolution of scanning electron microscopy, it can only reveal the surface architect of materials, while internal contents often remain untracked. For this reason, we could not find the exact location of HAp NPs on nanofiber by FE-SEM. Therefore, we used TEM to investigate the location of HAp NPs inside the nanofibers. In this context, Figure 7A,B shows the TEM images in low and high magnifications, obtained after analyzing the pristine nanofibers, which are free of any NPs. In this figure, pristine nanofibers can be seen intact and/or aberrationfree, indicating its pristine nature. Moreover, the morphology of the nanofiber modified with HAp NPs shown in Figure 8B, for low and high magnifications, reveals clear appearance of HAp NPs in nanofibers. As indicated by an arrow (Figure 8A), we can see the separated HAp NPs at the centric position of the nanofiber. Moreover, in Figure 8B, the high magnification image of the marked area near HAp NPs on the nanofiber shows the inset figure indicating the HR-TEM of the encircled area. This inset in the figure shows apparent crystal patterns present to that of the HAp NPs in the nanofibers. Furthermore, these results clearly demonstrate the presence and location of HAp NPs in and around nanofibers.


A novel approach to fabricate silk nanofibers containing hydroxyapatite nanoparticles using a three-way stopcock connector.

Sheikh FA, Ju HW, Moon BM, Park HJ, Kim JH, Lee OJ, Park CH - Nanoscale Res Lett (2013)

Transmission electron microscopy results of silk fibroin nanofibers containing 10% HAp NPs in low (A) and high magnifications (B). The inset in the figure (B) shows the HR-TEM of the encircled area.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3750300&req=5

Figure 8: Transmission electron microscopy results of silk fibroin nanofibers containing 10% HAp NPs in low (A) and high magnifications (B). The inset in the figure (B) shows the HR-TEM of the encircled area.
Mentions: Due to the poor resolution of scanning electron microscopy, it can only reveal the surface architect of materials, while internal contents often remain untracked. For this reason, we could not find the exact location of HAp NPs on nanofiber by FE-SEM. Therefore, we used TEM to investigate the location of HAp NPs inside the nanofibers. In this context, Figure 7A,B shows the TEM images in low and high magnifications, obtained after analyzing the pristine nanofibers, which are free of any NPs. In this figure, pristine nanofibers can be seen intact and/or aberrationfree, indicating its pristine nature. Moreover, the morphology of the nanofiber modified with HAp NPs shown in Figure 8B, for low and high magnifications, reveals clear appearance of HAp NPs in nanofibers. As indicated by an arrow (Figure 8A), we can see the separated HAp NPs at the centric position of the nanofiber. Moreover, in Figure 8B, the high magnification image of the marked area near HAp NPs on the nanofiber shows the inset figure indicating the HR-TEM of the encircled area. This inset in the figure shows apparent crystal patterns present to that of the HAp NPs in the nanofibers. Furthermore, these results clearly demonstrate the presence and location of HAp NPs in and around nanofibers.

Bottom Line: In this work, we had successfully used a three-way stopcock connector to mix the two different solutions, and very shortly, this solution is ejected out to form nanofibers due to electric fields.Different blend ratios consisting HAp NPs had been electrospun into nanofibers.These characterization techniques revealed that HAp NPs can be easily introduced in silk nanofibers using a stopcock connector, and this method favorably preserves the intact nature of silk fibroin and HAp NPs.

View Article: PubMed Central - HTML - PubMed

Affiliation: Nano-Bio Regenerative Medical Institute, College of Medicine, Hallym University, Chuncheon 200-702, South Korea ; Department of Chemistry, University of Texas-Pan American, Edinburg, Texas 78539, USA.

ABSTRACT
Electrospinning technique is commonly used to produce micro- and/or nanofibers, which utilizes electrical forces to produce polymeric fibers with diameters ranging from several micrometers down to few nanometers. Desirably, electrospun materials provide highly porous structure and appropriate pore size for initial cell attachment and proliferation and thereby enable the exchange of nutrients. Composite nanofibers consisting of silk and hydroxyapatite nanoparticles (HAp) (NPs) had been considered as an excellent choice due to their efficient biocompatibility and bone-mimicking properties. To prepare these nanofiber composites, it requires the use of acidic solutions which have serious consequences on the nature of both silk and HAp NPs. It is ideal to create these nanofibers using aqueous solutions in which the physicochemical nature of both materials can be retained. However, to create those nanofibers is often difficult to obtain because of the fact that aqueous solutions of silk and HAp NPs can precipitate before they can be ejected into fibers during the electrospinning process. In this work, we had successfully used a three-way stopcock connector to mix the two different solutions, and very shortly, this solution is ejected out to form nanofibers due to electric fields. Different blend ratios consisting HAp NPs had been electrospun into nanofibers. The physicochemical aspects of fabricated nanofiber had been characterized by different state of techniques like that of FE-SEM, EDS, TEM, TEM-EDS, TGA, FT-IR, and XRD. These characterization techniques revealed that HAp NPs can be easily introduced in silk nanofibers using a stopcock connector, and this method favorably preserves the intact nature of silk fibroin and HAp NPs. Moreover, nanofibers obtained by this strategy were tested for cell toxicity and cell attachment studies using NIH 3 T3 fibroblasts which indicated non-toxic behavior and good attachment of cells upon incubation in the presence of nanofibers.

No MeSH data available.


Related in: MedlinePlus