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A novel approach to fabricate silk nanofibers containing hydroxyapatite nanoparticles using a three-way stopcock connector.

Sheikh FA, Ju HW, Moon BM, Park HJ, Kim JH, Lee OJ, Park CH - Nanoscale Res Lett (2013)

Bottom Line: In this work, we had successfully used a three-way stopcock connector to mix the two different solutions, and very shortly, this solution is ejected out to form nanofibers due to electric fields.Different blend ratios consisting HAp NPs had been electrospun into nanofibers.These characterization techniques revealed that HAp NPs can be easily introduced in silk nanofibers using a stopcock connector, and this method favorably preserves the intact nature of silk fibroin and HAp NPs.

View Article: PubMed Central - HTML - PubMed

Affiliation: Nano-Bio Regenerative Medical Institute, College of Medicine, Hallym University, Chuncheon 200-702, South Korea ; Department of Chemistry, University of Texas-Pan American, Edinburg, Texas 78539, USA.

ABSTRACT
Electrospinning technique is commonly used to produce micro- and/or nanofibers, which utilizes electrical forces to produce polymeric fibers with diameters ranging from several micrometers down to few nanometers. Desirably, electrospun materials provide highly porous structure and appropriate pore size for initial cell attachment and proliferation and thereby enable the exchange of nutrients. Composite nanofibers consisting of silk and hydroxyapatite nanoparticles (HAp) (NPs) had been considered as an excellent choice due to their efficient biocompatibility and bone-mimicking properties. To prepare these nanofiber composites, it requires the use of acidic solutions which have serious consequences on the nature of both silk and HAp NPs. It is ideal to create these nanofibers using aqueous solutions in which the physicochemical nature of both materials can be retained. However, to create those nanofibers is often difficult to obtain because of the fact that aqueous solutions of silk and HAp NPs can precipitate before they can be ejected into fibers during the electrospinning process. In this work, we had successfully used a three-way stopcock connector to mix the two different solutions, and very shortly, this solution is ejected out to form nanofibers due to electric fields. Different blend ratios consisting HAp NPs had been electrospun into nanofibers. The physicochemical aspects of fabricated nanofiber had been characterized by different state of techniques like that of FE-SEM, EDS, TEM, TEM-EDS, TGA, FT-IR, and XRD. These characterization techniques revealed that HAp NPs can be easily introduced in silk nanofibers using a stopcock connector, and this method favorably preserves the intact nature of silk fibroin and HAp NPs. Moreover, nanofibers obtained by this strategy were tested for cell toxicity and cell attachment studies using NIH 3 T3 fibroblasts which indicated non-toxic behavior and good attachment of cells upon incubation in the presence of nanofibers.

No MeSH data available.


Related in: MedlinePlus

Results of the cell attachment after culturing the NIH 3 T3 fibroblasts in the presence of nanofibers for 12 days. For pristine silk fibroin nanofibers (A), silk fibroin nanofibers modified with 10% HAp (B), 30% HAp (C), and 50% HAp (D).
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Figure 16: Results of the cell attachment after culturing the NIH 3 T3 fibroblasts in the presence of nanofibers for 12 days. For pristine silk fibroin nanofibers (A), silk fibroin nanofibers modified with 10% HAp (B), 30% HAp (C), and 50% HAp (D).

Mentions: To find out the cell attachment on nanofibers, the results after culturing the fibroblast for 3 and 12 days is presented in Figures 15 and 16. In case of culturing the cells for 3 days, it can be seen that the cells are properly attaching on nanofiber surfaces. After looking on the cells, it is highly realized that the cells are stress-free and are growing in a healthy manner. Furthermore, the cell attachment results after culturing the cells for 12 days are presented in Figure 15. In this figure, we can see the confluent growth of cells on nanofiber surfaces which further indicates the non-toxic nature of nanocomposites. However, from these figures (i.e., Figures 15 and 16), it can be observed that cell attachment is independent to the presence of HAp in nanofibers.


A novel approach to fabricate silk nanofibers containing hydroxyapatite nanoparticles using a three-way stopcock connector.

Sheikh FA, Ju HW, Moon BM, Park HJ, Kim JH, Lee OJ, Park CH - Nanoscale Res Lett (2013)

Results of the cell attachment after culturing the NIH 3 T3 fibroblasts in the presence of nanofibers for 12 days. For pristine silk fibroin nanofibers (A), silk fibroin nanofibers modified with 10% HAp (B), 30% HAp (C), and 50% HAp (D).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3750300&req=5

Figure 16: Results of the cell attachment after culturing the NIH 3 T3 fibroblasts in the presence of nanofibers for 12 days. For pristine silk fibroin nanofibers (A), silk fibroin nanofibers modified with 10% HAp (B), 30% HAp (C), and 50% HAp (D).
Mentions: To find out the cell attachment on nanofibers, the results after culturing the fibroblast for 3 and 12 days is presented in Figures 15 and 16. In case of culturing the cells for 3 days, it can be seen that the cells are properly attaching on nanofiber surfaces. After looking on the cells, it is highly realized that the cells are stress-free and are growing in a healthy manner. Furthermore, the cell attachment results after culturing the cells for 12 days are presented in Figure 15. In this figure, we can see the confluent growth of cells on nanofiber surfaces which further indicates the non-toxic nature of nanocomposites. However, from these figures (i.e., Figures 15 and 16), it can be observed that cell attachment is independent to the presence of HAp in nanofibers.

Bottom Line: In this work, we had successfully used a three-way stopcock connector to mix the two different solutions, and very shortly, this solution is ejected out to form nanofibers due to electric fields.Different blend ratios consisting HAp NPs had been electrospun into nanofibers.These characterization techniques revealed that HAp NPs can be easily introduced in silk nanofibers using a stopcock connector, and this method favorably preserves the intact nature of silk fibroin and HAp NPs.

View Article: PubMed Central - HTML - PubMed

Affiliation: Nano-Bio Regenerative Medical Institute, College of Medicine, Hallym University, Chuncheon 200-702, South Korea ; Department of Chemistry, University of Texas-Pan American, Edinburg, Texas 78539, USA.

ABSTRACT
Electrospinning technique is commonly used to produce micro- and/or nanofibers, which utilizes electrical forces to produce polymeric fibers with diameters ranging from several micrometers down to few nanometers. Desirably, electrospun materials provide highly porous structure and appropriate pore size for initial cell attachment and proliferation and thereby enable the exchange of nutrients. Composite nanofibers consisting of silk and hydroxyapatite nanoparticles (HAp) (NPs) had been considered as an excellent choice due to their efficient biocompatibility and bone-mimicking properties. To prepare these nanofiber composites, it requires the use of acidic solutions which have serious consequences on the nature of both silk and HAp NPs. It is ideal to create these nanofibers using aqueous solutions in which the physicochemical nature of both materials can be retained. However, to create those nanofibers is often difficult to obtain because of the fact that aqueous solutions of silk and HAp NPs can precipitate before they can be ejected into fibers during the electrospinning process. In this work, we had successfully used a three-way stopcock connector to mix the two different solutions, and very shortly, this solution is ejected out to form nanofibers due to electric fields. Different blend ratios consisting HAp NPs had been electrospun into nanofibers. The physicochemical aspects of fabricated nanofiber had been characterized by different state of techniques like that of FE-SEM, EDS, TEM, TEM-EDS, TGA, FT-IR, and XRD. These characterization techniques revealed that HAp NPs can be easily introduced in silk nanofibers using a stopcock connector, and this method favorably preserves the intact nature of silk fibroin and HAp NPs. Moreover, nanofibers obtained by this strategy were tested for cell toxicity and cell attachment studies using NIH 3 T3 fibroblasts which indicated non-toxic behavior and good attachment of cells upon incubation in the presence of nanofibers.

No MeSH data available.


Related in: MedlinePlus