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A novel approach to fabricate silk nanofibers containing hydroxyapatite nanoparticles using a three-way stopcock connector.

Sheikh FA, Ju HW, Moon BM, Park HJ, Kim JH, Lee OJ, Park CH - Nanoscale Res Lett (2013)

Bottom Line: In this work, we had successfully used a three-way stopcock connector to mix the two different solutions, and very shortly, this solution is ejected out to form nanofibers due to electric fields.Different blend ratios consisting HAp NPs had been electrospun into nanofibers.These characterization techniques revealed that HAp NPs can be easily introduced in silk nanofibers using a stopcock connector, and this method favorably preserves the intact nature of silk fibroin and HAp NPs.

View Article: PubMed Central - HTML - PubMed

Affiliation: Nano-Bio Regenerative Medical Institute, College of Medicine, Hallym University, Chuncheon 200-702, South Korea ; Department of Chemistry, University of Texas-Pan American, Edinburg, Texas 78539, USA.

ABSTRACT
Electrospinning technique is commonly used to produce micro- and/or nanofibers, which utilizes electrical forces to produce polymeric fibers with diameters ranging from several micrometers down to few nanometers. Desirably, electrospun materials provide highly porous structure and appropriate pore size for initial cell attachment and proliferation and thereby enable the exchange of nutrients. Composite nanofibers consisting of silk and hydroxyapatite nanoparticles (HAp) (NPs) had been considered as an excellent choice due to their efficient biocompatibility and bone-mimicking properties. To prepare these nanofiber composites, it requires the use of acidic solutions which have serious consequences on the nature of both silk and HAp NPs. It is ideal to create these nanofibers using aqueous solutions in which the physicochemical nature of both materials can be retained. However, to create those nanofibers is often difficult to obtain because of the fact that aqueous solutions of silk and HAp NPs can precipitate before they can be ejected into fibers during the electrospinning process. In this work, we had successfully used a three-way stopcock connector to mix the two different solutions, and very shortly, this solution is ejected out to form nanofibers due to electric fields. Different blend ratios consisting HAp NPs had been electrospun into nanofibers. The physicochemical aspects of fabricated nanofiber had been characterized by different state of techniques like that of FE-SEM, EDS, TEM, TEM-EDS, TGA, FT-IR, and XRD. These characterization techniques revealed that HAp NPs can be easily introduced in silk nanofibers using a stopcock connector, and this method favorably preserves the intact nature of silk fibroin and HAp NPs. Moreover, nanofibers obtained by this strategy were tested for cell toxicity and cell attachment studies using NIH 3 T3 fibroblasts which indicated non-toxic behavior and good attachment of cells upon incubation in the presence of nanofibers.

No MeSH data available.


Related in: MedlinePlus

The TGA results for the obtained nanofibers. The inset in the figure shows the derivative of weight loss for the obtained nanofibers.
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Figure 13: The TGA results for the obtained nanofibers. The inset in the figure shows the derivative of weight loss for the obtained nanofibers.

Mentions: Figure 13 shows the results obtained after thermogravimetric analyses (TGA) of pristine and nanofibers modified HAp NPs. It was expected that the introduction of HAp NPs on the nanofibers would result in the improvement in thermal and crystalline properties of the nanofibers. After analyzing the data, it was observed that all the nanofiber samples showed initial weight loss of about 4% to 6% until 100°C, which is due to the removal of residual moisture. The onset temperatures of pristine nanofiber was calculated to be 269°C, and the nanofibers modified with HAp NPs represented higher onset temperatures of 273°C, 275°C, and 276°C. This high onset temperatures in case of nanofibers modified with HAp can be corroborated due to the β-sheet crystalline structures and covalent bonding of silk fibroin with HAp NPs, which result to the increase in the onset temperatures. The inset in the figure of the graph (Figure 13) represents the derivative of weight loss for nanofibers. As indicated in the inset in the figure, the first step degradation occurring in all nanofiber combinations can be clearly seen at 293°C which can be assigned due to the degradation of silk fibroins. Moreover, the nanofibers modified with HAp NPs show the second step degradation point at 409°C, which sharpens as the concentration of HAp is increased in nanofibers. Interestingly, it further clarifies that the molecular orientation and/or the crystallinity of silk fibroin can be improved by the incorporation of HAp NPs at higher amounts. At 693°C, the weight residues remaining for pristine nanofibers were calculated to be 9%, and the nanofibers modified by HAp NPs showed the increased residual weight remaining of 11%, 23%, and 27%. This increase in residual weights is due to the reason that HAp NPs had high thermal stability than the pure silk fibroin which probably helped the other modified counterparts to gain more residual weights of that of the pristine one.


A novel approach to fabricate silk nanofibers containing hydroxyapatite nanoparticles using a three-way stopcock connector.

Sheikh FA, Ju HW, Moon BM, Park HJ, Kim JH, Lee OJ, Park CH - Nanoscale Res Lett (2013)

The TGA results for the obtained nanofibers. The inset in the figure shows the derivative of weight loss for the obtained nanofibers.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3750300&req=5

Figure 13: The TGA results for the obtained nanofibers. The inset in the figure shows the derivative of weight loss for the obtained nanofibers.
Mentions: Figure 13 shows the results obtained after thermogravimetric analyses (TGA) of pristine and nanofibers modified HAp NPs. It was expected that the introduction of HAp NPs on the nanofibers would result in the improvement in thermal and crystalline properties of the nanofibers. After analyzing the data, it was observed that all the nanofiber samples showed initial weight loss of about 4% to 6% until 100°C, which is due to the removal of residual moisture. The onset temperatures of pristine nanofiber was calculated to be 269°C, and the nanofibers modified with HAp NPs represented higher onset temperatures of 273°C, 275°C, and 276°C. This high onset temperatures in case of nanofibers modified with HAp can be corroborated due to the β-sheet crystalline structures and covalent bonding of silk fibroin with HAp NPs, which result to the increase in the onset temperatures. The inset in the figure of the graph (Figure 13) represents the derivative of weight loss for nanofibers. As indicated in the inset in the figure, the first step degradation occurring in all nanofiber combinations can be clearly seen at 293°C which can be assigned due to the degradation of silk fibroins. Moreover, the nanofibers modified with HAp NPs show the second step degradation point at 409°C, which sharpens as the concentration of HAp is increased in nanofibers. Interestingly, it further clarifies that the molecular orientation and/or the crystallinity of silk fibroin can be improved by the incorporation of HAp NPs at higher amounts. At 693°C, the weight residues remaining for pristine nanofibers were calculated to be 9%, and the nanofibers modified by HAp NPs showed the increased residual weight remaining of 11%, 23%, and 27%. This increase in residual weights is due to the reason that HAp NPs had high thermal stability than the pure silk fibroin which probably helped the other modified counterparts to gain more residual weights of that of the pristine one.

Bottom Line: In this work, we had successfully used a three-way stopcock connector to mix the two different solutions, and very shortly, this solution is ejected out to form nanofibers due to electric fields.Different blend ratios consisting HAp NPs had been electrospun into nanofibers.These characterization techniques revealed that HAp NPs can be easily introduced in silk nanofibers using a stopcock connector, and this method favorably preserves the intact nature of silk fibroin and HAp NPs.

View Article: PubMed Central - HTML - PubMed

Affiliation: Nano-Bio Regenerative Medical Institute, College of Medicine, Hallym University, Chuncheon 200-702, South Korea ; Department of Chemistry, University of Texas-Pan American, Edinburg, Texas 78539, USA.

ABSTRACT
Electrospinning technique is commonly used to produce micro- and/or nanofibers, which utilizes electrical forces to produce polymeric fibers with diameters ranging from several micrometers down to few nanometers. Desirably, electrospun materials provide highly porous structure and appropriate pore size for initial cell attachment and proliferation and thereby enable the exchange of nutrients. Composite nanofibers consisting of silk and hydroxyapatite nanoparticles (HAp) (NPs) had been considered as an excellent choice due to their efficient biocompatibility and bone-mimicking properties. To prepare these nanofiber composites, it requires the use of acidic solutions which have serious consequences on the nature of both silk and HAp NPs. It is ideal to create these nanofibers using aqueous solutions in which the physicochemical nature of both materials can be retained. However, to create those nanofibers is often difficult to obtain because of the fact that aqueous solutions of silk and HAp NPs can precipitate before they can be ejected into fibers during the electrospinning process. In this work, we had successfully used a three-way stopcock connector to mix the two different solutions, and very shortly, this solution is ejected out to form nanofibers due to electric fields. Different blend ratios consisting HAp NPs had been electrospun into nanofibers. The physicochemical aspects of fabricated nanofiber had been characterized by different state of techniques like that of FE-SEM, EDS, TEM, TEM-EDS, TGA, FT-IR, and XRD. These characterization techniques revealed that HAp NPs can be easily introduced in silk nanofibers using a stopcock connector, and this method favorably preserves the intact nature of silk fibroin and HAp NPs. Moreover, nanofibers obtained by this strategy were tested for cell toxicity and cell attachment studies using NIH 3 T3 fibroblasts which indicated non-toxic behavior and good attachment of cells upon incubation in the presence of nanofibers.

No MeSH data available.


Related in: MedlinePlus