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Diversity in bacterium-host interactions within the species Helicobacter heilmannii sensu stricto.

Joosten M, Blaecher C, Flahou B, Ducatelle R, Haesebrouck F, Smet A - Vet. Res. (2013)

Bottom Line: A reduced antral expression of H+/K+ ATPase was seen in the stomach after infection with 3 highly colonizing strains and 2 highly colonizing strains caused an increased gastrin expression in the fundus.In none of the H. heilmannii s.s.-infected groups, IFN-γ expression was up-regulated.This study demonstrates diversity in bacterium-host interactions within the species H. heilmannii s.s. and that the pathogenesis of gastric infections with this microorganism is not identical to that of an H. pylori infection.

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ABSTRACT
Helicobacter (H.) heilmannii sensu stricto (s.s.) is a zoonotic bacterium that naturally colonizes the stomach of dogs and cats. In humans, this microorganism has been associated with gastritis, peptic ulcer disease and mucosa associated lymphoid tissue (MALT) lymphoma. Little information is available about the pathogenesis of H. heilmannii s.s. infections in humans and it is unknown whether differences in virulence exist within this species. Therefore, a Mongolian gerbil model was used to study bacterium-host interactions of 9 H. heilmannii s.s. strains. The colonization ability of the strains, the intensity of gastritis and gene expression of various inflammatory cytokines in the stomach were determined at 9 weeks after experimental infection. The induction of an antrum-dominant chronic active gastritis with formation of lymphocytic aggregates was shown for 7 strains. High-level antral colonization was seen for 4 strains, while colonization of 4 other strains was more restricted and one strain was not detected in the stomach at 9 weeks post infection. All strains inducing a chronic active gastritis caused an up-regulation of the pro-inflammatory cytokine IL-1β in the antrum. A reduced antral expression of H+/K+ ATPase was seen in the stomach after infection with 3 highly colonizing strains and 2 highly colonizing strains caused an increased gastrin expression in the fundus. In none of the H. heilmannii s.s.-infected groups, IFN-γ expression was up-regulated. This study demonstrates diversity in bacterium-host interactions within the species H. heilmannii s.s. and that the pathogenesis of gastric infections with this microorganism is not identical to that of an H. pylori infection.

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mRNA expression levels of cytokines IL-1β and IFN-γ in the antrum of the stomach. Cytokine mRNA expression levels in the fundus and antrum of the stomach were examined by quantitative RT-PCR. Expression levels of IL-1β (a) and IFN-γ (b) in the antrum are shown. Data are presented as the fold change in gene expression normalized to 3 reference genes and relative to the negative control group which is considered as 1. Data are shown as means + standard deviation. Significant differences in expression level between inoculated groups and negative control group are indicated by * p < 0.05 (ANOVA).
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Figure 4: mRNA expression levels of cytokines IL-1β and IFN-γ in the antrum of the stomach. Cytokine mRNA expression levels in the fundus and antrum of the stomach were examined by quantitative RT-PCR. Expression levels of IL-1β (a) and IFN-γ (b) in the antrum are shown. Data are presented as the fold change in gene expression normalized to 3 reference genes and relative to the negative control group which is considered as 1. Data are shown as means + standard deviation. Significant differences in expression level between inoculated groups and negative control group are indicated by * p < 0.05 (ANOVA).

Mentions: The local host immune response towards H. heilmannii s.s. infection was characterized by measuring the mRNA expression level of IFN-γ, IL-1β, IL-5, IL-6, IL-10, IL-12p40, IL-17 and TNF-α in the stomach of the gerbils. Results are shown in Table 2 and in Figure 4.


Diversity in bacterium-host interactions within the species Helicobacter heilmannii sensu stricto.

Joosten M, Blaecher C, Flahou B, Ducatelle R, Haesebrouck F, Smet A - Vet. Res. (2013)

mRNA expression levels of cytokines IL-1β and IFN-γ in the antrum of the stomach. Cytokine mRNA expression levels in the fundus and antrum of the stomach were examined by quantitative RT-PCR. Expression levels of IL-1β (a) and IFN-γ (b) in the antrum are shown. Data are presented as the fold change in gene expression normalized to 3 reference genes and relative to the negative control group which is considered as 1. Data are shown as means + standard deviation. Significant differences in expression level between inoculated groups and negative control group are indicated by * p < 0.05 (ANOVA).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3750284&req=5

Figure 4: mRNA expression levels of cytokines IL-1β and IFN-γ in the antrum of the stomach. Cytokine mRNA expression levels in the fundus and antrum of the stomach were examined by quantitative RT-PCR. Expression levels of IL-1β (a) and IFN-γ (b) in the antrum are shown. Data are presented as the fold change in gene expression normalized to 3 reference genes and relative to the negative control group which is considered as 1. Data are shown as means + standard deviation. Significant differences in expression level between inoculated groups and negative control group are indicated by * p < 0.05 (ANOVA).
Mentions: The local host immune response towards H. heilmannii s.s. infection was characterized by measuring the mRNA expression level of IFN-γ, IL-1β, IL-5, IL-6, IL-10, IL-12p40, IL-17 and TNF-α in the stomach of the gerbils. Results are shown in Table 2 and in Figure 4.

Bottom Line: A reduced antral expression of H+/K+ ATPase was seen in the stomach after infection with 3 highly colonizing strains and 2 highly colonizing strains caused an increased gastrin expression in the fundus.In none of the H. heilmannii s.s.-infected groups, IFN-γ expression was up-regulated.This study demonstrates diversity in bacterium-host interactions within the species H. heilmannii s.s. and that the pathogenesis of gastric infections with this microorganism is not identical to that of an H. pylori infection.

View Article: PubMed Central - HTML - PubMed

ABSTRACT
Helicobacter (H.) heilmannii sensu stricto (s.s.) is a zoonotic bacterium that naturally colonizes the stomach of dogs and cats. In humans, this microorganism has been associated with gastritis, peptic ulcer disease and mucosa associated lymphoid tissue (MALT) lymphoma. Little information is available about the pathogenesis of H. heilmannii s.s. infections in humans and it is unknown whether differences in virulence exist within this species. Therefore, a Mongolian gerbil model was used to study bacterium-host interactions of 9 H. heilmannii s.s. strains. The colonization ability of the strains, the intensity of gastritis and gene expression of various inflammatory cytokines in the stomach were determined at 9 weeks after experimental infection. The induction of an antrum-dominant chronic active gastritis with formation of lymphocytic aggregates was shown for 7 strains. High-level antral colonization was seen for 4 strains, while colonization of 4 other strains was more restricted and one strain was not detected in the stomach at 9 weeks post infection. All strains inducing a chronic active gastritis caused an up-regulation of the pro-inflammatory cytokine IL-1β in the antrum. A reduced antral expression of H+/K+ ATPase was seen in the stomach after infection with 3 highly colonizing strains and 2 highly colonizing strains caused an increased gastrin expression in the fundus. In none of the H. heilmannii s.s.-infected groups, IFN-γ expression was up-regulated. This study demonstrates diversity in bacterium-host interactions within the species H. heilmannii s.s. and that the pathogenesis of gastric infections with this microorganism is not identical to that of an H. pylori infection.

Show MeSH
Related in: MedlinePlus