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Impact of the LDL subfraction phenotype on Lp-PLA2 distribution, LDL modification and HDL composition in type 2 diabetes.

Sánchez-Quesada JL, Vinagre I, De Juan-Franco E, Sánchez-Hernández J, Bonet-Marques R, Blanco-Vaca F, Ordóñez-Llanos J, Pérez A - Cardiovasc Diabetol (2013)

Bottom Line: Several forms of modified LDL, HDL composition and the activity and distribution of lipoprotein-associated phospholipase A2 (Lp-PLA2) were analyzed.Total Lp-PLA2 activity was higher in phenotype B than in phenotype A or in control subjects.The distribution of Lp-PLA2 between HDL and apoB-containing lipoproteins differed in patients with phenotype A and phenotype B, with higher activity associated to apoB-containing lipoproteins in the latter.

View Article: PubMed Central - HTML - PubMed

Affiliation: Biomedical Research Institute IIB Sant Pau, Cardiovascular Biochemistry Group, C/ Antoni Maria Claret, 167, 08025 Barcelona, Spain. jsanchezq@santpau.cat

ABSTRACT

Background: Qualitative alterations of lipoproteins underlie the high incidence of atherosclerosis in diabetes. The objective of this study was to assess the impact of low-density lipoprotein (LDL) subfraction phenotype on the qualitative characteristics of LDL and high-density lipoprotein (HDL) in patients with type 2 diabetes.

Methods: One hundred twenty two patients with type 2 diabetes in poor glycemic control and 54 healthy subjects were included in the study. Patients were classified according to their LDL subfraction phenotype. Seventy-seven patients presented phenotype A whereas 45 had phenotype B. All control subjects showed phenotype A. Several forms of modified LDL, HDL composition and the activity and distribution of lipoprotein-associated phospholipase A2 (Lp-PLA2) were analyzed.

Results: Oxidized LDL, glycated LDL and electronegative LDL were increased in both groups of patients compared with the control group. Patients with phenotype B had increased oxidized LDL and glycated LDL concentration than patients with phenotype A. HDL composition was abnormal in patients with diabetes, being these abnormalities more marked in patients with phenotype B. Total Lp-PLA2 activity was higher in phenotype B than in phenotype A or in control subjects. The distribution of Lp-PLA2 between HDL and apoB-containing lipoproteins differed in patients with phenotype A and phenotype B, with higher activity associated to apoB-containing lipoproteins in the latter.

Conclusions: The presence of LDL subfraction phenotype B is associated with increased oxidized LDL, glycated LDL and Lp-PLA2 activity associated to apoB-containing lipoproteins, as well as with abnormal HDL composition.

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Related in: MedlinePlus

Lp-PLA2 activity. Enzymatic activity (A) and relative distribution between HDL and apoB-containing lipoproteins (B) of Lp-PLA2 in control subjects and patients with type 2 diabetes classified according their LDL subfraction phenotype. * indicates P < 0.05.
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Figure 1: Lp-PLA2 activity. Enzymatic activity (A) and relative distribution between HDL and apoB-containing lipoproteins (B) of Lp-PLA2 in control subjects and patients with type 2 diabetes classified according their LDL subfraction phenotype. * indicates P < 0.05.

Mentions: Total and apoB-associated Lp-PLA2 activity was higher in patients with phenotype B than in control subjects and in patients with phenotype A, and was similar in the two latter groups (Table 2, Figure 1A). No statistical difference was observed in the HDL-associated Lp-PLA2 activity. Consequently, the distribution of Lp-PLA2 between HDL and apoB-containing lipoproteins (Table 2, Figure 1B) was different between patients with phenotype A and those with phenotype B. The latter had lower proportion of Lp-PLA2 in HDL and higher proportion in apoB-containing lipoproteins than in patients with phenotype A.


Impact of the LDL subfraction phenotype on Lp-PLA2 distribution, LDL modification and HDL composition in type 2 diabetes.

Sánchez-Quesada JL, Vinagre I, De Juan-Franco E, Sánchez-Hernández J, Bonet-Marques R, Blanco-Vaca F, Ordóñez-Llanos J, Pérez A - Cardiovasc Diabetol (2013)

Lp-PLA2 activity. Enzymatic activity (A) and relative distribution between HDL and apoB-containing lipoproteins (B) of Lp-PLA2 in control subjects and patients with type 2 diabetes classified according their LDL subfraction phenotype. * indicates P < 0.05.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3750253&req=5

Figure 1: Lp-PLA2 activity. Enzymatic activity (A) and relative distribution between HDL and apoB-containing lipoproteins (B) of Lp-PLA2 in control subjects and patients with type 2 diabetes classified according their LDL subfraction phenotype. * indicates P < 0.05.
Mentions: Total and apoB-associated Lp-PLA2 activity was higher in patients with phenotype B than in control subjects and in patients with phenotype A, and was similar in the two latter groups (Table 2, Figure 1A). No statistical difference was observed in the HDL-associated Lp-PLA2 activity. Consequently, the distribution of Lp-PLA2 between HDL and apoB-containing lipoproteins (Table 2, Figure 1B) was different between patients with phenotype A and those with phenotype B. The latter had lower proportion of Lp-PLA2 in HDL and higher proportion in apoB-containing lipoproteins than in patients with phenotype A.

Bottom Line: Several forms of modified LDL, HDL composition and the activity and distribution of lipoprotein-associated phospholipase A2 (Lp-PLA2) were analyzed.Total Lp-PLA2 activity was higher in phenotype B than in phenotype A or in control subjects.The distribution of Lp-PLA2 between HDL and apoB-containing lipoproteins differed in patients with phenotype A and phenotype B, with higher activity associated to apoB-containing lipoproteins in the latter.

View Article: PubMed Central - HTML - PubMed

Affiliation: Biomedical Research Institute IIB Sant Pau, Cardiovascular Biochemistry Group, C/ Antoni Maria Claret, 167, 08025 Barcelona, Spain. jsanchezq@santpau.cat

ABSTRACT

Background: Qualitative alterations of lipoproteins underlie the high incidence of atherosclerosis in diabetes. The objective of this study was to assess the impact of low-density lipoprotein (LDL) subfraction phenotype on the qualitative characteristics of LDL and high-density lipoprotein (HDL) in patients with type 2 diabetes.

Methods: One hundred twenty two patients with type 2 diabetes in poor glycemic control and 54 healthy subjects were included in the study. Patients were classified according to their LDL subfraction phenotype. Seventy-seven patients presented phenotype A whereas 45 had phenotype B. All control subjects showed phenotype A. Several forms of modified LDL, HDL composition and the activity and distribution of lipoprotein-associated phospholipase A2 (Lp-PLA2) were analyzed.

Results: Oxidized LDL, glycated LDL and electronegative LDL were increased in both groups of patients compared with the control group. Patients with phenotype B had increased oxidized LDL and glycated LDL concentration than patients with phenotype A. HDL composition was abnormal in patients with diabetes, being these abnormalities more marked in patients with phenotype B. Total Lp-PLA2 activity was higher in phenotype B than in phenotype A or in control subjects. The distribution of Lp-PLA2 between HDL and apoB-containing lipoproteins differed in patients with phenotype A and phenotype B, with higher activity associated to apoB-containing lipoproteins in the latter.

Conclusions: The presence of LDL subfraction phenotype B is associated with increased oxidized LDL, glycated LDL and Lp-PLA2 activity associated to apoB-containing lipoproteins, as well as with abnormal HDL composition.

Show MeSH
Related in: MedlinePlus