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Heat shock protein 27 is spatially distributed in the human placenta and decreased during labor.

Abdulsid A, Fletcher A, Lyall F - PLoS ONE (2013)

Bottom Line: For labor, there was also less HSP 27 protein in the inner region compared with both the middle (p<0.02) and outer region (p<0.01).Similar to HSP 27 protein, there was less HSP 27 mRNA in the labor group in both the inner region (p<0.05) and middle region (p<0.02) compared to non-labor.The results have important implications for how data obtained from studies in the placenta can be influenced by sampling methods.

View Article: PubMed Central - PubMed

Affiliation: University of Glasgow School of Medicine, Institute of Medical Genetics, Yorkhill Hospital, Glasgow, United Kingdom.

ABSTRACT
Placental oxidative stress is a feature of human labor. Heat shock proteins (HSPs) play a key role in cellular stress. We hypothesized that placental expression of the small HSP 27 would be altered during labor and expression would vary in different regions of the placenta. Six women in labor who delivered vaginally and 6 women not in labor, who were delivered by Cesarean section, were recruited. Four equally spaced pieces were sampled from the inner, middle and outer regions of each placenta (total 12 samples per placenta). HSP 27 expression was investigated by Western blot analysis and RT-PCR. For non-labor, there was less HSP 27 protein in the inner placenta region compared with both the middle region (p<0.05) and outer region (p<0.05). For labor, there was also less HSP 27 protein in the inner region compared with both the middle (p<0.02) and outer region (p<0.01). When the 3 regions of the placenta were compared for non-labor versus labor there was less HSP 27 in the labor group at both the inner (p<0.05) and middle regions (p<0.005) compared to non-labor. Similar to HSP 27 protein, there was less HSP 27 mRNA in the labor group in both the inner region (p<0.05) and middle region (p<0.02) compared to non-labor. This study suggests that placental HSP 27 may play a role in labor and is spatially controlled. The results have important implications for how data obtained from studies in the placenta can be influenced by sampling methods.

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Related in: MedlinePlus

Western blot analysis of HSP 27 expression in non-labor versus labor measured at three distances from the cord insertion point of the placenta: inner zone (top panel), middle zones (middle panel) and outer zone (bottom panel).N = 6 in each group of patients.
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pone-0071127-g004: Western blot analysis of HSP 27 expression in non-labor versus labor measured at three distances from the cord insertion point of the placenta: inner zone (top panel), middle zones (middle panel) and outer zone (bottom panel).N = 6 in each group of patients.

Mentions: The next set of experiments was designed to test whether there was a difference in HSP 27 expression between labor and non-labor groups for each of the three sites. Figure 4 shows one representative blot of non-labor versus labor for each of the three different areas of the placenta (upper panel; inner, middle panel; middle and lower panel; outer). Each of the 3 blots were performed on separate days so only labor with non-labor can be compared for this. Figure 5 shows the combined analysis for each of the three groups. Overall there was a significant difference between the 3 areas of the placenta when comparing each non-labor group and labor group in each zone (ANOVA p<0.05). When individual zones were compared there was less HSP 27 in the labor group at both the inner (p<0.05) and middle zones (p<0.005).


Heat shock protein 27 is spatially distributed in the human placenta and decreased during labor.

Abdulsid A, Fletcher A, Lyall F - PLoS ONE (2013)

Western blot analysis of HSP 27 expression in non-labor versus labor measured at three distances from the cord insertion point of the placenta: inner zone (top panel), middle zones (middle panel) and outer zone (bottom panel).N = 6 in each group of patients.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3750034&req=5

pone-0071127-g004: Western blot analysis of HSP 27 expression in non-labor versus labor measured at three distances from the cord insertion point of the placenta: inner zone (top panel), middle zones (middle panel) and outer zone (bottom panel).N = 6 in each group of patients.
Mentions: The next set of experiments was designed to test whether there was a difference in HSP 27 expression between labor and non-labor groups for each of the three sites. Figure 4 shows one representative blot of non-labor versus labor for each of the three different areas of the placenta (upper panel; inner, middle panel; middle and lower panel; outer). Each of the 3 blots were performed on separate days so only labor with non-labor can be compared for this. Figure 5 shows the combined analysis for each of the three groups. Overall there was a significant difference between the 3 areas of the placenta when comparing each non-labor group and labor group in each zone (ANOVA p<0.05). When individual zones were compared there was less HSP 27 in the labor group at both the inner (p<0.05) and middle zones (p<0.005).

Bottom Line: For labor, there was also less HSP 27 protein in the inner region compared with both the middle (p<0.02) and outer region (p<0.01).Similar to HSP 27 protein, there was less HSP 27 mRNA in the labor group in both the inner region (p<0.05) and middle region (p<0.02) compared to non-labor.The results have important implications for how data obtained from studies in the placenta can be influenced by sampling methods.

View Article: PubMed Central - PubMed

Affiliation: University of Glasgow School of Medicine, Institute of Medical Genetics, Yorkhill Hospital, Glasgow, United Kingdom.

ABSTRACT
Placental oxidative stress is a feature of human labor. Heat shock proteins (HSPs) play a key role in cellular stress. We hypothesized that placental expression of the small HSP 27 would be altered during labor and expression would vary in different regions of the placenta. Six women in labor who delivered vaginally and 6 women not in labor, who were delivered by Cesarean section, were recruited. Four equally spaced pieces were sampled from the inner, middle and outer regions of each placenta (total 12 samples per placenta). HSP 27 expression was investigated by Western blot analysis and RT-PCR. For non-labor, there was less HSP 27 protein in the inner placenta region compared with both the middle region (p<0.05) and outer region (p<0.05). For labor, there was also less HSP 27 protein in the inner region compared with both the middle (p<0.02) and outer region (p<0.01). When the 3 regions of the placenta were compared for non-labor versus labor there was less HSP 27 in the labor group at both the inner (p<0.05) and middle regions (p<0.005) compared to non-labor. Similar to HSP 27 protein, there was less HSP 27 mRNA in the labor group in both the inner region (p<0.05) and middle region (p<0.02) compared to non-labor. This study suggests that placental HSP 27 may play a role in labor and is spatially controlled. The results have important implications for how data obtained from studies in the placenta can be influenced by sampling methods.

Show MeSH
Related in: MedlinePlus