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Heat shock protein 27 is spatially distributed in the human placenta and decreased during labor.

Abdulsid A, Fletcher A, Lyall F - PLoS ONE (2013)

Bottom Line: For labor, there was also less HSP 27 protein in the inner region compared with both the middle (p<0.02) and outer region (p<0.01).Similar to HSP 27 protein, there was less HSP 27 mRNA in the labor group in both the inner region (p<0.05) and middle region (p<0.02) compared to non-labor.The results have important implications for how data obtained from studies in the placenta can be influenced by sampling methods.

View Article: PubMed Central - PubMed

Affiliation: University of Glasgow School of Medicine, Institute of Medical Genetics, Yorkhill Hospital, Glasgow, United Kingdom.

ABSTRACT
Placental oxidative stress is a feature of human labor. Heat shock proteins (HSPs) play a key role in cellular stress. We hypothesized that placental expression of the small HSP 27 would be altered during labor and expression would vary in different regions of the placenta. Six women in labor who delivered vaginally and 6 women not in labor, who were delivered by Cesarean section, were recruited. Four equally spaced pieces were sampled from the inner, middle and outer regions of each placenta (total 12 samples per placenta). HSP 27 expression was investigated by Western blot analysis and RT-PCR. For non-labor, there was less HSP 27 protein in the inner placenta region compared with both the middle region (p<0.05) and outer region (p<0.05). For labor, there was also less HSP 27 protein in the inner region compared with both the middle (p<0.02) and outer region (p<0.01). When the 3 regions of the placenta were compared for non-labor versus labor there was less HSP 27 in the labor group at both the inner (p<0.05) and middle regions (p<0.005) compared to non-labor. Similar to HSP 27 protein, there was less HSP 27 mRNA in the labor group in both the inner region (p<0.05) and middle region (p<0.02) compared to non-labor. This study suggests that placental HSP 27 may play a role in labor and is spatially controlled. The results have important implications for how data obtained from studies in the placenta can be influenced by sampling methods.

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Median optical densities for HSP 27 expression in three different placenta zones for all patients.The upper panel shows non-labor (n = 6 patients) and the lower panel shows labor (n = 6 patients). Samples were compared with the Mann Whitney U test. Values are shown as median and median absolute deviation. n..s., non-significant.
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pone-0071127-g003: Median optical densities for HSP 27 expression in three different placenta zones for all patients.The upper panel shows non-labor (n = 6 patients) and the lower panel shows labor (n = 6 patients). Samples were compared with the Mann Whitney U test. Values are shown as median and median absolute deviation. n..s., non-significant.

Mentions: The first set of experiments was designed to test whether there was a difference in HSP 27 expression within an individual placenta in either labor or non-labor. Figure 2 shows HSP 27 expression in the four zones of the inner, middle and outer area sampled from the cord insertion point. The upper panel shows a placenta obtained from a non-laboring caesarean section delivery. The bottom panel shows a placenta obtained from a women who was in labor and delivered vaginally. Figure 3 shows the combined analysis of all the placentae for either non-labor (upper graph) or labor (lower graph). Overall there was a significant difference between the 3 areas of the placenta for both the non-labor group and labor group (ANOVA p<0.05). For the non-labor group there was less HSP 27 in the inner compared with both the middle (p<0.05) and outer area (p<0.05). For the labor group there was also less HSP 27 in both the inner compared with the middle (p<0.02) and outer area (p<0.01). Thus HSP 27 is expressed in a spatial manner within the placenta and the distribution patterns are similar in labor and non-labor.


Heat shock protein 27 is spatially distributed in the human placenta and decreased during labor.

Abdulsid A, Fletcher A, Lyall F - PLoS ONE (2013)

Median optical densities for HSP 27 expression in three different placenta zones for all patients.The upper panel shows non-labor (n = 6 patients) and the lower panel shows labor (n = 6 patients). Samples were compared with the Mann Whitney U test. Values are shown as median and median absolute deviation. n..s., non-significant.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3750034&req=5

pone-0071127-g003: Median optical densities for HSP 27 expression in three different placenta zones for all patients.The upper panel shows non-labor (n = 6 patients) and the lower panel shows labor (n = 6 patients). Samples were compared with the Mann Whitney U test. Values are shown as median and median absolute deviation. n..s., non-significant.
Mentions: The first set of experiments was designed to test whether there was a difference in HSP 27 expression within an individual placenta in either labor or non-labor. Figure 2 shows HSP 27 expression in the four zones of the inner, middle and outer area sampled from the cord insertion point. The upper panel shows a placenta obtained from a non-laboring caesarean section delivery. The bottom panel shows a placenta obtained from a women who was in labor and delivered vaginally. Figure 3 shows the combined analysis of all the placentae for either non-labor (upper graph) or labor (lower graph). Overall there was a significant difference between the 3 areas of the placenta for both the non-labor group and labor group (ANOVA p<0.05). For the non-labor group there was less HSP 27 in the inner compared with both the middle (p<0.05) and outer area (p<0.05). For the labor group there was also less HSP 27 in both the inner compared with the middle (p<0.02) and outer area (p<0.01). Thus HSP 27 is expressed in a spatial manner within the placenta and the distribution patterns are similar in labor and non-labor.

Bottom Line: For labor, there was also less HSP 27 protein in the inner region compared with both the middle (p<0.02) and outer region (p<0.01).Similar to HSP 27 protein, there was less HSP 27 mRNA in the labor group in both the inner region (p<0.05) and middle region (p<0.02) compared to non-labor.The results have important implications for how data obtained from studies in the placenta can be influenced by sampling methods.

View Article: PubMed Central - PubMed

Affiliation: University of Glasgow School of Medicine, Institute of Medical Genetics, Yorkhill Hospital, Glasgow, United Kingdom.

ABSTRACT
Placental oxidative stress is a feature of human labor. Heat shock proteins (HSPs) play a key role in cellular stress. We hypothesized that placental expression of the small HSP 27 would be altered during labor and expression would vary in different regions of the placenta. Six women in labor who delivered vaginally and 6 women not in labor, who were delivered by Cesarean section, were recruited. Four equally spaced pieces were sampled from the inner, middle and outer regions of each placenta (total 12 samples per placenta). HSP 27 expression was investigated by Western blot analysis and RT-PCR. For non-labor, there was less HSP 27 protein in the inner placenta region compared with both the middle region (p<0.05) and outer region (p<0.05). For labor, there was also less HSP 27 protein in the inner region compared with both the middle (p<0.02) and outer region (p<0.01). When the 3 regions of the placenta were compared for non-labor versus labor there was less HSP 27 in the labor group at both the inner (p<0.05) and middle regions (p<0.005) compared to non-labor. Similar to HSP 27 protein, there was less HSP 27 mRNA in the labor group in both the inner region (p<0.05) and middle region (p<0.02) compared to non-labor. This study suggests that placental HSP 27 may play a role in labor and is spatially controlled. The results have important implications for how data obtained from studies in the placenta can be influenced by sampling methods.

Show MeSH
Related in: MedlinePlus