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Heat shock protein 27 is spatially distributed in the human placenta and decreased during labor.

Abdulsid A, Fletcher A, Lyall F - PLoS ONE (2013)

Bottom Line: For labor, there was also less HSP 27 protein in the inner region compared with both the middle (p<0.02) and outer region (p<0.01).Similar to HSP 27 protein, there was less HSP 27 mRNA in the labor group in both the inner region (p<0.05) and middle region (p<0.02) compared to non-labor.The results have important implications for how data obtained from studies in the placenta can be influenced by sampling methods.

View Article: PubMed Central - PubMed

Affiliation: University of Glasgow School of Medicine, Institute of Medical Genetics, Yorkhill Hospital, Glasgow, United Kingdom.

ABSTRACT
Placental oxidative stress is a feature of human labor. Heat shock proteins (HSPs) play a key role in cellular stress. We hypothesized that placental expression of the small HSP 27 would be altered during labor and expression would vary in different regions of the placenta. Six women in labor who delivered vaginally and 6 women not in labor, who were delivered by Cesarean section, were recruited. Four equally spaced pieces were sampled from the inner, middle and outer regions of each placenta (total 12 samples per placenta). HSP 27 expression was investigated by Western blot analysis and RT-PCR. For non-labor, there was less HSP 27 protein in the inner placenta region compared with both the middle region (p<0.05) and outer region (p<0.05). For labor, there was also less HSP 27 protein in the inner region compared with both the middle (p<0.02) and outer region (p<0.01). When the 3 regions of the placenta were compared for non-labor versus labor there was less HSP 27 in the labor group at both the inner (p<0.05) and middle regions (p<0.005) compared to non-labor. Similar to HSP 27 protein, there was less HSP 27 mRNA in the labor group in both the inner region (p<0.05) and middle region (p<0.02) compared to non-labor. This study suggests that placental HSP 27 may play a role in labor and is spatially controlled. The results have important implications for how data obtained from studies in the placenta can be influenced by sampling methods.

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Diagrammatic representation showing where samples were obtained from each individual placenta.
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pone-0071127-g001: Diagrammatic representation showing where samples were obtained from each individual placenta.

Mentions: For each patient (6 patients per group), placental samples (∼1 cm3) were obtained from three sites by taking measurements from the cord insertion point: inner third closest to cord insertion point (inner zone), middle of placenta (middle zone) and outer third of placenta (outer zone) of placenta. Within each zone four separate samples were obtained representing the four quadrants (Figure 1). Placentae had a central cord insertion. Samples were rinsed and immediately flash frozen in liquid nitrogen. For this study we had performed a power analysis using G*Power 3.1 for Macintosh and based the numbers on our previous published work [12].


Heat shock protein 27 is spatially distributed in the human placenta and decreased during labor.

Abdulsid A, Fletcher A, Lyall F - PLoS ONE (2013)

Diagrammatic representation showing where samples were obtained from each individual placenta.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3750034&req=5

pone-0071127-g001: Diagrammatic representation showing where samples were obtained from each individual placenta.
Mentions: For each patient (6 patients per group), placental samples (∼1 cm3) were obtained from three sites by taking measurements from the cord insertion point: inner third closest to cord insertion point (inner zone), middle of placenta (middle zone) and outer third of placenta (outer zone) of placenta. Within each zone four separate samples were obtained representing the four quadrants (Figure 1). Placentae had a central cord insertion. Samples were rinsed and immediately flash frozen in liquid nitrogen. For this study we had performed a power analysis using G*Power 3.1 for Macintosh and based the numbers on our previous published work [12].

Bottom Line: For labor, there was also less HSP 27 protein in the inner region compared with both the middle (p<0.02) and outer region (p<0.01).Similar to HSP 27 protein, there was less HSP 27 mRNA in the labor group in both the inner region (p<0.05) and middle region (p<0.02) compared to non-labor.The results have important implications for how data obtained from studies in the placenta can be influenced by sampling methods.

View Article: PubMed Central - PubMed

Affiliation: University of Glasgow School of Medicine, Institute of Medical Genetics, Yorkhill Hospital, Glasgow, United Kingdom.

ABSTRACT
Placental oxidative stress is a feature of human labor. Heat shock proteins (HSPs) play a key role in cellular stress. We hypothesized that placental expression of the small HSP 27 would be altered during labor and expression would vary in different regions of the placenta. Six women in labor who delivered vaginally and 6 women not in labor, who were delivered by Cesarean section, were recruited. Four equally spaced pieces were sampled from the inner, middle and outer regions of each placenta (total 12 samples per placenta). HSP 27 expression was investigated by Western blot analysis and RT-PCR. For non-labor, there was less HSP 27 protein in the inner placenta region compared with both the middle region (p<0.05) and outer region (p<0.05). For labor, there was also less HSP 27 protein in the inner region compared with both the middle (p<0.02) and outer region (p<0.01). When the 3 regions of the placenta were compared for non-labor versus labor there was less HSP 27 in the labor group at both the inner (p<0.05) and middle regions (p<0.005) compared to non-labor. Similar to HSP 27 protein, there was less HSP 27 mRNA in the labor group in both the inner region (p<0.05) and middle region (p<0.02) compared to non-labor. This study suggests that placental HSP 27 may play a role in labor and is spatially controlled. The results have important implications for how data obtained from studies in the placenta can be influenced by sampling methods.

Show MeSH
Related in: MedlinePlus