Limits...
A focus on the optical properties of the regenerated newt lens.

Wassmer S, Beddaoui M, Rajai P, Munger R, Tsilfidis C - PLoS ONE (2013)

Bottom Line: We compared the optical properties of 9- and 26-week regenerated lenses in the red spotted newt, Notophthalmus viridescens, with the original lenses removed from the same eyes.The 9 week lenses have greater light transmission, but significantly reduced focal length and refractive index than the original lenses.This suggests that following 9 weeks of regeneration, the lenses have not recovered the functionality of the original lens.

View Article: PubMed Central - PubMed

Affiliation: Ottawa Hospital Research Institute, Vision Research Program, Ottawa, Ontario, Canada ; Department of Cellular and Molecular Medicine, University of Ottawa, Ottawa, Ontario, Canada.

ABSTRACT
Lens regeneration studies in the adult newt suggest that molecular aspects of lens regeneration are complete within 5 weeks of lentectomy. However, very little is known about the optical properties of the regenerated lens. In an aquatic environment, the lens accounts for almost all of the refractive power of the eye, and thus, a fully functional lens is critical. We compared the optical properties of 9- and 26-week regenerated lenses in the red spotted newt, Notophthalmus viridescens, with the original lenses removed from the same eyes. At 9 weeks, the regenerated lenses are smaller than the original lenses and are histologically immature, with a lower density of lens proteins. The 9 week lenses have greater light transmission, but significantly reduced focal length and refractive index than the original lenses. This suggests that following 9 weeks of regeneration, the lenses have not recovered the functionality of the original lens. By 26 weeks, the transmission of light in the more mature lens is reduced, but the optical parameters of the lens have recovered enough to allow functional vision.

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Related in: MedlinePlus

Focal length measurements setup.A collimated red HeNe laser beam 10 mm in diameter is focused by a long working distance objective lens (Mitutoyo, 40x) positioned on a graduated translational stage (±0.05 mm) such that the focus point can be moved relative to the lens until it produces a focus point on a screen 116 mm from the bottom of the dish. The power and focal length of the lens can be calculated using the formulas shown.
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pone-0070845-g006: Focal length measurements setup.A collimated red HeNe laser beam 10 mm in diameter is focused by a long working distance objective lens (Mitutoyo, 40x) positioned on a graduated translational stage (±0.05 mm) such that the focus point can be moved relative to the lens until it produces a focus point on a screen 116 mm from the bottom of the dish. The power and focal length of the lens can be calculated using the formulas shown.

Mentions: Focal length measurements were performed within 30 minutes of the transmitted light measurements with the lens remaining in the original oil-filled Petri dish. The measurement was performed as per Figure 6 where a collimated red HeNe laser beam 10 mm in diameter is focused by a long working distance objective lens (Mitutoyo, 40x) positioned on a graduated translational stage (±0.05 mm) such that the focus point can be moved relative to the lens until it produces a focus point on a screen 116 mm from the bottom of the dish.


A focus on the optical properties of the regenerated newt lens.

Wassmer S, Beddaoui M, Rajai P, Munger R, Tsilfidis C - PLoS ONE (2013)

Focal length measurements setup.A collimated red HeNe laser beam 10 mm in diameter is focused by a long working distance objective lens (Mitutoyo, 40x) positioned on a graduated translational stage (±0.05 mm) such that the focus point can be moved relative to the lens until it produces a focus point on a screen 116 mm from the bottom of the dish. The power and focal length of the lens can be calculated using the formulas shown.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3750027&req=5

pone-0070845-g006: Focal length measurements setup.A collimated red HeNe laser beam 10 mm in diameter is focused by a long working distance objective lens (Mitutoyo, 40x) positioned on a graduated translational stage (±0.05 mm) such that the focus point can be moved relative to the lens until it produces a focus point on a screen 116 mm from the bottom of the dish. The power and focal length of the lens can be calculated using the formulas shown.
Mentions: Focal length measurements were performed within 30 minutes of the transmitted light measurements with the lens remaining in the original oil-filled Petri dish. The measurement was performed as per Figure 6 where a collimated red HeNe laser beam 10 mm in diameter is focused by a long working distance objective lens (Mitutoyo, 40x) positioned on a graduated translational stage (±0.05 mm) such that the focus point can be moved relative to the lens until it produces a focus point on a screen 116 mm from the bottom of the dish.

Bottom Line: We compared the optical properties of 9- and 26-week regenerated lenses in the red spotted newt, Notophthalmus viridescens, with the original lenses removed from the same eyes.The 9 week lenses have greater light transmission, but significantly reduced focal length and refractive index than the original lenses.This suggests that following 9 weeks of regeneration, the lenses have not recovered the functionality of the original lens.

View Article: PubMed Central - PubMed

Affiliation: Ottawa Hospital Research Institute, Vision Research Program, Ottawa, Ontario, Canada ; Department of Cellular and Molecular Medicine, University of Ottawa, Ottawa, Ontario, Canada.

ABSTRACT
Lens regeneration studies in the adult newt suggest that molecular aspects of lens regeneration are complete within 5 weeks of lentectomy. However, very little is known about the optical properties of the regenerated lens. In an aquatic environment, the lens accounts for almost all of the refractive power of the eye, and thus, a fully functional lens is critical. We compared the optical properties of 9- and 26-week regenerated lenses in the red spotted newt, Notophthalmus viridescens, with the original lenses removed from the same eyes. At 9 weeks, the regenerated lenses are smaller than the original lenses and are histologically immature, with a lower density of lens proteins. The 9 week lenses have greater light transmission, but significantly reduced focal length and refractive index than the original lenses. This suggests that following 9 weeks of regeneration, the lenses have not recovered the functionality of the original lens. By 26 weeks, the transmission of light in the more mature lens is reduced, but the optical parameters of the lens have recovered enough to allow functional vision.

Show MeSH
Related in: MedlinePlus