Limits...
A focus on the optical properties of the regenerated newt lens.

Wassmer S, Beddaoui M, Rajai P, Munger R, Tsilfidis C - PLoS ONE (2013)

Bottom Line: We compared the optical properties of 9- and 26-week regenerated lenses in the red spotted newt, Notophthalmus viridescens, with the original lenses removed from the same eyes.The 9 week lenses have greater light transmission, but significantly reduced focal length and refractive index than the original lenses.This suggests that following 9 weeks of regeneration, the lenses have not recovered the functionality of the original lens.

View Article: PubMed Central - PubMed

Affiliation: Ottawa Hospital Research Institute, Vision Research Program, Ottawa, Ontario, Canada ; Department of Cellular and Molecular Medicine, University of Ottawa, Ottawa, Ontario, Canada.

ABSTRACT
Lens regeneration studies in the adult newt suggest that molecular aspects of lens regeneration are complete within 5 weeks of lentectomy. However, very little is known about the optical properties of the regenerated lens. In an aquatic environment, the lens accounts for almost all of the refractive power of the eye, and thus, a fully functional lens is critical. We compared the optical properties of 9- and 26-week regenerated lenses in the red spotted newt, Notophthalmus viridescens, with the original lenses removed from the same eyes. At 9 weeks, the regenerated lenses are smaller than the original lenses and are histologically immature, with a lower density of lens proteins. The 9 week lenses have greater light transmission, but significantly reduced focal length and refractive index than the original lenses. This suggests that following 9 weeks of regeneration, the lenses have not recovered the functionality of the original lens. By 26 weeks, the transmission of light in the more mature lens is reduced, but the optical parameters of the lens have recovered enough to allow functional vision.

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The optical properties of the original and 9-week regenerated lenses.A) Lens diameter is significantly reduced in 9-week regenerates. B) Transmitted light of regenerated lens is higher than original lens. This is often typical of younger lenses. C) Focal length of regenerated lens is lower than original lens. D) Lens equivalent refractive index is significantly lower in regenerated the lens.
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pone-0070845-g001: The optical properties of the original and 9-week regenerated lenses.A) Lens diameter is significantly reduced in 9-week regenerates. B) Transmitted light of regenerated lens is higher than original lens. This is often typical of younger lenses. C) Focal length of regenerated lens is lower than original lens. D) Lens equivalent refractive index is significantly lower in regenerated the lens.

Mentions: In healthy adult newts, the normal lens is approximately 1 mm in diameter. The median for our group was 0.998 mm. After 9 weeks of regeneration, most regenerated lenses were <0.80 mm in diameter (median = 0.705, Figure 1A), a statistically significant difference (P<0.001). Individual values are presented in the supplemental material (Table S1).


A focus on the optical properties of the regenerated newt lens.

Wassmer S, Beddaoui M, Rajai P, Munger R, Tsilfidis C - PLoS ONE (2013)

The optical properties of the original and 9-week regenerated lenses.A) Lens diameter is significantly reduced in 9-week regenerates. B) Transmitted light of regenerated lens is higher than original lens. This is often typical of younger lenses. C) Focal length of regenerated lens is lower than original lens. D) Lens equivalent refractive index is significantly lower in regenerated the lens.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3750027&req=5

pone-0070845-g001: The optical properties of the original and 9-week regenerated lenses.A) Lens diameter is significantly reduced in 9-week regenerates. B) Transmitted light of regenerated lens is higher than original lens. This is often typical of younger lenses. C) Focal length of regenerated lens is lower than original lens. D) Lens equivalent refractive index is significantly lower in regenerated the lens.
Mentions: In healthy adult newts, the normal lens is approximately 1 mm in diameter. The median for our group was 0.998 mm. After 9 weeks of regeneration, most regenerated lenses were <0.80 mm in diameter (median = 0.705, Figure 1A), a statistically significant difference (P<0.001). Individual values are presented in the supplemental material (Table S1).

Bottom Line: We compared the optical properties of 9- and 26-week regenerated lenses in the red spotted newt, Notophthalmus viridescens, with the original lenses removed from the same eyes.The 9 week lenses have greater light transmission, but significantly reduced focal length and refractive index than the original lenses.This suggests that following 9 weeks of regeneration, the lenses have not recovered the functionality of the original lens.

View Article: PubMed Central - PubMed

Affiliation: Ottawa Hospital Research Institute, Vision Research Program, Ottawa, Ontario, Canada ; Department of Cellular and Molecular Medicine, University of Ottawa, Ottawa, Ontario, Canada.

ABSTRACT
Lens regeneration studies in the adult newt suggest that molecular aspects of lens regeneration are complete within 5 weeks of lentectomy. However, very little is known about the optical properties of the regenerated lens. In an aquatic environment, the lens accounts for almost all of the refractive power of the eye, and thus, a fully functional lens is critical. We compared the optical properties of 9- and 26-week regenerated lenses in the red spotted newt, Notophthalmus viridescens, with the original lenses removed from the same eyes. At 9 weeks, the regenerated lenses are smaller than the original lenses and are histologically immature, with a lower density of lens proteins. The 9 week lenses have greater light transmission, but significantly reduced focal length and refractive index than the original lenses. This suggests that following 9 weeks of regeneration, the lenses have not recovered the functionality of the original lens. By 26 weeks, the transmission of light in the more mature lens is reduced, but the optical parameters of the lens have recovered enough to allow functional vision.

Show MeSH
Related in: MedlinePlus