Limits...
Structural, antigenic, and evolutionary characterizations of the envelope protein of newly emerging Duck Tembusu Virus.

Yu K, Sheng ZZ, Huang B, Ma X, Li Y, Yuan X, Qin Z, Wang D, Chakravarty S, Li F, Song M, Sun H - PLoS ONE (2013)

Bottom Line: Among the six DTMUV strains, mutations were observed only at thirteen amino acid positions across three separate domains of the E protein.Interestingly, these genetic polymorphisms resulted in no detectable change in viral neutralization properties as demonstrated in a serum neutralization assay.New findings described here shall give insights into the antigenicity and evolution of this new pathogen and provide guidance for further functional studies of the E protein for which no effective vaccine has yet been developed.

View Article: PubMed Central - PubMed

Affiliation: College of Veterinary Medicine, Yangzhou University, Yangzhou, China ; Institute of Poultry Science, Shandong Academy of Agricultural Sciences, Jinan, China.

ABSTRACT
Since the first reported cases of ducks infected with a previously unknown flavivirus in eastern China in April 2010, the virus, provisionally designated Duck Tembusu Virus (DTMUV), has spread widely in domestic ducks in China and caused significant economic losses to poultry industry. In this study, we examined in detail structural, antigenic, and evolutionary properties of envelope (E) proteins of six DTMUV isolates spanning 2010-2012, each being isolated from individual farms with different geographical locations where disease outbreaks were documented. Structural analysis showed that E proteins of DTMUV and its closely related flavivirus (Japanese Encephalitis Virus) shared a conserved array of predicted functional domains and motifs. Among the six DTMUV strains, mutations were observed only at thirteen amino acid positions across three separate domains of the E protein. Interestingly, these genetic polymorphisms resulted in no detectable change in viral neutralization properties as demonstrated in a serum neutralization assay. Furthermore, phylogenetic analysis of the nucleotide sequences of the E proteins showed that viruses evolved into two distinct genotypes, termed as DTMUV.I and DTMUV.II, with II emerging as the dominant genotype. New findings described here shall give insights into the antigenicity and evolution of this new pathogen and provide guidance for further functional studies of the E protein for which no effective vaccine has yet been developed.

Show MeSH

Related in: MedlinePlus

Geographical location of poultry farms from which six virus isolates were obtained.
© Copyright Policy
Related In: Results  -  Collection


getmorefigures.php?uid=PMC3750017&req=5

pone-0071319-g001: Geographical location of poultry farms from which six virus isolates were obtained.

Mentions: Clinical samples were collected from six poultry farms that experienced disease outbreaks from 2010 to 2012. Three farms are located in three different provinces, while the other three farms are within one province but have different geographical locations. This information was summarized in Table 1 and Figure 1. Brain or ovary tissues from the diseased ducks were homogenized in sterile phosphate-buffered saline(PBS, pH 7.2)to form a 20% suspension(w/v. After centrifugation at 8000×g for 30 min, the supernatants were filtered by 0.2 µm-pore-size syringe-driven filters. The filtered suspensions were then inoculated into 10-day-old healthy DTMUV antibody-free duck embryonic eggs via allantochorion. Embryonic eggs were examined daily, and the allantoic fluids of dead embryonic eggs were collected after one more passage. The viral RNA was extracted from the allantoic fluids with the MiniBEST viral RNA Extraction kit(TaKaRa).


Structural, antigenic, and evolutionary characterizations of the envelope protein of newly emerging Duck Tembusu Virus.

Yu K, Sheng ZZ, Huang B, Ma X, Li Y, Yuan X, Qin Z, Wang D, Chakravarty S, Li F, Song M, Sun H - PLoS ONE (2013)

Geographical location of poultry farms from which six virus isolates were obtained.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3750017&req=5

pone-0071319-g001: Geographical location of poultry farms from which six virus isolates were obtained.
Mentions: Clinical samples were collected from six poultry farms that experienced disease outbreaks from 2010 to 2012. Three farms are located in three different provinces, while the other three farms are within one province but have different geographical locations. This information was summarized in Table 1 and Figure 1. Brain or ovary tissues from the diseased ducks were homogenized in sterile phosphate-buffered saline(PBS, pH 7.2)to form a 20% suspension(w/v. After centrifugation at 8000×g for 30 min, the supernatants were filtered by 0.2 µm-pore-size syringe-driven filters. The filtered suspensions were then inoculated into 10-day-old healthy DTMUV antibody-free duck embryonic eggs via allantochorion. Embryonic eggs were examined daily, and the allantoic fluids of dead embryonic eggs were collected after one more passage. The viral RNA was extracted from the allantoic fluids with the MiniBEST viral RNA Extraction kit(TaKaRa).

Bottom Line: Among the six DTMUV strains, mutations were observed only at thirteen amino acid positions across three separate domains of the E protein.Interestingly, these genetic polymorphisms resulted in no detectable change in viral neutralization properties as demonstrated in a serum neutralization assay.New findings described here shall give insights into the antigenicity and evolution of this new pathogen and provide guidance for further functional studies of the E protein for which no effective vaccine has yet been developed.

View Article: PubMed Central - PubMed

Affiliation: College of Veterinary Medicine, Yangzhou University, Yangzhou, China ; Institute of Poultry Science, Shandong Academy of Agricultural Sciences, Jinan, China.

ABSTRACT
Since the first reported cases of ducks infected with a previously unknown flavivirus in eastern China in April 2010, the virus, provisionally designated Duck Tembusu Virus (DTMUV), has spread widely in domestic ducks in China and caused significant economic losses to poultry industry. In this study, we examined in detail structural, antigenic, and evolutionary properties of envelope (E) proteins of six DTMUV isolates spanning 2010-2012, each being isolated from individual farms with different geographical locations where disease outbreaks were documented. Structural analysis showed that E proteins of DTMUV and its closely related flavivirus (Japanese Encephalitis Virus) shared a conserved array of predicted functional domains and motifs. Among the six DTMUV strains, mutations were observed only at thirteen amino acid positions across three separate domains of the E protein. Interestingly, these genetic polymorphisms resulted in no detectable change in viral neutralization properties as demonstrated in a serum neutralization assay. Furthermore, phylogenetic analysis of the nucleotide sequences of the E proteins showed that viruses evolved into two distinct genotypes, termed as DTMUV.I and DTMUV.II, with II emerging as the dominant genotype. New findings described here shall give insights into the antigenicity and evolution of this new pathogen and provide guidance for further functional studies of the E protein for which no effective vaccine has yet been developed.

Show MeSH
Related in: MedlinePlus