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Hydrocellular foam dressing promotes wound healing along with increases in hyaluronan synthase 3 and PPARα gene expression in epidermis.

Yamane T, Nakagami G, Yoshino S, Muramatsu A, Matsui S, Oishi Y, Kanazawa T, Minematsu T, Sanada H - PLoS ONE (2013)

Bottom Line: We created two full-thickness wounds on the dorsolateral skin of rats.Each wound was covered with either a hydrocellular foam dressing or a film dressing and hyaluronan levels in the periwound skin was measured.In addition, hydrocellular foam dressing enhanced the mRNA levels of PPARα, which upregulates Has3 gene expression, and the major hyaluronan receptor CD44.

View Article: PubMed Central - PubMed

Affiliation: Department of Gerontological Nursing/Wound Care Management, Graduate School of Medicine, the University of Tokyo, Tokyo, Japan.

ABSTRACT

Background: Hydrocellular foam dressing, modern wound dressing, induces moist wound environment and promotes wound healing: however, the regulatory mechanisms responsible for these effects are poorly understood. This study was aimed to reveal the effect of hydrocellular foam dressing on hyaluronan, which has been shown to have positive effects on wound healing, and examined its regulatory mechanisms in rat skin.

Methodology/principal findings: We created two full-thickness wounds on the dorsolateral skin of rats. Each wound was covered with either a hydrocellular foam dressing or a film dressing and hyaluronan levels in the periwound skin was measured. We also investigated the mechanism by which the hydrocellular foam dressing regulates hyaluronan production by measuring the gene expression of hyaluronan synthase 3 (Has3), peroxisome proliferator-activated receptor α (PPARα), and CD44. Hydrocellular foam dressing promoted wound healing and upregulated hyaluronan synthesis, along with an increase in the mRNA levels of Has3, which plays a primary role in hyaluronan synthesis in epidermis. In addition, hydrocellular foam dressing enhanced the mRNA levels of PPARα, which upregulates Has3 gene expression, and the major hyaluronan receptor CD44.

Conclusions/significance: These findings suggests that hydrocellular foam dressing may be beneficial for wound healing along with increases in hyaluronan synthase 3 and PPARα gene expression in epidermis. We believe that the present study would contribute to the elucidation of the mechanisms underlying the effects of hydrocellular foam dressing-induced moist environment on wound healing and practice evidence-based wound care.

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Effects of hydrocellular foam dressing on PPARα mRNA levels in periwound epidermis.The PPARα mRNA levels in periwound epidermis were measured by quantitative PCR and expressed as values relative to those of GAPDH. Bars are expressed as mean ± SE (n = 5). *p < 0.05 indicates values that are significantly different from the side of film dressing in rat skin.
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pone-0073988-g004: Effects of hydrocellular foam dressing on PPARα mRNA levels in periwound epidermis.The PPARα mRNA levels in periwound epidermis were measured by quantitative PCR and expressed as values relative to those of GAPDH. Bars are expressed as mean ± SE (n = 5). *p < 0.05 indicates values that are significantly different from the side of film dressing in rat skin.

Mentions: Our preliminary experiments showed that PPARα agonist WY14,643 enhanced Has3 mRNA expression in human keratinocytes (data not shown). We therefore investigated PPARα mRNA levels in epidermis around the wound (Figure 4). PPARα mRNA levels were significantly higher around wounds covered with hydrocellular foam dressings, compared with those covered with film dressings.


Hydrocellular foam dressing promotes wound healing along with increases in hyaluronan synthase 3 and PPARα gene expression in epidermis.

Yamane T, Nakagami G, Yoshino S, Muramatsu A, Matsui S, Oishi Y, Kanazawa T, Minematsu T, Sanada H - PLoS ONE (2013)

Effects of hydrocellular foam dressing on PPARα mRNA levels in periwound epidermis.The PPARα mRNA levels in periwound epidermis were measured by quantitative PCR and expressed as values relative to those of GAPDH. Bars are expressed as mean ± SE (n = 5). *p < 0.05 indicates values that are significantly different from the side of film dressing in rat skin.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3750004&req=5

pone-0073988-g004: Effects of hydrocellular foam dressing on PPARα mRNA levels in periwound epidermis.The PPARα mRNA levels in periwound epidermis were measured by quantitative PCR and expressed as values relative to those of GAPDH. Bars are expressed as mean ± SE (n = 5). *p < 0.05 indicates values that are significantly different from the side of film dressing in rat skin.
Mentions: Our preliminary experiments showed that PPARα agonist WY14,643 enhanced Has3 mRNA expression in human keratinocytes (data not shown). We therefore investigated PPARα mRNA levels in epidermis around the wound (Figure 4). PPARα mRNA levels were significantly higher around wounds covered with hydrocellular foam dressings, compared with those covered with film dressings.

Bottom Line: We created two full-thickness wounds on the dorsolateral skin of rats.Each wound was covered with either a hydrocellular foam dressing or a film dressing and hyaluronan levels in the periwound skin was measured.In addition, hydrocellular foam dressing enhanced the mRNA levels of PPARα, which upregulates Has3 gene expression, and the major hyaluronan receptor CD44.

View Article: PubMed Central - PubMed

Affiliation: Department of Gerontological Nursing/Wound Care Management, Graduate School of Medicine, the University of Tokyo, Tokyo, Japan.

ABSTRACT

Background: Hydrocellular foam dressing, modern wound dressing, induces moist wound environment and promotes wound healing: however, the regulatory mechanisms responsible for these effects are poorly understood. This study was aimed to reveal the effect of hydrocellular foam dressing on hyaluronan, which has been shown to have positive effects on wound healing, and examined its regulatory mechanisms in rat skin.

Methodology/principal findings: We created two full-thickness wounds on the dorsolateral skin of rats. Each wound was covered with either a hydrocellular foam dressing or a film dressing and hyaluronan levels in the periwound skin was measured. We also investigated the mechanism by which the hydrocellular foam dressing regulates hyaluronan production by measuring the gene expression of hyaluronan synthase 3 (Has3), peroxisome proliferator-activated receptor α (PPARα), and CD44. Hydrocellular foam dressing promoted wound healing and upregulated hyaluronan synthesis, along with an increase in the mRNA levels of Has3, which plays a primary role in hyaluronan synthesis in epidermis. In addition, hydrocellular foam dressing enhanced the mRNA levels of PPARα, which upregulates Has3 gene expression, and the major hyaluronan receptor CD44.

Conclusions/significance: These findings suggests that hydrocellular foam dressing may be beneficial for wound healing along with increases in hyaluronan synthase 3 and PPARα gene expression in epidermis. We believe that the present study would contribute to the elucidation of the mechanisms underlying the effects of hydrocellular foam dressing-induced moist environment on wound healing and practice evidence-based wound care.

Show MeSH
Related in: MedlinePlus