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Hydrocellular foam dressing promotes wound healing along with increases in hyaluronan synthase 3 and PPARα gene expression in epidermis.

Yamane T, Nakagami G, Yoshino S, Muramatsu A, Matsui S, Oishi Y, Kanazawa T, Minematsu T, Sanada H - PLoS ONE (2013)

Bottom Line: We created two full-thickness wounds on the dorsolateral skin of rats.Each wound was covered with either a hydrocellular foam dressing or a film dressing and hyaluronan levels in the periwound skin was measured.In addition, hydrocellular foam dressing enhanced the mRNA levels of PPARα, which upregulates Has3 gene expression, and the major hyaluronan receptor CD44.

View Article: PubMed Central - PubMed

Affiliation: Department of Gerontological Nursing/Wound Care Management, Graduate School of Medicine, the University of Tokyo, Tokyo, Japan.

ABSTRACT

Background: Hydrocellular foam dressing, modern wound dressing, induces moist wound environment and promotes wound healing: however, the regulatory mechanisms responsible for these effects are poorly understood. This study was aimed to reveal the effect of hydrocellular foam dressing on hyaluronan, which has been shown to have positive effects on wound healing, and examined its regulatory mechanisms in rat skin.

Methodology/principal findings: We created two full-thickness wounds on the dorsolateral skin of rats. Each wound was covered with either a hydrocellular foam dressing or a film dressing and hyaluronan levels in the periwound skin was measured. We also investigated the mechanism by which the hydrocellular foam dressing regulates hyaluronan production by measuring the gene expression of hyaluronan synthase 3 (Has3), peroxisome proliferator-activated receptor α (PPARα), and CD44. Hydrocellular foam dressing promoted wound healing and upregulated hyaluronan synthesis, along with an increase in the mRNA levels of Has3, which plays a primary role in hyaluronan synthesis in epidermis. In addition, hydrocellular foam dressing enhanced the mRNA levels of PPARα, which upregulates Has3 gene expression, and the major hyaluronan receptor CD44.

Conclusions/significance: These findings suggests that hydrocellular foam dressing may be beneficial for wound healing along with increases in hyaluronan synthase 3 and PPARα gene expression in epidermis. We believe that the present study would contribute to the elucidation of the mechanisms underlying the effects of hydrocellular foam dressing-induced moist environment on wound healing and practice evidence-based wound care.

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Related in: MedlinePlus

Full-thickness cutaneous wound model.Two full-thickness wounds, a diameter of 1.5 cm, were created in the dorsolateral skin of five rats using sterile scissors. Each wound was covered with either a hydrocellular foam dressing (A) or the film dressing (B). A film dressing was used as a secondary dressing in both sides of individual rat and also used to keep the hydrocellular foam dressing attached to the wound.
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pone-0073988-g001: Full-thickness cutaneous wound model.Two full-thickness wounds, a diameter of 1.5 cm, were created in the dorsolateral skin of five rats using sterile scissors. Each wound was covered with either a hydrocellular foam dressing (A) or the film dressing (B). A film dressing was used as a secondary dressing in both sides of individual rat and also used to keep the hydrocellular foam dressing attached to the wound.

Mentions: Male specific-pathogen-free 7-week-old Wistar rats were purchased from Japan SLC Inc. (Shizuoka, Japan). Animals were given food and ultrafiltered water ad libitum, and were maintained on a 12-h/12-h light/dark cycle. Two full-thickness wounds, a diameter of 1.5 cm, were created in the dorsolateral skin of five rats using sterile scissors, under sedation with an intraperitoneal injection of Somnopentyl (pentobarbital sodium; Kyoritsu Seiyaku Corporation, Tokyo, Japan) (30 mg/kg body weight). The subcutaneous fat layer was completely dissected to expose the fascia. Each wound was covered with either a hydrocellular foam dressing (Figure 1A) or the film dressing (Figure 1B). A film dressing was used as a secondary dressing in both sides of individual rat and also used to keep the hydrocellular foam dressing attached to the wound. The wound area was measured every day until 7 days after wounding using image analysis software (IMAGEJ version 1.42; NIH, Bethesda, MD), and the results were expressed relative to the initial wound area. Periwound skin samples (5 mm thick, taken from the edge of the wound) were collected on day 4 under anesthesia with an intraperitoneal injection of Somnopentyl (30 mg/kg body weight) and were subjected to quantification of hyaluronan or RNA preparation. The experimental protocol was approved by the Animal Research Committee of The University of Tokyo. All animals were treated according to the Guide for the Care and Use of Laboratory Animals of the NIH.


Hydrocellular foam dressing promotes wound healing along with increases in hyaluronan synthase 3 and PPARα gene expression in epidermis.

Yamane T, Nakagami G, Yoshino S, Muramatsu A, Matsui S, Oishi Y, Kanazawa T, Minematsu T, Sanada H - PLoS ONE (2013)

Full-thickness cutaneous wound model.Two full-thickness wounds, a diameter of 1.5 cm, were created in the dorsolateral skin of five rats using sterile scissors. Each wound was covered with either a hydrocellular foam dressing (A) or the film dressing (B). A film dressing was used as a secondary dressing in both sides of individual rat and also used to keep the hydrocellular foam dressing attached to the wound.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3750004&req=5

pone-0073988-g001: Full-thickness cutaneous wound model.Two full-thickness wounds, a diameter of 1.5 cm, were created in the dorsolateral skin of five rats using sterile scissors. Each wound was covered with either a hydrocellular foam dressing (A) or the film dressing (B). A film dressing was used as a secondary dressing in both sides of individual rat and also used to keep the hydrocellular foam dressing attached to the wound.
Mentions: Male specific-pathogen-free 7-week-old Wistar rats were purchased from Japan SLC Inc. (Shizuoka, Japan). Animals were given food and ultrafiltered water ad libitum, and were maintained on a 12-h/12-h light/dark cycle. Two full-thickness wounds, a diameter of 1.5 cm, were created in the dorsolateral skin of five rats using sterile scissors, under sedation with an intraperitoneal injection of Somnopentyl (pentobarbital sodium; Kyoritsu Seiyaku Corporation, Tokyo, Japan) (30 mg/kg body weight). The subcutaneous fat layer was completely dissected to expose the fascia. Each wound was covered with either a hydrocellular foam dressing (Figure 1A) or the film dressing (Figure 1B). A film dressing was used as a secondary dressing in both sides of individual rat and also used to keep the hydrocellular foam dressing attached to the wound. The wound area was measured every day until 7 days after wounding using image analysis software (IMAGEJ version 1.42; NIH, Bethesda, MD), and the results were expressed relative to the initial wound area. Periwound skin samples (5 mm thick, taken from the edge of the wound) were collected on day 4 under anesthesia with an intraperitoneal injection of Somnopentyl (30 mg/kg body weight) and were subjected to quantification of hyaluronan or RNA preparation. The experimental protocol was approved by the Animal Research Committee of The University of Tokyo. All animals were treated according to the Guide for the Care and Use of Laboratory Animals of the NIH.

Bottom Line: We created two full-thickness wounds on the dorsolateral skin of rats.Each wound was covered with either a hydrocellular foam dressing or a film dressing and hyaluronan levels in the periwound skin was measured.In addition, hydrocellular foam dressing enhanced the mRNA levels of PPARα, which upregulates Has3 gene expression, and the major hyaluronan receptor CD44.

View Article: PubMed Central - PubMed

Affiliation: Department of Gerontological Nursing/Wound Care Management, Graduate School of Medicine, the University of Tokyo, Tokyo, Japan.

ABSTRACT

Background: Hydrocellular foam dressing, modern wound dressing, induces moist wound environment and promotes wound healing: however, the regulatory mechanisms responsible for these effects are poorly understood. This study was aimed to reveal the effect of hydrocellular foam dressing on hyaluronan, which has been shown to have positive effects on wound healing, and examined its regulatory mechanisms in rat skin.

Methodology/principal findings: We created two full-thickness wounds on the dorsolateral skin of rats. Each wound was covered with either a hydrocellular foam dressing or a film dressing and hyaluronan levels in the periwound skin was measured. We also investigated the mechanism by which the hydrocellular foam dressing regulates hyaluronan production by measuring the gene expression of hyaluronan synthase 3 (Has3), peroxisome proliferator-activated receptor α (PPARα), and CD44. Hydrocellular foam dressing promoted wound healing and upregulated hyaluronan synthesis, along with an increase in the mRNA levels of Has3, which plays a primary role in hyaluronan synthesis in epidermis. In addition, hydrocellular foam dressing enhanced the mRNA levels of PPARα, which upregulates Has3 gene expression, and the major hyaluronan receptor CD44.

Conclusions/significance: These findings suggests that hydrocellular foam dressing may be beneficial for wound healing along with increases in hyaluronan synthase 3 and PPARα gene expression in epidermis. We believe that the present study would contribute to the elucidation of the mechanisms underlying the effects of hydrocellular foam dressing-induced moist environment on wound healing and practice evidence-based wound care.

Show MeSH
Related in: MedlinePlus