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In vitro proliferation and osteogenic differentiation of mesenchymal stem cells on nanoporous alumina.

Song Y, Ju Y, Song G, Morita Y - Int J Nanomedicine (2013)

Bottom Line: An MTT assay was used to measure cell viability of MSCs on different substrates, and determined that cell viability decreased with increasing pore size.Higher levels of osteoblastic differentiation markers such as alkaline phosphatase, osteocalcin, and mineralization were detected in cells cultured on alumina with 100 nm pores compared with cells cultured on alumina with either 20 nm pores or smooth alumina.This work demonstrates that cellular behavior is affected by variation in pore size, providing new insight into the potential application of this novel biocompatible material for the developing field of tissue engineering.

View Article: PubMed Central - PubMed

Affiliation: Department of Mechanical Science and Engineering, Nagoya University, Chikusa-ku, Nagoya, Japan.

ABSTRACT
Cell adhesion, migration, and proliferation are significantly affected by the surface topography of the substrates on which the cells are cultured. Alumina is one of the most popular implant materials used in orthopedics, but few data are available concerning the cellular responses of mesenchymal stem cells (MSCs) grown on nanoporous structures. MSCs were cultured on smooth alumina substrates and nanoporous alumina substrates to investigate the interaction between surface topographies of nanoporous alumina and cellular behavior. Nanoporous alumina substrates with pore sizes of 20 nm and 100 nm were used to evaluate the effect of pore size on MSCs as measured by proliferation, morphology, expression of integrin β1, and osteogenic differentiation. An MTT assay was used to measure cell viability of MSCs on different substrates, and determined that cell viability decreased with increasing pore size. Scanning electron microscopy was used to investigate the effect of pore size on cell morphology. Extremely elongated cells and prominent cell membrane protrusions were observed in cells cultured on alumina with the larger pore size. The expression of integrin β1 was enhanced in MSCs cultured on porous alumina, revealing that porous alumina substrates were more favorable for cell growth than smooth alumina substrates. Higher levels of osteoblastic differentiation markers such as alkaline phosphatase, osteocalcin, and mineralization were detected in cells cultured on alumina with 100 nm pores compared with cells cultured on alumina with either 20 nm pores or smooth alumina. This work demonstrates that cellular behavior is affected by variation in pore size, providing new insight into the potential application of this novel biocompatible material for the developing field of tissue engineering.

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Effect of nanoporous alumina substrate on cell viability. Mesenchymal stem cells were cultured on nanoporous alumina or smooth alumina surfaces for 1, 4, and 7 days and cell viability was measured using an MTT assay. *P < 0.05 denotes a significant difference between the smooth alumina and nanoporous alumina.
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f3-ijn-8-2745: Effect of nanoporous alumina substrate on cell viability. Mesenchymal stem cells were cultured on nanoporous alumina or smooth alumina surfaces for 1, 4, and 7 days and cell viability was measured using an MTT assay. *P < 0.05 denotes a significant difference between the smooth alumina and nanoporous alumina.

Mentions: An MTT assay was used to evaluate the viability of MSCs cultured on different substrates. The representative absorbance of the MSCs is shown in Figure 3. MSCs cultured on nanoporous alumina substrates with either 20 nm pores or 100 nm pores showed significantly higher cell viability (P < 0.05) than those cultured on smooth alumina after incubation for 4 days and 7 days. This result indicates that porous alumina is advantageous for cell growth. However, cell viability decreased with increasing pore size.


In vitro proliferation and osteogenic differentiation of mesenchymal stem cells on nanoporous alumina.

Song Y, Ju Y, Song G, Morita Y - Int J Nanomedicine (2013)

Effect of nanoporous alumina substrate on cell viability. Mesenchymal stem cells were cultured on nanoporous alumina or smooth alumina surfaces for 1, 4, and 7 days and cell viability was measured using an MTT assay. *P < 0.05 denotes a significant difference between the smooth alumina and nanoporous alumina.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3735283&req=5

f3-ijn-8-2745: Effect of nanoporous alumina substrate on cell viability. Mesenchymal stem cells were cultured on nanoporous alumina or smooth alumina surfaces for 1, 4, and 7 days and cell viability was measured using an MTT assay. *P < 0.05 denotes a significant difference between the smooth alumina and nanoporous alumina.
Mentions: An MTT assay was used to evaluate the viability of MSCs cultured on different substrates. The representative absorbance of the MSCs is shown in Figure 3. MSCs cultured on nanoporous alumina substrates with either 20 nm pores or 100 nm pores showed significantly higher cell viability (P < 0.05) than those cultured on smooth alumina after incubation for 4 days and 7 days. This result indicates that porous alumina is advantageous for cell growth. However, cell viability decreased with increasing pore size.

Bottom Line: An MTT assay was used to measure cell viability of MSCs on different substrates, and determined that cell viability decreased with increasing pore size.Higher levels of osteoblastic differentiation markers such as alkaline phosphatase, osteocalcin, and mineralization were detected in cells cultured on alumina with 100 nm pores compared with cells cultured on alumina with either 20 nm pores or smooth alumina.This work demonstrates that cellular behavior is affected by variation in pore size, providing new insight into the potential application of this novel biocompatible material for the developing field of tissue engineering.

View Article: PubMed Central - PubMed

Affiliation: Department of Mechanical Science and Engineering, Nagoya University, Chikusa-ku, Nagoya, Japan.

ABSTRACT
Cell adhesion, migration, and proliferation are significantly affected by the surface topography of the substrates on which the cells are cultured. Alumina is one of the most popular implant materials used in orthopedics, but few data are available concerning the cellular responses of mesenchymal stem cells (MSCs) grown on nanoporous structures. MSCs were cultured on smooth alumina substrates and nanoporous alumina substrates to investigate the interaction between surface topographies of nanoporous alumina and cellular behavior. Nanoporous alumina substrates with pore sizes of 20 nm and 100 nm were used to evaluate the effect of pore size on MSCs as measured by proliferation, morphology, expression of integrin β1, and osteogenic differentiation. An MTT assay was used to measure cell viability of MSCs on different substrates, and determined that cell viability decreased with increasing pore size. Scanning electron microscopy was used to investigate the effect of pore size on cell morphology. Extremely elongated cells and prominent cell membrane protrusions were observed in cells cultured on alumina with the larger pore size. The expression of integrin β1 was enhanced in MSCs cultured on porous alumina, revealing that porous alumina substrates were more favorable for cell growth than smooth alumina substrates. Higher levels of osteoblastic differentiation markers such as alkaline phosphatase, osteocalcin, and mineralization were detected in cells cultured on alumina with 100 nm pores compared with cells cultured on alumina with either 20 nm pores or smooth alumina. This work demonstrates that cellular behavior is affected by variation in pore size, providing new insight into the potential application of this novel biocompatible material for the developing field of tissue engineering.

Show MeSH
Related in: MedlinePlus