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Preliminary molecular identification of a predatory bug, Orius albidipennis, collected from ornamental plants.

Sayed SM, Montaser MM, Elsayed G, Amer SA - J. Insect Sci. (2013)

Bottom Line: The complete sequence of ITS1 and fragments of its flanking 18S and 5.8S rDNA genes are reported herein.The estimated length of ITS1 of O. albidipennis was 305 bp.The phylogentic relationships were determined using the maximum-likelihood method supported with strong bootstrap probabilities clustering of both taxa together.

View Article: PubMed Central - PubMed

Affiliation: Faculty of Science, Taif University, Kingdom of Saudi Arabia.

ABSTRACT
Orius albidipennis (Reuter) (Hemiptera: Anthocoridae) is a generalist predator used for biological control of insects attacking ornamental plants. Molecular identification of this species using internal transcribed spacer 1 (ITS1) of ribosomal DNA was conducted for the first time. The complete sequence of ITS1 and fragments of its flanking 18S and 5.8S rDNA genes are reported herein. The estimated length of ITS1 of O. albidipennis was 305 bp. This spacer was nearly identical to its counterpart of Orius sp-Taif strain in spite of the difference in their length. The phylogentic relationships were determined using the maximum-likelihood method supported with strong bootstrap probabilities clustering of both taxa together. Further molecular markers could be useful to identify the Taif strain and support its sister relationship to the Egyptian O. albidipennis.

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Features of the sequenced fragment of 18S rDNA gene, ITSI, and 5.8S rDNA gene from Orius albidipennis. High quality figures are available online.
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f01_01: Features of the sequenced fragment of 18S rDNA gene, ITSI, and 5.8S rDNA gene from Orius albidipennis. High quality figures are available online.

Mentions: Using the primers of Hinomoto et al. (2004), O. albidipennis gave a PCR product that showed similar size to its counterpart of some Orius species (data not shown). Sequencing of the PCR product showed that the amplified fragment acquired the typical arrangement of all Orius species sequenced so far. The first 131 bp were located in 3′ end of 18S rRNA gene, followed by 306 bp for the complete ITS1 and 74 bp for the 5′ end of 5.8S rRNA gene (Figure 1). The nucleotide sequence data reported in this paper will appear in the DDBJ/EMBL/GenBank nucleotide sequence databases with the accession number AB624550.


Preliminary molecular identification of a predatory bug, Orius albidipennis, collected from ornamental plants.

Sayed SM, Montaser MM, Elsayed G, Amer SA - J. Insect Sci. (2013)

Features of the sequenced fragment of 18S rDNA gene, ITSI, and 5.8S rDNA gene from Orius albidipennis. High quality figures are available online.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3735117&req=5

f01_01: Features of the sequenced fragment of 18S rDNA gene, ITSI, and 5.8S rDNA gene from Orius albidipennis. High quality figures are available online.
Mentions: Using the primers of Hinomoto et al. (2004), O. albidipennis gave a PCR product that showed similar size to its counterpart of some Orius species (data not shown). Sequencing of the PCR product showed that the amplified fragment acquired the typical arrangement of all Orius species sequenced so far. The first 131 bp were located in 3′ end of 18S rRNA gene, followed by 306 bp for the complete ITS1 and 74 bp for the 5′ end of 5.8S rRNA gene (Figure 1). The nucleotide sequence data reported in this paper will appear in the DDBJ/EMBL/GenBank nucleotide sequence databases with the accession number AB624550.

Bottom Line: The complete sequence of ITS1 and fragments of its flanking 18S and 5.8S rDNA genes are reported herein.The estimated length of ITS1 of O. albidipennis was 305 bp.The phylogentic relationships were determined using the maximum-likelihood method supported with strong bootstrap probabilities clustering of both taxa together.

View Article: PubMed Central - PubMed

Affiliation: Faculty of Science, Taif University, Kingdom of Saudi Arabia.

ABSTRACT
Orius albidipennis (Reuter) (Hemiptera: Anthocoridae) is a generalist predator used for biological control of insects attacking ornamental plants. Molecular identification of this species using internal transcribed spacer 1 (ITS1) of ribosomal DNA was conducted for the first time. The complete sequence of ITS1 and fragments of its flanking 18S and 5.8S rDNA genes are reported herein. The estimated length of ITS1 of O. albidipennis was 305 bp. This spacer was nearly identical to its counterpart of Orius sp-Taif strain in spite of the difference in their length. The phylogentic relationships were determined using the maximum-likelihood method supported with strong bootstrap probabilities clustering of both taxa together. Further molecular markers could be useful to identify the Taif strain and support its sister relationship to the Egyptian O. albidipennis.

Show MeSH
Related in: MedlinePlus