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Identification of tetrodotoxin-producing Shewanella spp. from feces of food poisoning patients and food samples.

Wang D, Wang Y, Huang H, Lin J, Xiao D, Kan B - Gut Pathog (2013)

Bottom Line: Shewanella spp. is infrequently recovered from clinical specimens.This study suggested that Shewanella strains can colonize and survive in human intestines.The study also raises the issues of the accumulation of TTX produced by Shewanella in food and the possible role of TTX-producing Shewanella in food poisoning.

View Article: PubMed Central - HTML - PubMed

Affiliation: State Key Laboratory for Infectious Disease Prevention and Control/Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, P,O, Box 5, Beijing, Changping, China. kanbiao@icdc.cn.

ABSTRACT
Shewanella spp. is infrequently recovered from clinical specimens. Following two outbreaks of food poisoning, eight Shewanella spp. strains were obtained from the fecal specimens of patients, food and food processing-related materials. Tetrodotoxin (TTX) was identified in the culture supernatants of these strains, and the toxin's biological activity was detected using a mouse bioassay. This study suggested that Shewanella strains can colonize and survive in human intestines. The study also raises the issues of the accumulation of TTX produced by Shewanella in food and the possible role of TTX-producing Shewanella in food poisoning.

No MeSH data available.


Related in: MedlinePlus

Mass Spectrometry analysis of the TTX extracts from the Shewanella isolates. A and B show the mass spectra of the sample containing TTX and of authentic TTX with ESI full MS. The retention time (RT) of the chromatogram of the sample, with a selective ion mode (SIM) specific for m/z 320 ions, was 2.45 min, which was similar to the RT (2.43 min) of authentic TTX (C and D). E and F show the MS/MS spectra of the sample containing TTX and of authentic TTX.
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Figure 2: Mass Spectrometry analysis of the TTX extracts from the Shewanella isolates. A and B show the mass spectra of the sample containing TTX and of authentic TTX with ESI full MS. The retention time (RT) of the chromatogram of the sample, with a selective ion mode (SIM) specific for m/z 320 ions, was 2.45 min, which was similar to the RT (2.43 min) of authentic TTX (C and D). E and F show the MS/MS spectra of the sample containing TTX and of authentic TTX.

Mentions: Because these patients were food poisoned and because TTX production has been reported for Shewanella strains [8], we measured TTX production by these isolates. TTX was detected from the seven-day culture supernatants by mass spectrometry assays (Figure 2). TTX extracts from cells and supernatants of the Shewanella spp. isolates were further subjected to an activity bioassay in mice (Table 1). After injection, TTX extracts from bacterial overnight cell cultures and supernatants and from seven-day cell cultures of all isolates did not induce symptoms of poisoning in the mice. In contrast, injection with TTX extracts from supernatants of seven-day cultures of all isolates caused the mice to show the same symptoms as those induced by a TTX standard in 0.5-13 minutes, including gait disorders, hindlimb weakness, convulsion and death. The mice in the control group had no obvious symptoms following the injection of 0.1% acetic acid.


Identification of tetrodotoxin-producing Shewanella spp. from feces of food poisoning patients and food samples.

Wang D, Wang Y, Huang H, Lin J, Xiao D, Kan B - Gut Pathog (2013)

Mass Spectrometry analysis of the TTX extracts from the Shewanella isolates. A and B show the mass spectra of the sample containing TTX and of authentic TTX with ESI full MS. The retention time (RT) of the chromatogram of the sample, with a selective ion mode (SIM) specific for m/z 320 ions, was 2.45 min, which was similar to the RT (2.43 min) of authentic TTX (C and D). E and F show the MS/MS spectra of the sample containing TTX and of authentic TTX.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3734009&req=5

Figure 2: Mass Spectrometry analysis of the TTX extracts from the Shewanella isolates. A and B show the mass spectra of the sample containing TTX and of authentic TTX with ESI full MS. The retention time (RT) of the chromatogram of the sample, with a selective ion mode (SIM) specific for m/z 320 ions, was 2.45 min, which was similar to the RT (2.43 min) of authentic TTX (C and D). E and F show the MS/MS spectra of the sample containing TTX and of authentic TTX.
Mentions: Because these patients were food poisoned and because TTX production has been reported for Shewanella strains [8], we measured TTX production by these isolates. TTX was detected from the seven-day culture supernatants by mass spectrometry assays (Figure 2). TTX extracts from cells and supernatants of the Shewanella spp. isolates were further subjected to an activity bioassay in mice (Table 1). After injection, TTX extracts from bacterial overnight cell cultures and supernatants and from seven-day cell cultures of all isolates did not induce symptoms of poisoning in the mice. In contrast, injection with TTX extracts from supernatants of seven-day cultures of all isolates caused the mice to show the same symptoms as those induced by a TTX standard in 0.5-13 minutes, including gait disorders, hindlimb weakness, convulsion and death. The mice in the control group had no obvious symptoms following the injection of 0.1% acetic acid.

Bottom Line: Shewanella spp. is infrequently recovered from clinical specimens.This study suggested that Shewanella strains can colonize and survive in human intestines.The study also raises the issues of the accumulation of TTX produced by Shewanella in food and the possible role of TTX-producing Shewanella in food poisoning.

View Article: PubMed Central - HTML - PubMed

Affiliation: State Key Laboratory for Infectious Disease Prevention and Control/Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, P,O, Box 5, Beijing, Changping, China. kanbiao@icdc.cn.

ABSTRACT
Shewanella spp. is infrequently recovered from clinical specimens. Following two outbreaks of food poisoning, eight Shewanella spp. strains were obtained from the fecal specimens of patients, food and food processing-related materials. Tetrodotoxin (TTX) was identified in the culture supernatants of these strains, and the toxin's biological activity was detected using a mouse bioassay. This study suggested that Shewanella strains can colonize and survive in human intestines. The study also raises the issues of the accumulation of TTX produced by Shewanella in food and the possible role of TTX-producing Shewanella in food poisoning.

No MeSH data available.


Related in: MedlinePlus