Limits...
Surveillance of the prevalence, antibiotic susceptibility, and genotypic characterization of invasive candidiasis in a teaching hospital in China between 2006 to 2011.

Li F, Wu L, Cao B, Zhang Y, Li X, Liu Y - BMC Infect. Dis. (2013)

Bottom Line: For the 136 C. albicans isolates, 54 CAI patterns were recognized.Resistance to fluconazole was highly predictive of resistance to voriconazole.CAI SSCP genotyping showed that all C. albicans strains were polymorphic.

View Article: PubMed Central - HTML - PubMed

ABSTRACT

Background: Invasive candidiasis is an important nosocomial infection associated with high mortality among immunosuppressive or critically ill patients. We described the incidence of invasive candidiasis in our hospital over 6 years and showed the antifungal susceptibility and genotypes of the isolated yeast.

Method: The yeast species were isolated on CHROMagar Candida medium and identified using an yeast identification card, followed by analysis of the D1/D2 domain of 26S rDNA. The susceptibilities of the isolates to flucytosine, amphotericin B, fluconazole, itraconazole, and voriconazole were tested using the ATB FUNGUS 3 system, and that to caspofungin was tested using E-test strips. C. albicans was genotyped using single-strand conformation polymorphism of CAI (Candida albicans I) microsatellite DNA combined with GeneScan data.

Results: From January 2006 to December 2011, a total of 259 isolates of invasive Candida spp. were obtained from 253 patients, among them 6 patients had multiple positive samples. Ninety-one stains were from blood and 168 from sterile fluids, accounting for 6.07% of all pathogens isolated in our hospital. Most of these strains were C. albicans (41.29% in blood/59.06% in sterile body fluids), followed by C. tropicalis (18.06%/25.72%), C. parapsilosis (17.42%/5.43%), C. glabrata (11.61%/3.99%) and other Candida spp. (11.61%/5.80%). Most Candida spp. were isolated from the ICU. The new species-specific CLSI candida MIC breakpoints were applied to these date. Resistance to fluconazole occurred in 6.6% of C. albicans isolates, 10.6% of C. tropicalis isolates and 15.0% of C. glabrata isolates. For the 136 C. albicans isolates, 54 CAI patterns were recognized. The C. albicans strains from blood or sterile body fluids showed no predominant CAI genotypes. C. albicans isolates from different samples from the same patient had the same genotype.

Conclusions: Invasive candidiasis has been commonly encountered in our hospital in the past 6 years, with increasing frequency of non-C. albicans. Resistance to fluconazole was highly predictive of resistance to voriconazole. CAI SSCP genotyping showed that all C. albicans strains were polymorphic. Invasive candidiasis were commonly endogenous infection.

Show MeSH

Related in: MedlinePlus

Changes in the isolation rate of Candida spp. in the last 6 years. The annual rates of invasive candidiasis from 2006 to 2011 were showed in the figure, according to the identification of Candida strains from bloodstream and sterile fluids (ascitic fluid, bile, central venous catheter and pleural fluid).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC3733982&req=5

Figure 1: Changes in the isolation rate of Candida spp. in the last 6 years. The annual rates of invasive candidiasis from 2006 to 2011 were showed in the figure, according to the identification of Candida strains from bloodstream and sterile fluids (ascitic fluid, bile, central venous catheter and pleural fluid).

Mentions: A total of 91 Candida strains were isolated from blood, which account for 5.75% of the 1583 pathogens isolated from blood from January 2006 to December 2011. The annual rates of candidemia over the 6 years of the study were as follows: 8.8%, 7.9%, 4.8%, 5.9%, 4.7%, and 2.4%. Further, 168 Candida strains were collected from sterile fluids (ascitic fluid, bile, central venous catheter and pleural fluid), which accounted for 6.27% of the 2684 pathogens isolated from sterile fluids (Figure 1). The distribution of these 259 invasive candidiasis specimens was as follows: blood 35.14%, ascitic fluid 35.03%, bile 17.53%, central venous catheter fluid 6.50%, and pleural fluids 5.80% (Figure 2). Most of these strains were C. albicans (41.29% in blood/59.06% in sterile body fluids), followed by C. tropicalis (18.06%/25.72%), C. parapsilosis (17.42%/5.43%), C. glabrata (11.61%/3.99%) and other Candida spp. (11.61%/5.80%) (Figure 3).


Surveillance of the prevalence, antibiotic susceptibility, and genotypic characterization of invasive candidiasis in a teaching hospital in China between 2006 to 2011.

Li F, Wu L, Cao B, Zhang Y, Li X, Liu Y - BMC Infect. Dis. (2013)

Changes in the isolation rate of Candida spp. in the last 6 years. The annual rates of invasive candidiasis from 2006 to 2011 were showed in the figure, according to the identification of Candida strains from bloodstream and sterile fluids (ascitic fluid, bile, central venous catheter and pleural fluid).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3733982&req=5

Figure 1: Changes in the isolation rate of Candida spp. in the last 6 years. The annual rates of invasive candidiasis from 2006 to 2011 were showed in the figure, according to the identification of Candida strains from bloodstream and sterile fluids (ascitic fluid, bile, central venous catheter and pleural fluid).
Mentions: A total of 91 Candida strains were isolated from blood, which account for 5.75% of the 1583 pathogens isolated from blood from January 2006 to December 2011. The annual rates of candidemia over the 6 years of the study were as follows: 8.8%, 7.9%, 4.8%, 5.9%, 4.7%, and 2.4%. Further, 168 Candida strains were collected from sterile fluids (ascitic fluid, bile, central venous catheter and pleural fluid), which accounted for 6.27% of the 2684 pathogens isolated from sterile fluids (Figure 1). The distribution of these 259 invasive candidiasis specimens was as follows: blood 35.14%, ascitic fluid 35.03%, bile 17.53%, central venous catheter fluid 6.50%, and pleural fluids 5.80% (Figure 2). Most of these strains were C. albicans (41.29% in blood/59.06% in sterile body fluids), followed by C. tropicalis (18.06%/25.72%), C. parapsilosis (17.42%/5.43%), C. glabrata (11.61%/3.99%) and other Candida spp. (11.61%/5.80%) (Figure 3).

Bottom Line: For the 136 C. albicans isolates, 54 CAI patterns were recognized.Resistance to fluconazole was highly predictive of resistance to voriconazole.CAI SSCP genotyping showed that all C. albicans strains were polymorphic.

View Article: PubMed Central - HTML - PubMed

ABSTRACT

Background: Invasive candidiasis is an important nosocomial infection associated with high mortality among immunosuppressive or critically ill patients. We described the incidence of invasive candidiasis in our hospital over 6 years and showed the antifungal susceptibility and genotypes of the isolated yeast.

Method: The yeast species were isolated on CHROMagar Candida medium and identified using an yeast identification card, followed by analysis of the D1/D2 domain of 26S rDNA. The susceptibilities of the isolates to flucytosine, amphotericin B, fluconazole, itraconazole, and voriconazole were tested using the ATB FUNGUS 3 system, and that to caspofungin was tested using E-test strips. C. albicans was genotyped using single-strand conformation polymorphism of CAI (Candida albicans I) microsatellite DNA combined with GeneScan data.

Results: From January 2006 to December 2011, a total of 259 isolates of invasive Candida spp. were obtained from 253 patients, among them 6 patients had multiple positive samples. Ninety-one stains were from blood and 168 from sterile fluids, accounting for 6.07% of all pathogens isolated in our hospital. Most of these strains were C. albicans (41.29% in blood/59.06% in sterile body fluids), followed by C. tropicalis (18.06%/25.72%), C. parapsilosis (17.42%/5.43%), C. glabrata (11.61%/3.99%) and other Candida spp. (11.61%/5.80%). Most Candida spp. were isolated from the ICU. The new species-specific CLSI candida MIC breakpoints were applied to these date. Resistance to fluconazole occurred in 6.6% of C. albicans isolates, 10.6% of C. tropicalis isolates and 15.0% of C. glabrata isolates. For the 136 C. albicans isolates, 54 CAI patterns were recognized. The C. albicans strains from blood or sterile body fluids showed no predominant CAI genotypes. C. albicans isolates from different samples from the same patient had the same genotype.

Conclusions: Invasive candidiasis has been commonly encountered in our hospital in the past 6 years, with increasing frequency of non-C. albicans. Resistance to fluconazole was highly predictive of resistance to voriconazole. CAI SSCP genotyping showed that all C. albicans strains were polymorphic. Invasive candidiasis were commonly endogenous infection.

Show MeSH
Related in: MedlinePlus