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Quantitative analysis of the major constituents in Chinese medicinal preparation SuoQuan formulae by ultra fast high performance liquid chromatography/quadrupole tandem mass spectrometry.

Chen F, Li HL, Li YH, Tan YF, Zhang JQ - Chem Cent J (2013)

Bottom Line: This method was successfully applied to simultaneously quantify the fourteen phytochemicals.Notably, the content of these constituents showed significant differences in three pharmaceutical preparations.Furthermore, the significant content variation between the two different Suoquan pills was also observed.

View Article: PubMed Central - HTML - PubMed

Affiliation: School of Pharmacy, Hainan Medical University, Hainan Provincial Key Laboratory of R&D of Tropical Herbs, Haikou 571101, China. cy.chen508@gmail.com.

ABSTRACT

Background: The SuoQuan formulae containing Fructus Alpiniae Oxyphyllae has been used to combat the urinary incontinence symptoms including frequency, urgency and nocturia for hundreds of years in China. However, the chemical information was not well characterized. The quality control marker constituent only focused on one single compound in the current Chinese Pharmacopeia. Hence it is prudent to identify and quantify the main constituents in this herbal product. This study aimed to analyze the main constituents using ultra-fast performance liquid chromatography coupled to tandem mass spectrometry (UFLC-MS/MS).

Results: Fourteen phytochemicals originated from five chemical classes constituents were identified by comparing the molecular mass, fragmentation pattern and retention time with those of the reference standards. A newly developed UFLC-MS/MS was validated demonstrating that the new assay was valid, reproducible and reliable. This method was successfully applied to simultaneously quantify the fourteen phytochemicals. Notably, the content of these constituents showed significant differences in three pharmaceutical preparations. The major constituent originated from each of chemical class was isolinderalactone, norisoboldine, nootkatone, yakuchinone A and apigenin-4',7-dimethylther, respectively. The variation among these compounds was more than 1000 times. Furthermore, the significant content variation between the two different Suoquan pills was also observed.

Conclusion: The proposed method is sensitive and reliable; hence it can be used to analyze a variety of SuoQuan formulae products produced by different pharmaceutical manufacturers.

No MeSH data available.


Related in: MedlinePlus

The content variation of 14 phytochemicals originated from SQC, SQP-1 and SQP-2 samples. In the bottom panel, the content level variation was normalized based on the contents in SQP-1 sample.
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Figure 3: The content variation of 14 phytochemicals originated from SQC, SQP-1 and SQP-2 samples. In the bottom panel, the content level variation was normalized based on the contents in SQP-1 sample.

Mentions: The newly validated method was applied to simultaneously determine the 14 compounds in three commercial SuoQuan formulae products from different company. The results are summarized in Table 5 and Figure 3. Our results revealed that the contents of the 14 compounds are significantly different. The overall content level was as follows: lactones > alkaloids > naphthalenone (nootkatone) > diarylheptanoids > flavonoids, albeit the dosage forms (i.e., capsules or pills). Accordingly, the highest content constituent of each type of compound was isolinderalactone, norisoboldine, nootkatone, yakuchinone A and apigenin-4’,7-dimethylther, respectively. Moreover, the content variation was more than 1500 times among these phytochemicals .


Quantitative analysis of the major constituents in Chinese medicinal preparation SuoQuan formulae by ultra fast high performance liquid chromatography/quadrupole tandem mass spectrometry.

Chen F, Li HL, Li YH, Tan YF, Zhang JQ - Chem Cent J (2013)

The content variation of 14 phytochemicals originated from SQC, SQP-1 and SQP-2 samples. In the bottom panel, the content level variation was normalized based on the contents in SQP-1 sample.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3733971&req=5

Figure 3: The content variation of 14 phytochemicals originated from SQC, SQP-1 and SQP-2 samples. In the bottom panel, the content level variation was normalized based on the contents in SQP-1 sample.
Mentions: The newly validated method was applied to simultaneously determine the 14 compounds in three commercial SuoQuan formulae products from different company. The results are summarized in Table 5 and Figure 3. Our results revealed that the contents of the 14 compounds are significantly different. The overall content level was as follows: lactones > alkaloids > naphthalenone (nootkatone) > diarylheptanoids > flavonoids, albeit the dosage forms (i.e., capsules or pills). Accordingly, the highest content constituent of each type of compound was isolinderalactone, norisoboldine, nootkatone, yakuchinone A and apigenin-4’,7-dimethylther, respectively. Moreover, the content variation was more than 1500 times among these phytochemicals .

Bottom Line: This method was successfully applied to simultaneously quantify the fourteen phytochemicals.Notably, the content of these constituents showed significant differences in three pharmaceutical preparations.Furthermore, the significant content variation between the two different Suoquan pills was also observed.

View Article: PubMed Central - HTML - PubMed

Affiliation: School of Pharmacy, Hainan Medical University, Hainan Provincial Key Laboratory of R&D of Tropical Herbs, Haikou 571101, China. cy.chen508@gmail.com.

ABSTRACT

Background: The SuoQuan formulae containing Fructus Alpiniae Oxyphyllae has been used to combat the urinary incontinence symptoms including frequency, urgency and nocturia for hundreds of years in China. However, the chemical information was not well characterized. The quality control marker constituent only focused on one single compound in the current Chinese Pharmacopeia. Hence it is prudent to identify and quantify the main constituents in this herbal product. This study aimed to analyze the main constituents using ultra-fast performance liquid chromatography coupled to tandem mass spectrometry (UFLC-MS/MS).

Results: Fourteen phytochemicals originated from five chemical classes constituents were identified by comparing the molecular mass, fragmentation pattern and retention time with those of the reference standards. A newly developed UFLC-MS/MS was validated demonstrating that the new assay was valid, reproducible and reliable. This method was successfully applied to simultaneously quantify the fourteen phytochemicals. Notably, the content of these constituents showed significant differences in three pharmaceutical preparations. The major constituent originated from each of chemical class was isolinderalactone, norisoboldine, nootkatone, yakuchinone A and apigenin-4',7-dimethylther, respectively. The variation among these compounds was more than 1000 times. Furthermore, the significant content variation between the two different Suoquan pills was also observed.

Conclusion: The proposed method is sensitive and reliable; hence it can be used to analyze a variety of SuoQuan formulae products produced by different pharmaceutical manufacturers.

No MeSH data available.


Related in: MedlinePlus