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Study of 27 aqueous humor cytokines in patients with type 2 diabetes with or without retinopathy.

Dong N, Xu B, Wang B, Chu L - Mol. Vis. (2013)

Bottom Line: There were no significant differences in the concentrations of other cytokines between the diabetic patients and the controls.The correlation was significant.Various cytokines associated with inflammation and angiogenesis may contribute to the pathogenesis of DR, and chemokines may be more closely related to the development of this disease.

View Article: PubMed Central - PubMed

Affiliation: Department of Ophthalmology, Beijing Shijitan Hospital, Capital Medical University, Beijing, People's Republic of China.

ABSTRACT

Purpose: To compare the changes in the levels of 27 aqueous humor cytokines between nondiabetic controls and patients with type 2 diabetes and to ascertain the association of these cytokines with diabetic retinopathy (DR).

Methods: Undiluted aqueous humor samples were obtained from 102 nondiabetic patients (102 eyes) and 136 consecutive diabetic patients (136 eyes) who were divided into nine groups according to the Early Treatment of Diabetic Retinopathy Study severity scale. The concentrations of 27 cytokines in the aqueous humor samples were measured using a multiplex bead immunoassay.

Results: Compared with the nondiabetic controls, the diabetic patients had significantly higher concentrations of interleukin-1β (IL-1β; p<0.001), IL-6 (p<0.001), IL-8 (p<0.001), monocyte chemoattractant protein-1 (p<0.001), interferon gamma-induced protein-10 (p<0.001), and vascular endothelial growth factor (p<0.001) in the aqueous humor. However, the IL-10 (p=0.002) and IL-12 (p=0.013) concentrations were significantly lower for the diabetic patients. There were no significant differences in the concentrations of other cytokines between the diabetic patients and the controls. The IL-1β, IL-6, IL-8, monocyte chemoattractant protein-1, and interferon gamma-induced protein-10 levels in the aqueous humor increased as the severity of DR increased. The correlation was significant. However, the vascular endothelial growth factor concentration was not correlated with the severity of DR. In addition, the IL-10 and IL-12 levels in the aqueous humor decreased as the severity of DR increased, and this negative correlation was significant.

Conclusions: Various cytokines associated with inflammation and angiogenesis may contribute to the pathogenesis of DR, and chemokines may be more closely related to the development of this disease.

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Related in: MedlinePlus

Relationship between the severity of diabetic retinopathy (DR) and the aqueous vascular endothelial growth factor (VEGF) level. The results by measuring cytokines in the aqueous humor samples shows that the VEGF level in aqueous humor did not increase with increasing severity of DR, and this correlation was not significant (r=0.161, p=0.062). Table 3 shows the sampling sizes for each group according to the ETDRS retinopathy severity scale.
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f6: Relationship between the severity of diabetic retinopathy (DR) and the aqueous vascular endothelial growth factor (VEGF) level. The results by measuring cytokines in the aqueous humor samples shows that the VEGF level in aqueous humor did not increase with increasing severity of DR, and this correlation was not significant (r=0.161, p=0.062). Table 3 shows the sampling sizes for each group according to the ETDRS retinopathy severity scale.

Mentions: According to the ETDRS retinopathy severity scale, 28 eyes were level 10, 23 eyes were level 20, 26 were level 35, 18 eyes were level 43, 13 eyes were level 47, eight eyes were level 53, seven eyes were level 65, eight eyes were level 75, and five eyes were level 81. Table 3 and Table 4 and Figure 1, Figure 2, Figure 3, Figure 4, Figure 5, Figure 6, Figure 7, and Figure 8 show the relationship between the concentrations of the assayed cytokines and DR severity. The aqueous humor levels of IL-1β, IL-6, IL-8, MCP-1, and IP-10 increased as DR increased; this correlation was significant. However, the concentration of VEGF was not correlated with DR severity. In addition, the aqueous humor levels of IL-10 and IL-12 decreased as DR severity increased; this negative correlation was significant.


Study of 27 aqueous humor cytokines in patients with type 2 diabetes with or without retinopathy.

Dong N, Xu B, Wang B, Chu L - Mol. Vis. (2013)

Relationship between the severity of diabetic retinopathy (DR) and the aqueous vascular endothelial growth factor (VEGF) level. The results by measuring cytokines in the aqueous humor samples shows that the VEGF level in aqueous humor did not increase with increasing severity of DR, and this correlation was not significant (r=0.161, p=0.062). Table 3 shows the sampling sizes for each group according to the ETDRS retinopathy severity scale.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3733907&req=5

f6: Relationship between the severity of diabetic retinopathy (DR) and the aqueous vascular endothelial growth factor (VEGF) level. The results by measuring cytokines in the aqueous humor samples shows that the VEGF level in aqueous humor did not increase with increasing severity of DR, and this correlation was not significant (r=0.161, p=0.062). Table 3 shows the sampling sizes for each group according to the ETDRS retinopathy severity scale.
Mentions: According to the ETDRS retinopathy severity scale, 28 eyes were level 10, 23 eyes were level 20, 26 were level 35, 18 eyes were level 43, 13 eyes were level 47, eight eyes were level 53, seven eyes were level 65, eight eyes were level 75, and five eyes were level 81. Table 3 and Table 4 and Figure 1, Figure 2, Figure 3, Figure 4, Figure 5, Figure 6, Figure 7, and Figure 8 show the relationship between the concentrations of the assayed cytokines and DR severity. The aqueous humor levels of IL-1β, IL-6, IL-8, MCP-1, and IP-10 increased as DR increased; this correlation was significant. However, the concentration of VEGF was not correlated with DR severity. In addition, the aqueous humor levels of IL-10 and IL-12 decreased as DR severity increased; this negative correlation was significant.

Bottom Line: There were no significant differences in the concentrations of other cytokines between the diabetic patients and the controls.The correlation was significant.Various cytokines associated with inflammation and angiogenesis may contribute to the pathogenesis of DR, and chemokines may be more closely related to the development of this disease.

View Article: PubMed Central - PubMed

Affiliation: Department of Ophthalmology, Beijing Shijitan Hospital, Capital Medical University, Beijing, People's Republic of China.

ABSTRACT

Purpose: To compare the changes in the levels of 27 aqueous humor cytokines between nondiabetic controls and patients with type 2 diabetes and to ascertain the association of these cytokines with diabetic retinopathy (DR).

Methods: Undiluted aqueous humor samples were obtained from 102 nondiabetic patients (102 eyes) and 136 consecutive diabetic patients (136 eyes) who were divided into nine groups according to the Early Treatment of Diabetic Retinopathy Study severity scale. The concentrations of 27 cytokines in the aqueous humor samples were measured using a multiplex bead immunoassay.

Results: Compared with the nondiabetic controls, the diabetic patients had significantly higher concentrations of interleukin-1β (IL-1β; p<0.001), IL-6 (p<0.001), IL-8 (p<0.001), monocyte chemoattractant protein-1 (p<0.001), interferon gamma-induced protein-10 (p<0.001), and vascular endothelial growth factor (p<0.001) in the aqueous humor. However, the IL-10 (p=0.002) and IL-12 (p=0.013) concentrations were significantly lower for the diabetic patients. There were no significant differences in the concentrations of other cytokines between the diabetic patients and the controls. The IL-1β, IL-6, IL-8, monocyte chemoattractant protein-1, and interferon gamma-induced protein-10 levels in the aqueous humor increased as the severity of DR increased. The correlation was significant. However, the vascular endothelial growth factor concentration was not correlated with the severity of DR. In addition, the IL-10 and IL-12 levels in the aqueous humor decreased as the severity of DR increased, and this negative correlation was significant.

Conclusions: Various cytokines associated with inflammation and angiogenesis may contribute to the pathogenesis of DR, and chemokines may be more closely related to the development of this disease.

Show MeSH
Related in: MedlinePlus