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Serum IL-10 as a marker of severe dengue infection.

Malavige GN, Gomes L, Alles L, Chang T, Salimi M, Fernando S, Nanayakkara KD, Jayaratne S, Ogg GS - BMC Infect. Dis. (2013)

Bottom Line: We found that serum IL-10 levels were significantly higher (p = 0.001) in patients with SD, when compared to those with non SD.However, IL-10 levels did not have a good predictive value in discriminating those who were likely to develop SD (AUC = 0.66).Serum IFNγ levels were also significantly higher (p = 0.04) in patients with SD when compared to non SD.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Microbiology, University of Sri Jayawardanapura, Nugegoda, Sri Lanka. gathsaurie.malavige@ndm.ox.ac.uk

ABSTRACT

Background: Several studies have shown that serum IL-10, IFNγ and MIF are elevated in patients in severe dengue (SD) and could be used as potential biomarkers. We proceeded to determine if these cytokines could be used as biomarkers in a large cohort of adult dengue patients with varying severity of dengue infection.

Methods: Serum IL-10 levels were determined in 259 of whom 40 had severe dengue infection. Serum IFNγ and IFNα levels were done in 78 and MIF levels were done in 65 patients with acute dengue infection. Clinical features and laboratory investigations were undertaken during the febrile and critical phase.

Results: We found that serum IL-10 levels were significantly higher (p = 0.001) in patients with SD, when compared to those with non SD. Serum IL-10 levels significantly and inversely correlated with white cell counts (R = -0.23, p = 0.0002) and lymphocyte counts (R = -0.29, p < 0.0001) but significantly and positively correlated with aspartate tranaminase levels (R = 0.16, p = 0.01) and alanine transaminase levels (R = 0.22, p = 0.007). However, IL-10 levels did not have a good predictive value in discriminating those who were likely to develop SD (AUC = 0.66). Serum IFNγ levels were also significantly higher (p = 0.04) in patients with SD when compared to non SD. There was no difference (p = 0.34) in serum IFNα levels and serum MIF levels (p = 0.15) in patients with SD and non SD.

Conclusion: Although serum IL-10 was significantly elevated in patients with SD it had a poor discriminatory value in identifying those with SD and non SD and therefore, is unsuitable to be used as a robust biomarker in this cohort.

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Related in: MedlinePlus

Serum IL-10 levels and clinical diseases severity. A: Serum IL-10 levels in patients with severe dengue (n = 40), non severe dengue (n = 219) and healthy controls (n = 11). B: Serum IL-10 levels in patients with severe dengue (SD) (n = 7) and non severe dengue (n = 57) in the febrile phase and the critical phase. C: ROC curves of serum IL-10 levels (n-259).
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Figure 1: Serum IL-10 levels and clinical diseases severity. A: Serum IL-10 levels in patients with severe dengue (n = 40), non severe dengue (n = 219) and healthy controls (n = 11). B: Serum IL-10 levels in patients with severe dengue (SD) (n = 7) and non severe dengue (n = 57) in the febrile phase and the critical phase. C: ROC curves of serum IL-10 levels (n-259).

Mentions: Patients with severe dengue had significantly lower white cell counts and platelet counts and significantly higher alanine transaminase levels and aspartate transaminase levels than those with non severe dengue (Table 1). Serum IL-10 levels were significantly higher (p = 0.001) in patients with severe dengue (mean 291.6 ± SD 573.6, median 154.3, range 24.98 to 3271 pg/ml), when compared to those with non severe dengue (mean 156.6 ± SD 258.2, median 81.9, range 7.8 to 2681 pg/ml) (Figure 1A). Serum IL-10 levels significantly and inversely correlated with white cell counts (Spearmans R = −0.23, p = 0.0002) and lymphocyte counts (Spearmans R = −0.29, p < 0.0001). Serum IL-10 levels significantly and positively correlated with the highest aspartate tranaminase (AST) levels (Spearmans R = 0.16, p = 0.01) and the highest alanine transaminase (ALT) levels (Spearmans R = 0.22, p = 0.007) but not with the lowest platelet count in the critical phase (Table 2).


Serum IL-10 as a marker of severe dengue infection.

Malavige GN, Gomes L, Alles L, Chang T, Salimi M, Fernando S, Nanayakkara KD, Jayaratne S, Ogg GS - BMC Infect. Dis. (2013)

Serum IL-10 levels and clinical diseases severity. A: Serum IL-10 levels in patients with severe dengue (n = 40), non severe dengue (n = 219) and healthy controls (n = 11). B: Serum IL-10 levels in patients with severe dengue (SD) (n = 7) and non severe dengue (n = 57) in the febrile phase and the critical phase. C: ROC curves of serum IL-10 levels (n-259).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3733887&req=5

Figure 1: Serum IL-10 levels and clinical diseases severity. A: Serum IL-10 levels in patients with severe dengue (n = 40), non severe dengue (n = 219) and healthy controls (n = 11). B: Serum IL-10 levels in patients with severe dengue (SD) (n = 7) and non severe dengue (n = 57) in the febrile phase and the critical phase. C: ROC curves of serum IL-10 levels (n-259).
Mentions: Patients with severe dengue had significantly lower white cell counts and platelet counts and significantly higher alanine transaminase levels and aspartate transaminase levels than those with non severe dengue (Table 1). Serum IL-10 levels were significantly higher (p = 0.001) in patients with severe dengue (mean 291.6 ± SD 573.6, median 154.3, range 24.98 to 3271 pg/ml), when compared to those with non severe dengue (mean 156.6 ± SD 258.2, median 81.9, range 7.8 to 2681 pg/ml) (Figure 1A). Serum IL-10 levels significantly and inversely correlated with white cell counts (Spearmans R = −0.23, p = 0.0002) and lymphocyte counts (Spearmans R = −0.29, p < 0.0001). Serum IL-10 levels significantly and positively correlated with the highest aspartate tranaminase (AST) levels (Spearmans R = 0.16, p = 0.01) and the highest alanine transaminase (ALT) levels (Spearmans R = 0.22, p = 0.007) but not with the lowest platelet count in the critical phase (Table 2).

Bottom Line: We found that serum IL-10 levels were significantly higher (p = 0.001) in patients with SD, when compared to those with non SD.However, IL-10 levels did not have a good predictive value in discriminating those who were likely to develop SD (AUC = 0.66).Serum IFNγ levels were also significantly higher (p = 0.04) in patients with SD when compared to non SD.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Microbiology, University of Sri Jayawardanapura, Nugegoda, Sri Lanka. gathsaurie.malavige@ndm.ox.ac.uk

ABSTRACT

Background: Several studies have shown that serum IL-10, IFNγ and MIF are elevated in patients in severe dengue (SD) and could be used as potential biomarkers. We proceeded to determine if these cytokines could be used as biomarkers in a large cohort of adult dengue patients with varying severity of dengue infection.

Methods: Serum IL-10 levels were determined in 259 of whom 40 had severe dengue infection. Serum IFNγ and IFNα levels were done in 78 and MIF levels were done in 65 patients with acute dengue infection. Clinical features and laboratory investigations were undertaken during the febrile and critical phase.

Results: We found that serum IL-10 levels were significantly higher (p = 0.001) in patients with SD, when compared to those with non SD. Serum IL-10 levels significantly and inversely correlated with white cell counts (R = -0.23, p = 0.0002) and lymphocyte counts (R = -0.29, p < 0.0001) but significantly and positively correlated with aspartate tranaminase levels (R = 0.16, p = 0.01) and alanine transaminase levels (R = 0.22, p = 0.007). However, IL-10 levels did not have a good predictive value in discriminating those who were likely to develop SD (AUC = 0.66). Serum IFNγ levels were also significantly higher (p = 0.04) in patients with SD when compared to non SD. There was no difference (p = 0.34) in serum IFNα levels and serum MIF levels (p = 0.15) in patients with SD and non SD.

Conclusion: Although serum IL-10 was significantly elevated in patients with SD it had a poor discriminatory value in identifying those with SD and non SD and therefore, is unsuitable to be used as a robust biomarker in this cohort.

Show MeSH
Related in: MedlinePlus