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The impact of high fat diets on physiological changes in euthyroid and thyroid altered rats.

Welch-White V, Dawkins N, Graham T, Pace R - Lipids Health Dis (2013)

Bottom Line: HbA1C values and urinary glucose values were within normal range for all animals.Liver morphology showed increased hepatic stellate (ito) and vacuole cells in thyroid altered animals.These findings suggest that altered thyroid status negatively impacts growth and weight gain, and simultaneously affected lipid metabolism, resulting in abnormal liver morphology.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Food and Nutritional Sciences, Tuskegee University, 204 Campbell Hall, Tuskegee, AL 36088, USA.

ABSTRACT
The association of adverse health with high fat intake has long been recognized. However, the lack of research focusing on the interrelationship of thyroid and liver function, and the pathogenesis of a high fat diet leaves these topics poorly understood. The objective of this study was to evaluate and compare the physiological changes in euthyroid and thyroid altered animal model fed saturated and unsaturated high fat diets. To achieve this objective adult male Sprague Dawley rats (n = 100) were fed one of five diets; a control or one of four test diets containing 25% saturated or unsaturated, and 37% saturated or unsaturated fats for a period of eight weeks. Each experimental group consisted of ten euthyroid and ten thyroid altered animals. An altered thyroid state was chemically induced with the addition of 0.05% propylthiouracil (PTU) in the drinking water. Euthyroid animals fed high fat diets increased in body weights and body lengths, compared to thyroid altered animals (P < 0.05). Alanine aminotransferase (ALT) and asparte aminotransferase (AST) levels increased across all experimental groups. HbA1C values and urinary glucose values were within normal range for all animals. Liver morphology showed increased hepatic stellate (ito) and vacuole cells in thyroid altered animals. These findings suggest that altered thyroid status negatively impacts growth and weight gain, and simultaneously affected lipid metabolism, resulting in abnormal liver morphology.

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Histopathological evaluation of liver in Rats fed Lard Based Diets. (A) Represents a normal liver with normal morphology from a euthyroid animal which consumed a control diet. (B) Represents a normal liver with normal morphology from a thyroid altered animal which consumed a control diet. (C) Represents a normal liver in a euthyroid animal with normal morphology, which consumed a 25% lard diet. (D) Shows liver from a thyroid altered animal which consumed 25% lard diet. There is evidence of +1 ito cells and a hepatocyte with the presence of two small cytoplasmic vacuoles. (E) Represents the liver from a euthyroid animal which consumed 37% lard diet that shows the presence of 3 ito cells (+4) and multiple vacuoles (+3). (F) Represents the liver from a thyroid altered animal which consumed 37% lard diet that shows the Presence of ito cells (+4) and vacuoles (+2).
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Figure 1: Histopathological evaluation of liver in Rats fed Lard Based Diets. (A) Represents a normal liver with normal morphology from a euthyroid animal which consumed a control diet. (B) Represents a normal liver with normal morphology from a thyroid altered animal which consumed a control diet. (C) Represents a normal liver in a euthyroid animal with normal morphology, which consumed a 25% lard diet. (D) Shows liver from a thyroid altered animal which consumed 25% lard diet. There is evidence of +1 ito cells and a hepatocyte with the presence of two small cytoplasmic vacuoles. (E) Represents the liver from a euthyroid animal which consumed 37% lard diet that shows the presence of 3 ito cells (+4) and multiple vacuoles (+3). (F) Represents the liver from a thyroid altered animal which consumed 37% lard diet that shows the Presence of ito cells (+4) and vacuoles (+2).

Mentions: The results of the histopathological evaluation of livers from rats are shown in Table 7. The tissues showed an increased presence of hepatic stellate (ito) cells and vacuoles in the thyroid altered animal compared to the euthyroid animals. The thyroid altered animals had an occurrence of vacuoles of 60% or greater across all treatment groups. Morphological changes were observed in the 37% fat dietary groups, as well as in the thyroid altered animals. Hepatic stellate (ito) cells are naturally occurring lipid containing cells and play a key role in the pathogenesis of hepatic fibrosis [35]. Fibrosis is the essential pathophysiologic consequence of chronic liver injury, and it represents the common underlying mechanism for hepatic insufficiency [36] Figure 1 and 2.


The impact of high fat diets on physiological changes in euthyroid and thyroid altered rats.

Welch-White V, Dawkins N, Graham T, Pace R - Lipids Health Dis (2013)

Histopathological evaluation of liver in Rats fed Lard Based Diets. (A) Represents a normal liver with normal morphology from a euthyroid animal which consumed a control diet. (B) Represents a normal liver with normal morphology from a thyroid altered animal which consumed a control diet. (C) Represents a normal liver in a euthyroid animal with normal morphology, which consumed a 25% lard diet. (D) Shows liver from a thyroid altered animal which consumed 25% lard diet. There is evidence of +1 ito cells and a hepatocyte with the presence of two small cytoplasmic vacuoles. (E) Represents the liver from a euthyroid animal which consumed 37% lard diet that shows the presence of 3 ito cells (+4) and multiple vacuoles (+3). (F) Represents the liver from a thyroid altered animal which consumed 37% lard diet that shows the Presence of ito cells (+4) and vacuoles (+2).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3733864&req=5

Figure 1: Histopathological evaluation of liver in Rats fed Lard Based Diets. (A) Represents a normal liver with normal morphology from a euthyroid animal which consumed a control diet. (B) Represents a normal liver with normal morphology from a thyroid altered animal which consumed a control diet. (C) Represents a normal liver in a euthyroid animal with normal morphology, which consumed a 25% lard diet. (D) Shows liver from a thyroid altered animal which consumed 25% lard diet. There is evidence of +1 ito cells and a hepatocyte with the presence of two small cytoplasmic vacuoles. (E) Represents the liver from a euthyroid animal which consumed 37% lard diet that shows the presence of 3 ito cells (+4) and multiple vacuoles (+3). (F) Represents the liver from a thyroid altered animal which consumed 37% lard diet that shows the Presence of ito cells (+4) and vacuoles (+2).
Mentions: The results of the histopathological evaluation of livers from rats are shown in Table 7. The tissues showed an increased presence of hepatic stellate (ito) cells and vacuoles in the thyroid altered animal compared to the euthyroid animals. The thyroid altered animals had an occurrence of vacuoles of 60% or greater across all treatment groups. Morphological changes were observed in the 37% fat dietary groups, as well as in the thyroid altered animals. Hepatic stellate (ito) cells are naturally occurring lipid containing cells and play a key role in the pathogenesis of hepatic fibrosis [35]. Fibrosis is the essential pathophysiologic consequence of chronic liver injury, and it represents the common underlying mechanism for hepatic insufficiency [36] Figure 1 and 2.

Bottom Line: HbA1C values and urinary glucose values were within normal range for all animals.Liver morphology showed increased hepatic stellate (ito) and vacuole cells in thyroid altered animals.These findings suggest that altered thyroid status negatively impacts growth and weight gain, and simultaneously affected lipid metabolism, resulting in abnormal liver morphology.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Food and Nutritional Sciences, Tuskegee University, 204 Campbell Hall, Tuskegee, AL 36088, USA.

ABSTRACT
The association of adverse health with high fat intake has long been recognized. However, the lack of research focusing on the interrelationship of thyroid and liver function, and the pathogenesis of a high fat diet leaves these topics poorly understood. The objective of this study was to evaluate and compare the physiological changes in euthyroid and thyroid altered animal model fed saturated and unsaturated high fat diets. To achieve this objective adult male Sprague Dawley rats (n = 100) were fed one of five diets; a control or one of four test diets containing 25% saturated or unsaturated, and 37% saturated or unsaturated fats for a period of eight weeks. Each experimental group consisted of ten euthyroid and ten thyroid altered animals. An altered thyroid state was chemically induced with the addition of 0.05% propylthiouracil (PTU) in the drinking water. Euthyroid animals fed high fat diets increased in body weights and body lengths, compared to thyroid altered animals (P < 0.05). Alanine aminotransferase (ALT) and asparte aminotransferase (AST) levels increased across all experimental groups. HbA1C values and urinary glucose values were within normal range for all animals. Liver morphology showed increased hepatic stellate (ito) and vacuole cells in thyroid altered animals. These findings suggest that altered thyroid status negatively impacts growth and weight gain, and simultaneously affected lipid metabolism, resulting in abnormal liver morphology.

Show MeSH
Related in: MedlinePlus