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Adequacy of ovarian diathermy under ultrasound control: an experimental model.

Pimentel AM, Kliemann LM, Brum Ddos S, Leivas FG, Sanches PR, Capp E, Corleta Hv - J Ovarian Res (2013)

Bottom Line: No lesions could be found in the needle path.The ovaries were successfully cauterized without injuries in needle path and more energy resulted in significantly more thermal lesion.The safety and effectiveness of this technique, theoretically less invasive than current ovarian drilling methods, could be tested in anovulatory women with PCOS.

View Article: PubMed Central - HTML - PubMed

Affiliation: Programa de Pós-Graduação em Medicina: Ciências Médicas, Hospital de Clínicas de Porto Alegre, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil.

ABSTRACT

Background: To develop a minimally invasive ovarian cauterization technique under transvaginal ultrasound control and evaluate the safety and feasability of monopolar cauterization to cause ovarian injury using female cattle of reproductive age as an experimental model.

Method: Experimental study in a university research center was performed. Eleven female bovines of reproductive age were submitted to monopolar transvaginal ovarian cauterization. The right ovary (RO) was punctured at four sites and 40 W was applied for 5 s at each point, resulting in a total of 800 J (Joules) of thermal energy. In the left ovary (LO), the procedure was similar, with the same time and 80 W, resulting in a thermal energy of 1600 J. Macroscopic and microscopic lesions were assessed.

Results: Of 22 ovaries punctured, 20 were cauterized and exhibited macroscopic and typical microscopic lesions. No lesions could be found in the needle path. The measures of the areas of microscopic electrocautery lesions calculated estimating a cylindrical volume showed a median of 1.12% in the right ovary and 1.65% in the left ovary. When the estimate was calculated by spherical shape, the medians were 1.77% in the right ovary and 3.06% in the left ovary. There was a statistically significant difference in these two estimates (sphere, p = 0.008; cylinder, p = 0.021).

Conclusion: The experimental animal model described for transvaginal ultrasound-guided ovarian needle cauterization seems to be feasible. The ovaries were successfully cauterized without injuries in needle path and more energy resulted in significantly more thermal lesion. The safety and effectiveness of this technique, theoretically less invasive than current ovarian drilling methods, could be tested in anovulatory women with PCOS.

No MeSH data available.


Related in: MedlinePlus

Box-and-whisker plot of mean ovarian tissue damage. Lesions estimated by cylinder or sphere volume. The upper line of the box represents the upper quartile, the thick line in the middle represents the median, and the lower line represents the lower quartile. Lesion volumes estimated by the two methods were statistically different between the two ovaries (*p = 0.021 and **p = 0.008).
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Figure 4: Box-and-whisker plot of mean ovarian tissue damage. Lesions estimated by cylinder or sphere volume. The upper line of the box represents the upper quartile, the thick line in the middle represents the median, and the lower line represents the lower quartile. Lesion volumes estimated by the two methods were statistically different between the two ovaries (*p = 0.021 and **p = 0.008).

Mentions: The measures of the lesioned areas calculated by cylindrical volume estimates showed a median of 1.12% in the right ovary and 1.65% in the left ovary. When estimation was calculated by spherical shape, the medians were 1.77% in the right ovary and 3.06% in the left ovary. There was a statistically significant difference in these two estimates of lesion area of the right ovaries (800 J) and left ovaries (1600 J) (p <0.05), as shown in Figure 4.


Adequacy of ovarian diathermy under ultrasound control: an experimental model.

Pimentel AM, Kliemann LM, Brum Ddos S, Leivas FG, Sanches PR, Capp E, Corleta Hv - J Ovarian Res (2013)

Box-and-whisker plot of mean ovarian tissue damage. Lesions estimated by cylinder or sphere volume. The upper line of the box represents the upper quartile, the thick line in the middle represents the median, and the lower line represents the lower quartile. Lesion volumes estimated by the two methods were statistically different between the two ovaries (*p = 0.021 and **p = 0.008).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3733766&req=5

Figure 4: Box-and-whisker plot of mean ovarian tissue damage. Lesions estimated by cylinder or sphere volume. The upper line of the box represents the upper quartile, the thick line in the middle represents the median, and the lower line represents the lower quartile. Lesion volumes estimated by the two methods were statistically different between the two ovaries (*p = 0.021 and **p = 0.008).
Mentions: The measures of the lesioned areas calculated by cylindrical volume estimates showed a median of 1.12% in the right ovary and 1.65% in the left ovary. When estimation was calculated by spherical shape, the medians were 1.77% in the right ovary and 3.06% in the left ovary. There was a statistically significant difference in these two estimates of lesion area of the right ovaries (800 J) and left ovaries (1600 J) (p <0.05), as shown in Figure 4.

Bottom Line: No lesions could be found in the needle path.The ovaries were successfully cauterized without injuries in needle path and more energy resulted in significantly more thermal lesion.The safety and effectiveness of this technique, theoretically less invasive than current ovarian drilling methods, could be tested in anovulatory women with PCOS.

View Article: PubMed Central - HTML - PubMed

Affiliation: Programa de Pós-Graduação em Medicina: Ciências Médicas, Hospital de Clínicas de Porto Alegre, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil.

ABSTRACT

Background: To develop a minimally invasive ovarian cauterization technique under transvaginal ultrasound control and evaluate the safety and feasability of monopolar cauterization to cause ovarian injury using female cattle of reproductive age as an experimental model.

Method: Experimental study in a university research center was performed. Eleven female bovines of reproductive age were submitted to monopolar transvaginal ovarian cauterization. The right ovary (RO) was punctured at four sites and 40 W was applied for 5 s at each point, resulting in a total of 800 J (Joules) of thermal energy. In the left ovary (LO), the procedure was similar, with the same time and 80 W, resulting in a thermal energy of 1600 J. Macroscopic and microscopic lesions were assessed.

Results: Of 22 ovaries punctured, 20 were cauterized and exhibited macroscopic and typical microscopic lesions. No lesions could be found in the needle path. The measures of the areas of microscopic electrocautery lesions calculated estimating a cylindrical volume showed a median of 1.12% in the right ovary and 1.65% in the left ovary. When the estimate was calculated by spherical shape, the medians were 1.77% in the right ovary and 3.06% in the left ovary. There was a statistically significant difference in these two estimates (sphere, p = 0.008; cylinder, p = 0.021).

Conclusion: The experimental animal model described for transvaginal ultrasound-guided ovarian needle cauterization seems to be feasible. The ovaries were successfully cauterized without injuries in needle path and more energy resulted in significantly more thermal lesion. The safety and effectiveness of this technique, theoretically less invasive than current ovarian drilling methods, could be tested in anovulatory women with PCOS.

No MeSH data available.


Related in: MedlinePlus