Limits...
Developing transgenic Jatropha using the SbNHX1 gene from an extreme halophyte for cultivation in saline wasteland.

Joshi M, Jha A, Mishra A, Jha B - PLoS ONE (2013)

Bottom Line: Salt tolerance of the transgenic lines JL2, JL8 and JL19 was confirmed by leaf senescence assay, chlorophyll estimation, plant growth, ion content, electrolyte leakage and malondialdehyde (MDA) content analysis.Transgenic lines showed better salt tolerance than WT up to 200 mM NaCl.Apart from this, transgenic Jatropha can be cultivated with brackish water, opening up the possibility of sustainable cultivation of this biofuel plant in salty coastal areas.

View Article: PubMed Central - PubMed

Affiliation: Discipline of Marine Biotechnology and Ecology, CSIR-Central Salt and Marine Chemicals Research Institute, Bhavnagar, Gujarat, India. bjha@csmcri.org

ABSTRACT
Jatropha is an important second-generation biofuel plant. Salinity is a major factor adversely impacting the growth and yield of several plants including Jatropha. SbNHX1 is a vacuolar Na⁺/H⁺ antiporter gene that compartmentalises excess Na⁺ ions into the vacuole and maintains ion homeostasis. We have previously cloned and characterised the SbNHX1 gene from an extreme halophyte, Salicornia brachiata. Transgenic plants of Jatropha curcas with the SbNHX1 gene were developed using microprojectile bombardment mediated transformation. Integration of the transgene was confirmed by PCR and Rt-PCR and the copy number was determined by real time qPCR. The present study of engineering salt tolerance in Jatropha is the first report to date. Salt tolerance of the transgenic lines JL2, JL8 and JL19 was confirmed by leaf senescence assay, chlorophyll estimation, plant growth, ion content, electrolyte leakage and malondialdehyde (MDA) content analysis. Transgenic lines showed better salt tolerance than WT up to 200 mM NaCl. Imparting salt tolerance to Jatropha using the SbNHX1 gene may open up the possibility of cultivating it in marginal salty land, releasing arable land presently under Jatropha cultivation for agriculture purposes. Apart from this, transgenic Jatropha can be cultivated with brackish water, opening up the possibility of sustainable cultivation of this biofuel plant in salty coastal areas.

Show MeSH

Related in: MedlinePlus

Effect of 200 mM NaCl on in vitro growing transgenic lines JL2 and JL8, and WT after 21 days.
© Copyright Policy
Related In: Results  -  Collection


getmorefigures.php?uid=PMC3733712&req=5

pone-0071136-g006: Effect of 200 mM NaCl on in vitro growing transgenic lines JL2 and JL8, and WT after 21 days.

Mentions: The salt stress tolerance of T0 transgenic lines was studied by leaf disc senescence assay and chlorophyll estimation. Leaf discs from WT and T0 transgenic lines JL2, JL8 and JL19 were floated separately on 0, 50, 100, 150 and 200 mM NaCl for 8 days. Salinity-induced decreases in chlorophyll were lower in the SbNHX1-overexpressing lines compared to WT plants (Figure 4). The damage caused by salt stress was visualized by the degree of bleaching of leaf tissues. It was evident that the transgenic plants (JL2 and JL8) had a better ability to tolerate salinity stress. However, no significant difference in leaf senescence or chlorophyll content between WT and JL19 was observed. The chlorophyll content in the WT plants reduced significantly with increasing salt concentration while the transgenic lines (JL2 and JL8) retained higher amounts of chlorophyll than WT up to 200 mM NaCl (Figure 5a). Two months old transgenic lines and WT were transferred to SEM supplemented with 200 mM NaCl and kept for 21 days. Transgenic lines showed better growth, while WT showed bleaching in the leaves and growth was hindered (Figure 6).


Developing transgenic Jatropha using the SbNHX1 gene from an extreme halophyte for cultivation in saline wasteland.

Joshi M, Jha A, Mishra A, Jha B - PLoS ONE (2013)

Effect of 200 mM NaCl on in vitro growing transgenic lines JL2 and JL8, and WT after 21 days.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3733712&req=5

pone-0071136-g006: Effect of 200 mM NaCl on in vitro growing transgenic lines JL2 and JL8, and WT after 21 days.
Mentions: The salt stress tolerance of T0 transgenic lines was studied by leaf disc senescence assay and chlorophyll estimation. Leaf discs from WT and T0 transgenic lines JL2, JL8 and JL19 were floated separately on 0, 50, 100, 150 and 200 mM NaCl for 8 days. Salinity-induced decreases in chlorophyll were lower in the SbNHX1-overexpressing lines compared to WT plants (Figure 4). The damage caused by salt stress was visualized by the degree of bleaching of leaf tissues. It was evident that the transgenic plants (JL2 and JL8) had a better ability to tolerate salinity stress. However, no significant difference in leaf senescence or chlorophyll content between WT and JL19 was observed. The chlorophyll content in the WT plants reduced significantly with increasing salt concentration while the transgenic lines (JL2 and JL8) retained higher amounts of chlorophyll than WT up to 200 mM NaCl (Figure 5a). Two months old transgenic lines and WT were transferred to SEM supplemented with 200 mM NaCl and kept for 21 days. Transgenic lines showed better growth, while WT showed bleaching in the leaves and growth was hindered (Figure 6).

Bottom Line: Salt tolerance of the transgenic lines JL2, JL8 and JL19 was confirmed by leaf senescence assay, chlorophyll estimation, plant growth, ion content, electrolyte leakage and malondialdehyde (MDA) content analysis.Transgenic lines showed better salt tolerance than WT up to 200 mM NaCl.Apart from this, transgenic Jatropha can be cultivated with brackish water, opening up the possibility of sustainable cultivation of this biofuel plant in salty coastal areas.

View Article: PubMed Central - PubMed

Affiliation: Discipline of Marine Biotechnology and Ecology, CSIR-Central Salt and Marine Chemicals Research Institute, Bhavnagar, Gujarat, India. bjha@csmcri.org

ABSTRACT
Jatropha is an important second-generation biofuel plant. Salinity is a major factor adversely impacting the growth and yield of several plants including Jatropha. SbNHX1 is a vacuolar Na⁺/H⁺ antiporter gene that compartmentalises excess Na⁺ ions into the vacuole and maintains ion homeostasis. We have previously cloned and characterised the SbNHX1 gene from an extreme halophyte, Salicornia brachiata. Transgenic plants of Jatropha curcas with the SbNHX1 gene were developed using microprojectile bombardment mediated transformation. Integration of the transgene was confirmed by PCR and Rt-PCR and the copy number was determined by real time qPCR. The present study of engineering salt tolerance in Jatropha is the first report to date. Salt tolerance of the transgenic lines JL2, JL8 and JL19 was confirmed by leaf senescence assay, chlorophyll estimation, plant growth, ion content, electrolyte leakage and malondialdehyde (MDA) content analysis. Transgenic lines showed better salt tolerance than WT up to 200 mM NaCl. Imparting salt tolerance to Jatropha using the SbNHX1 gene may open up the possibility of cultivating it in marginal salty land, releasing arable land presently under Jatropha cultivation for agriculture purposes. Apart from this, transgenic Jatropha can be cultivated with brackish water, opening up the possibility of sustainable cultivation of this biofuel plant in salty coastal areas.

Show MeSH
Related in: MedlinePlus