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Proteome of human stem cells from periodontal ligament and dental pulp.

Eleuterio E, Trubiani O, Sulpizio M, Di Giuseppe F, Pierdomenico L, Marchisio M, Giancola R, Giammaria G, Miscia S, Caputi S, Di Ilio C, Angelucci S - PLoS ONE (2013)

Bottom Line: Human adult stem cells (SCs) including bone marrow stem cells (BMSCs), dental pulp stem cells (DPSCs) and periodontal ligament stem cells (PDLSCs) have been characterized for their high proliferative potential, expression of characteristic SC-associated markers and for the plasticity to differentiate in different lineage in vitro.To enrich the knowledge of oral SCs proteome we performed an analysis in narrow range pH 4-7 and 6-9, and we found that DPSCs vs PDLSCs express differentially regulated proteins that are potentially related to growth, regulation and genesis of neuronal cells, suggesting that SCs derived from oral tissue source populations may possess the potential ability of neuronal differentiation which is very consistent with their neural crest origin.This study identifies some differentially expressed proteins by using comparative analysis between DPSCs and PDLSCs and BMSCs and suggests that stem cells from oral tissue could have a different cell lineage potency compared to BMSCs.

View Article: PubMed Central - PubMed

Affiliation: Department of Experimental and Clinical Sciences, G. d'Annunzio University, Chieti-Pescara, Italy.

ABSTRACT

Background: Many adult tissues contain a population of stem cells with the ability to regenerate structures similar to the microenvironments from which they are derived in vivo and represent a promising therapy for the regeneration of complex tissues in the clinical disorder. Human adult stem cells (SCs) including bone marrow stem cells (BMSCs), dental pulp stem cells (DPSCs) and periodontal ligament stem cells (PDLSCs) have been characterized for their high proliferative potential, expression of characteristic SC-associated markers and for the plasticity to differentiate in different lineage in vitro.

Methodology/principal findings: The aim of this study is to define the molecular features of stem cells from oral tissue by comparing the proteomic profiles obtained with 2-DE followed by MALDI-TOF/TOF of ex-vivo cultured human PDLSCs, DPSCs and BMSCs. Our results showed qualitative similarities in the proteome profiles among the SCs examined including some significant quantitative differences. To enrich the knowledge of oral SCs proteome we performed an analysis in narrow range pH 4-7 and 6-9, and we found that DPSCs vs PDLSCs express differentially regulated proteins that are potentially related to growth, regulation and genesis of neuronal cells, suggesting that SCs derived from oral tissue source populations may possess the potential ability of neuronal differentiation which is very consistent with their neural crest origin.

Conclusion/significance: This study identifies some differentially expressed proteins by using comparative analysis between DPSCs and PDLSCs and BMSCs and suggests that stem cells from oral tissue could have a different cell lineage potency compared to BMSCs.

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Photomicrographs of primary cultures of BMSCs (A) DPSCs (B) and PDLSCs (C) at passage 2 analyzed at morphological and cytofluorimetric levels.At Scanning (section A, B, and C) and Light microscopy (a1,b1,c1) the culture displays the comparable morphological features consisting of adherent cells having a morphological homogeneous fibroblast-like appearance with a stellate shape and long cytoplasmic processes and numerous filopodia. Nuclei contain one or more nucleoli (Nc). At 28 days of culture in the osteogenic medium BMSCs, DPSCs and PDLSCs (a2,b2,c2) differentiate versus osteoblasts as evidenced by calcium deposition with Alizarin Red staining. Original magnification: 1.000× (scanning microscopy), 10× (light microscopy). The histograms show the cytofluorimetric analysis of the cell culture BMSCs, DPSCs and PDLSCs, surface and intracellular antigens expression profile: CD13, CD14, CD29, CD34, CD44, CD45, CD73, CD90, CD105, CD117 CD133, CD144, CD146, CD166, CD271, OCT3/4, Sox2 and SSEA4. Blue histograms represent cells stained with the expression markers; red histograms show the respective IgG isotype control. These data are representative of four independent biological samples.
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pone-0071101-g001: Photomicrographs of primary cultures of BMSCs (A) DPSCs (B) and PDLSCs (C) at passage 2 analyzed at morphological and cytofluorimetric levels.At Scanning (section A, B, and C) and Light microscopy (a1,b1,c1) the culture displays the comparable morphological features consisting of adherent cells having a morphological homogeneous fibroblast-like appearance with a stellate shape and long cytoplasmic processes and numerous filopodia. Nuclei contain one or more nucleoli (Nc). At 28 days of culture in the osteogenic medium BMSCs, DPSCs and PDLSCs (a2,b2,c2) differentiate versus osteoblasts as evidenced by calcium deposition with Alizarin Red staining. Original magnification: 1.000× (scanning microscopy), 10× (light microscopy). The histograms show the cytofluorimetric analysis of the cell culture BMSCs, DPSCs and PDLSCs, surface and intracellular antigens expression profile: CD13, CD14, CD29, CD34, CD44, CD45, CD73, CD90, CD105, CD117 CD133, CD144, CD146, CD166, CD271, OCT3/4, Sox2 and SSEA4. Blue histograms represent cells stained with the expression markers; red histograms show the respective IgG isotype control. These data are representative of four independent biological samples.

Mentions: In this study we compared BMSCs, DPSCs and PDLSCs at passage 2, when the highest proliferative rate occurs. Under light microscopy, the primary cultures of SCs consisting of colonies of adherent cells showed a morphologically homogeneous fibroblast-like shape. As usual, the cells adhered to each other forming colonies, the nuclei were round or oval-shaped with abundant euchromatin, indicative of an active gene transcription (Fig. 1, insert a1, b1, c1).


Proteome of human stem cells from periodontal ligament and dental pulp.

Eleuterio E, Trubiani O, Sulpizio M, Di Giuseppe F, Pierdomenico L, Marchisio M, Giancola R, Giammaria G, Miscia S, Caputi S, Di Ilio C, Angelucci S - PLoS ONE (2013)

Photomicrographs of primary cultures of BMSCs (A) DPSCs (B) and PDLSCs (C) at passage 2 analyzed at morphological and cytofluorimetric levels.At Scanning (section A, B, and C) and Light microscopy (a1,b1,c1) the culture displays the comparable morphological features consisting of adherent cells having a morphological homogeneous fibroblast-like appearance with a stellate shape and long cytoplasmic processes and numerous filopodia. Nuclei contain one or more nucleoli (Nc). At 28 days of culture in the osteogenic medium BMSCs, DPSCs and PDLSCs (a2,b2,c2) differentiate versus osteoblasts as evidenced by calcium deposition with Alizarin Red staining. Original magnification: 1.000× (scanning microscopy), 10× (light microscopy). The histograms show the cytofluorimetric analysis of the cell culture BMSCs, DPSCs and PDLSCs, surface and intracellular antigens expression profile: CD13, CD14, CD29, CD34, CD44, CD45, CD73, CD90, CD105, CD117 CD133, CD144, CD146, CD166, CD271, OCT3/4, Sox2 and SSEA4. Blue histograms represent cells stained with the expression markers; red histograms show the respective IgG isotype control. These data are representative of four independent biological samples.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3733711&req=5

pone-0071101-g001: Photomicrographs of primary cultures of BMSCs (A) DPSCs (B) and PDLSCs (C) at passage 2 analyzed at morphological and cytofluorimetric levels.At Scanning (section A, B, and C) and Light microscopy (a1,b1,c1) the culture displays the comparable morphological features consisting of adherent cells having a morphological homogeneous fibroblast-like appearance with a stellate shape and long cytoplasmic processes and numerous filopodia. Nuclei contain one or more nucleoli (Nc). At 28 days of culture in the osteogenic medium BMSCs, DPSCs and PDLSCs (a2,b2,c2) differentiate versus osteoblasts as evidenced by calcium deposition with Alizarin Red staining. Original magnification: 1.000× (scanning microscopy), 10× (light microscopy). The histograms show the cytofluorimetric analysis of the cell culture BMSCs, DPSCs and PDLSCs, surface and intracellular antigens expression profile: CD13, CD14, CD29, CD34, CD44, CD45, CD73, CD90, CD105, CD117 CD133, CD144, CD146, CD166, CD271, OCT3/4, Sox2 and SSEA4. Blue histograms represent cells stained with the expression markers; red histograms show the respective IgG isotype control. These data are representative of four independent biological samples.
Mentions: In this study we compared BMSCs, DPSCs and PDLSCs at passage 2, when the highest proliferative rate occurs. Under light microscopy, the primary cultures of SCs consisting of colonies of adherent cells showed a morphologically homogeneous fibroblast-like shape. As usual, the cells adhered to each other forming colonies, the nuclei were round or oval-shaped with abundant euchromatin, indicative of an active gene transcription (Fig. 1, insert a1, b1, c1).

Bottom Line: Human adult stem cells (SCs) including bone marrow stem cells (BMSCs), dental pulp stem cells (DPSCs) and periodontal ligament stem cells (PDLSCs) have been characterized for their high proliferative potential, expression of characteristic SC-associated markers and for the plasticity to differentiate in different lineage in vitro.To enrich the knowledge of oral SCs proteome we performed an analysis in narrow range pH 4-7 and 6-9, and we found that DPSCs vs PDLSCs express differentially regulated proteins that are potentially related to growth, regulation and genesis of neuronal cells, suggesting that SCs derived from oral tissue source populations may possess the potential ability of neuronal differentiation which is very consistent with their neural crest origin.This study identifies some differentially expressed proteins by using comparative analysis between DPSCs and PDLSCs and BMSCs and suggests that stem cells from oral tissue could have a different cell lineage potency compared to BMSCs.

View Article: PubMed Central - PubMed

Affiliation: Department of Experimental and Clinical Sciences, G. d'Annunzio University, Chieti-Pescara, Italy.

ABSTRACT

Background: Many adult tissues contain a population of stem cells with the ability to regenerate structures similar to the microenvironments from which they are derived in vivo and represent a promising therapy for the regeneration of complex tissues in the clinical disorder. Human adult stem cells (SCs) including bone marrow stem cells (BMSCs), dental pulp stem cells (DPSCs) and periodontal ligament stem cells (PDLSCs) have been characterized for their high proliferative potential, expression of characteristic SC-associated markers and for the plasticity to differentiate in different lineage in vitro.

Methodology/principal findings: The aim of this study is to define the molecular features of stem cells from oral tissue by comparing the proteomic profiles obtained with 2-DE followed by MALDI-TOF/TOF of ex-vivo cultured human PDLSCs, DPSCs and BMSCs. Our results showed qualitative similarities in the proteome profiles among the SCs examined including some significant quantitative differences. To enrich the knowledge of oral SCs proteome we performed an analysis in narrow range pH 4-7 and 6-9, and we found that DPSCs vs PDLSCs express differentially regulated proteins that are potentially related to growth, regulation and genesis of neuronal cells, suggesting that SCs derived from oral tissue source populations may possess the potential ability of neuronal differentiation which is very consistent with their neural crest origin.

Conclusion/significance: This study identifies some differentially expressed proteins by using comparative analysis between DPSCs and PDLSCs and BMSCs and suggests that stem cells from oral tissue could have a different cell lineage potency compared to BMSCs.

Show MeSH
Related in: MedlinePlus