Limits...
Gender-mediated differences in vertical transmission of a nucleopolyhedrovirus.

Virto C, Zárate CA, López-Ferber M, Murillo R, Caballero P, Williams T - PLoS ONE (2013)

Bottom Line: Experimental adults and their offspring were analyzed by qPCR to determine the prevalence of infection.Both males and females were able to transmit the infection to the next generation, although female-mediated transmission resulted in a higher prevalence of infected offspring.Both male and female offspring were infected by their parents in similar proportions.

View Article: PubMed Central - PubMed

Affiliation: Instituto de Agrobiotecnología, CSIC-Gobierno de Navarra, Mutilva Baja, Navarra, Spain.

ABSTRACT
With the development of sensitive molecular techniques for detection of low levels of asymptomatic pathogens, it becoming clear that vertical transmission is a common feature of some insect pathogenic viruses, and likely to be essential to virus survival when opportunities for horizontal transmission are unfavorable. Vertical transmission of Spodoptera exigua multiple nucleopolyhedrovirus (SeMNPV) is common in natural populations of S. exigua. To assess whether gender affected transgenerational virus transmission, four mating group treatments were performed using healthy and sublethally infected insects: i) healthy males (H♂) × healthy females (H♀); ii) infected males (I♂) × healthy females (H♀); iii) healthy males (H♂) × infected females (I♀) and iv) infected males (I♂) × infected females (I♀). Experimental adults and their offspring were analyzed by qPCR to determine the prevalence of infection. Both males and females were able to transmit the infection to the next generation, although female-mediated transmission resulted in a higher prevalence of infected offspring. Male-mediated venereal transmission was half as efficient as maternally-mediated transmission. Egg surface decontamination studies indicated that the main route of transmission is likely transovarial rather than transovum. Both male and female offspring were infected by their parents in similar proportions. Incorporating vertically-transmitted genotypes into virus-based insecticides could provide moderate levels of transgenerational pest control, thereby extending the periods between bioinsecticide applications.

Show MeSH

Related in: MedlinePlus

Prevalence of infection in parental adults in each mating group.H♂: Healthy male, H♀: Healthy female, I♂: Infected male, I♀: Infected female. Each mating group comprised 20 male and female S. exigua moths.
© Copyright Policy
Related In: Results  -  Collection


getmorefigures.php?uid=PMC3733637&req=5

pone-0070932-g001: Prevalence of infection in parental adults in each mating group.H♂: Healthy male, H♀: Healthy female, I♂: Infected male, I♀: Infected female. Each mating group comprised 20 male and female S. exigua moths.

Mentions: Overall, 57.6±4.4% of the larvae that consumed VT-SeAl1 OBs succumbed to virus infection, whereas no mortality was registered in mock-infected control larvae. The prevalence of qPCR positive reactions in the insects that survived, following consumption of viral OBs in the larval stage, was clearly higher than that of control insects (Figure 1). Viral load in parental (F0) adults averaged 1.514±0.287×10−3 pg viral DNA/µg total DNA per insect (N = 72 positive samples) that represents 10.34±1.96 genome copies per reaction.


Gender-mediated differences in vertical transmission of a nucleopolyhedrovirus.

Virto C, Zárate CA, López-Ferber M, Murillo R, Caballero P, Williams T - PLoS ONE (2013)

Prevalence of infection in parental adults in each mating group.H♂: Healthy male, H♀: Healthy female, I♂: Infected male, I♀: Infected female. Each mating group comprised 20 male and female S. exigua moths.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3733637&req=5

pone-0070932-g001: Prevalence of infection in parental adults in each mating group.H♂: Healthy male, H♀: Healthy female, I♂: Infected male, I♀: Infected female. Each mating group comprised 20 male and female S. exigua moths.
Mentions: Overall, 57.6±4.4% of the larvae that consumed VT-SeAl1 OBs succumbed to virus infection, whereas no mortality was registered in mock-infected control larvae. The prevalence of qPCR positive reactions in the insects that survived, following consumption of viral OBs in the larval stage, was clearly higher than that of control insects (Figure 1). Viral load in parental (F0) adults averaged 1.514±0.287×10−3 pg viral DNA/µg total DNA per insect (N = 72 positive samples) that represents 10.34±1.96 genome copies per reaction.

Bottom Line: Experimental adults and their offspring were analyzed by qPCR to determine the prevalence of infection.Both males and females were able to transmit the infection to the next generation, although female-mediated transmission resulted in a higher prevalence of infected offspring.Both male and female offspring were infected by their parents in similar proportions.

View Article: PubMed Central - PubMed

Affiliation: Instituto de Agrobiotecnología, CSIC-Gobierno de Navarra, Mutilva Baja, Navarra, Spain.

ABSTRACT
With the development of sensitive molecular techniques for detection of low levels of asymptomatic pathogens, it becoming clear that vertical transmission is a common feature of some insect pathogenic viruses, and likely to be essential to virus survival when opportunities for horizontal transmission are unfavorable. Vertical transmission of Spodoptera exigua multiple nucleopolyhedrovirus (SeMNPV) is common in natural populations of S. exigua. To assess whether gender affected transgenerational virus transmission, four mating group treatments were performed using healthy and sublethally infected insects: i) healthy males (H♂) × healthy females (H♀); ii) infected males (I♂) × healthy females (H♀); iii) healthy males (H♂) × infected females (I♀) and iv) infected males (I♂) × infected females (I♀). Experimental adults and their offspring were analyzed by qPCR to determine the prevalence of infection. Both males and females were able to transmit the infection to the next generation, although female-mediated transmission resulted in a higher prevalence of infected offspring. Male-mediated venereal transmission was half as efficient as maternally-mediated transmission. Egg surface decontamination studies indicated that the main route of transmission is likely transovarial rather than transovum. Both male and female offspring were infected by their parents in similar proportions. Incorporating vertically-transmitted genotypes into virus-based insecticides could provide moderate levels of transgenerational pest control, thereby extending the periods between bioinsecticide applications.

Show MeSH
Related in: MedlinePlus