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Effect of various concentrations of tetracycline hydrochloride demineralization on root dentin surface: A scanning electron microscopic study.

Penmatsa T, Varma S - J Pharm Bioallied Sci (2013)

Bottom Line: Mean values were compared by either one-way ANOVA with Tukey-Honestly significant difference procedure or Kruskal - Wallis one way ANOVA with Mann-Whitney u-test.In the present study using 100 mg/ml tetracycline seems to be more effective on both diseased and non-diseased dentin surfaces.The results of this study confirm that pure TTC-HCl conditioning produced comparable surface characteristics on dentin of both diseased and non-diseased roots with 100 mg/ml concentration for 3 min.

View Article: PubMed Central - PubMed

Affiliation: Department of Periodontics, Gitam Dental College and Hospital, Vishakhapatnam, Andhra Pradesh, India.

ABSTRACT

Introduction: Within the last 20 years root demineralization has attracted attention as a periodontal regenerative technique. Topical tetracycline application has been widely reported for use as a conditioner, to decontaminate the root surface and to promote periodontal regeneration. The purpose of this scanning electron microscopic (SEM) study is to evaluate the surface characteristics of demineralized diseased and non-diseased dentine root surfaces using different concentrations of tetracycline hydrochloride (TTC-HCl).

Material and methods: This SEM study was carried out in 20 dentin samples obtained from non-diseased human premolars and 20 dentin samples obtained from diseased human premolars. Pure TTC-HCl was applied to the dentin surface at 50 mg/ml, 100 mg/ml and 150 mg/ml concentrations for 3 min in experimental groups while distilled (0 mg/ml) water was applied to the control groups.

Statistical analysis: Mean and standard deviation were estimated from the sample for each study group. Mean values were compared by either one-way ANOVA with Tukey-Honestly significant difference procedure or Kruskal - Wallis one way ANOVA with Mann-Whitney u-test. Further Student's independent t-test/Mann-Whitney u-test was used appropriately to compare the mean values between two independent groups.

Results: In the present study using 100 mg/ml tetracycline seems to be more effective on both diseased and non-diseased dentin surfaces.

Conclusions: The results of this study confirm that pure TTC-HCl conditioning produced comparable surface characteristics on dentin of both diseased and non-diseased roots with 100 mg/ml concentration for 3 min.

No MeSH data available.


Related in: MedlinePlus

Scanning electron microscopic photograph of diseased dentin surface treated with distilled water
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Figure 1: Scanning electron microscopic photograph of diseased dentin surface treated with distilled water

Mentions: Mean number of exposed dentinal tubules using 100 mg/ml was significantly higher than 0 mg/ml, 50 mg/ml, and 150 mg/ml [1–4]. Mean diameter of the dentinal tubules using 100 mg/ml is significantly higher than that of using 0 mg/ml, 50 mg/ml, 150 mg/ml [1–4]. Mean smear layer score in group F (3.6 ± 0.5), Group G (4.0 ± 0.0) and group H (4.0 ± 0.0) are significantly higher than Group E (0.0 ± 0.0) (P < 0.05). There was no significant difference in mean smear layer score between other contrasts (P > 0.05).


Effect of various concentrations of tetracycline hydrochloride demineralization on root dentin surface: A scanning electron microscopic study.

Penmatsa T, Varma S - J Pharm Bioallied Sci (2013)

Scanning electron microscopic photograph of diseased dentin surface treated with distilled water
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3722705&req=5

Figure 1: Scanning electron microscopic photograph of diseased dentin surface treated with distilled water
Mentions: Mean number of exposed dentinal tubules using 100 mg/ml was significantly higher than 0 mg/ml, 50 mg/ml, and 150 mg/ml [1–4]. Mean diameter of the dentinal tubules using 100 mg/ml is significantly higher than that of using 0 mg/ml, 50 mg/ml, 150 mg/ml [1–4]. Mean smear layer score in group F (3.6 ± 0.5), Group G (4.0 ± 0.0) and group H (4.0 ± 0.0) are significantly higher than Group E (0.0 ± 0.0) (P < 0.05). There was no significant difference in mean smear layer score between other contrasts (P > 0.05).

Bottom Line: Mean values were compared by either one-way ANOVA with Tukey-Honestly significant difference procedure or Kruskal - Wallis one way ANOVA with Mann-Whitney u-test.In the present study using 100 mg/ml tetracycline seems to be more effective on both diseased and non-diseased dentin surfaces.The results of this study confirm that pure TTC-HCl conditioning produced comparable surface characteristics on dentin of both diseased and non-diseased roots with 100 mg/ml concentration for 3 min.

View Article: PubMed Central - PubMed

Affiliation: Department of Periodontics, Gitam Dental College and Hospital, Vishakhapatnam, Andhra Pradesh, India.

ABSTRACT

Introduction: Within the last 20 years root demineralization has attracted attention as a periodontal regenerative technique. Topical tetracycline application has been widely reported for use as a conditioner, to decontaminate the root surface and to promote periodontal regeneration. The purpose of this scanning electron microscopic (SEM) study is to evaluate the surface characteristics of demineralized diseased and non-diseased dentine root surfaces using different concentrations of tetracycline hydrochloride (TTC-HCl).

Material and methods: This SEM study was carried out in 20 dentin samples obtained from non-diseased human premolars and 20 dentin samples obtained from diseased human premolars. Pure TTC-HCl was applied to the dentin surface at 50 mg/ml, 100 mg/ml and 150 mg/ml concentrations for 3 min in experimental groups while distilled (0 mg/ml) water was applied to the control groups.

Statistical analysis: Mean and standard deviation were estimated from the sample for each study group. Mean values were compared by either one-way ANOVA with Tukey-Honestly significant difference procedure or Kruskal - Wallis one way ANOVA with Mann-Whitney u-test. Further Student's independent t-test/Mann-Whitney u-test was used appropriately to compare the mean values between two independent groups.

Results: In the present study using 100 mg/ml tetracycline seems to be more effective on both diseased and non-diseased dentin surfaces.

Conclusions: The results of this study confirm that pure TTC-HCl conditioning produced comparable surface characteristics on dentin of both diseased and non-diseased roots with 100 mg/ml concentration for 3 min.

No MeSH data available.


Related in: MedlinePlus