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Distribution of CC-chemokine receptor-5-∆32 allele among the tribal and caste population of Vidarbha region of Maharashtra state.

Chavhan AB, Pawar SS, Jadhao RG, Patil KG - Indian J Hum Genet (2013)

Bottom Line: Considering this assumption, we analyzed the frequency of the CC-chemokine receptor-5 (CCR5)-∆32 allele of the CCR5 chemokine receptor, which is considered a Caucasian marker, in Bhil tribal and Brahmin caste sample sets from the population. 108 blood samples were collected from 6 tribe's populations and a caste population from the district of Vidarbha region.The marginal presence of the allele seen in the studied tribal population could be due to gene flow from the people of European descent.However, lack of the homozygous CCR5-Δ32 mutation and the low prevalence of heterozygous CCR5-Δ32 mutations suggest that the Indians are highly susceptible to HIV/AIDS, and this correlates with the highest number of HIV/AIDS infected individuals in India.

View Article: PubMed Central - PubMed

Affiliation: Department of Zoology, Shri Shivaji Science College, Amravati, India ; Department of Zoology, Institute of Science, Nagpur, India.

ABSTRACT

Background: Genetic relationships among the ethnic groups are not uniform across the geographical region. Considering this assumption, we analyzed the frequency of the CC-chemokine receptor-5 (CCR5)-∆32 allele of the CCR5 chemokine receptor, which is considered a Caucasian marker, in Bhil tribal and Brahmin caste sample sets from the population.

Materials and methods: 108 blood samples were collected from 6 tribe's populations and a caste population from the district of Vidarbha region.

Results and discussion: The presence of low frequencies of CCR5-Δ32 in an individual of Bhil tribe (0.034, χ(2) value 0.017) in the present study implies that these communities may have a better resistance toward human immunodeficiency virus (HIV)/acquired immunodeficiency syndrome (AIDS) than the other studied tribe sample, as non-show such mutation.

Conclusion: The marginal presence of the allele seen in the studied tribal population could be due to gene flow from the people of European descent. However, lack of the homozygous CCR5-Δ32 mutation and the low prevalence of heterozygous CCR5-Δ32 mutations suggest that the Indians are highly susceptible to HIV/AIDS, and this correlates with the highest number of HIV/AIDS infected individuals in India.

No MeSH data available.


Related in: MedlinePlus

CC-chemokine receptor-5 genotyping among the tribes Lane 1, 2, 3, 5, 6 and 7 represent the PCR product from samples with homozygous wild type genotypes (fragments of 332 bp wt/wt). Lane 4 represents the Δ-32 genotype (with the presence of both fragment of 332 and 403 wt/Δ32)
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Figure 1: CC-chemokine receptor-5 genotyping among the tribes Lane 1, 2, 3, 5, 6 and 7 represent the PCR product from samples with homozygous wild type genotypes (fragments of 332 bp wt/wt). Lane 4 represents the Δ-32 genotype (with the presence of both fragment of 332 and 403 wt/Δ32)

Mentions: The genotypes were visualized by running digested product on 2% agarose gel at 100 V for about 2 h and the results were recorded in gel documentation system. The EcoRI Restriction enzymes digest the amplified polymerization Chain Reaction (PCR) product of 735 base pairs (bps). The amplified product was digested with 10 U of EcoRI at 37°C for 2 h. After digestion, the products were analyzed on a 2% agarose gel and bands were visualized on a Ultraviolet (UV)-transilluminator. PCR amplified a 735 bp region of genomic DNA that spanned a 32-bp deletion differentiating the CCR5-_32 allele from its wild-type counterparts at the CCR5 locus. After restriction digestion with EcoRI, with wild gene yielded band at 332 bp and for mutated gene, the bands were at 332 and 403 Figure 1.


Distribution of CC-chemokine receptor-5-∆32 allele among the tribal and caste population of Vidarbha region of Maharashtra state.

Chavhan AB, Pawar SS, Jadhao RG, Patil KG - Indian J Hum Genet (2013)

CC-chemokine receptor-5 genotyping among the tribes Lane 1, 2, 3, 5, 6 and 7 represent the PCR product from samples with homozygous wild type genotypes (fragments of 332 bp wt/wt). Lane 4 represents the Δ-32 genotype (with the presence of both fragment of 332 and 403 wt/Δ32)
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3722632&req=5

Figure 1: CC-chemokine receptor-5 genotyping among the tribes Lane 1, 2, 3, 5, 6 and 7 represent the PCR product from samples with homozygous wild type genotypes (fragments of 332 bp wt/wt). Lane 4 represents the Δ-32 genotype (with the presence of both fragment of 332 and 403 wt/Δ32)
Mentions: The genotypes were visualized by running digested product on 2% agarose gel at 100 V for about 2 h and the results were recorded in gel documentation system. The EcoRI Restriction enzymes digest the amplified polymerization Chain Reaction (PCR) product of 735 base pairs (bps). The amplified product was digested with 10 U of EcoRI at 37°C for 2 h. After digestion, the products were analyzed on a 2% agarose gel and bands were visualized on a Ultraviolet (UV)-transilluminator. PCR amplified a 735 bp region of genomic DNA that spanned a 32-bp deletion differentiating the CCR5-_32 allele from its wild-type counterparts at the CCR5 locus. After restriction digestion with EcoRI, with wild gene yielded band at 332 bp and for mutated gene, the bands were at 332 and 403 Figure 1.

Bottom Line: Considering this assumption, we analyzed the frequency of the CC-chemokine receptor-5 (CCR5)-∆32 allele of the CCR5 chemokine receptor, which is considered a Caucasian marker, in Bhil tribal and Brahmin caste sample sets from the population. 108 blood samples were collected from 6 tribe's populations and a caste population from the district of Vidarbha region.The marginal presence of the allele seen in the studied tribal population could be due to gene flow from the people of European descent.However, lack of the homozygous CCR5-Δ32 mutation and the low prevalence of heterozygous CCR5-Δ32 mutations suggest that the Indians are highly susceptible to HIV/AIDS, and this correlates with the highest number of HIV/AIDS infected individuals in India.

View Article: PubMed Central - PubMed

Affiliation: Department of Zoology, Shri Shivaji Science College, Amravati, India ; Department of Zoology, Institute of Science, Nagpur, India.

ABSTRACT

Background: Genetic relationships among the ethnic groups are not uniform across the geographical region. Considering this assumption, we analyzed the frequency of the CC-chemokine receptor-5 (CCR5)-∆32 allele of the CCR5 chemokine receptor, which is considered a Caucasian marker, in Bhil tribal and Brahmin caste sample sets from the population.

Materials and methods: 108 blood samples were collected from 6 tribe's populations and a caste population from the district of Vidarbha region.

Results and discussion: The presence of low frequencies of CCR5-Δ32 in an individual of Bhil tribe (0.034, χ(2) value 0.017) in the present study implies that these communities may have a better resistance toward human immunodeficiency virus (HIV)/acquired immunodeficiency syndrome (AIDS) than the other studied tribe sample, as non-show such mutation.

Conclusion: The marginal presence of the allele seen in the studied tribal population could be due to gene flow from the people of European descent. However, lack of the homozygous CCR5-Δ32 mutation and the low prevalence of heterozygous CCR5-Δ32 mutations suggest that the Indians are highly susceptible to HIV/AIDS, and this correlates with the highest number of HIV/AIDS infected individuals in India.

No MeSH data available.


Related in: MedlinePlus