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Investigation of cytocrom c oxidase gene subunits expression on the Multiple sclerosis.

Safavizadeh N, Rahmani SA, Zaefizadeh M - Indian J Hum Genet (2013)

Bottom Line: Numerous studies have reported mitochondrial defects including a reduction in cytochrome c oxidase (COX) complex function related to the reduction of mitochondrial genes expression in the cortex tissue of patients with MS have been reported.The resultshowed COX5B gene expression reduced significant in MS patients compared to normal subjects (P < -0.05) whereas, there was no significant difference in the COX2 gene expression between normal subjects and patients.Thus, it can be claimed that down-regulation of mitochondrial electron transport chain genes supported the hypothesis that hypoxia-like tissue injury in MS may be due to mitochondrial genes, different expression impairment.

View Article: PubMed Central - PubMed

Affiliation: Department of Biology, Ahar Branch, IAU university, Ahar, Iran.

ABSTRACT

Introduction: Multiple sclerosis (MS) is an autoimmune inflamatory disease, which affects the (Central Nervous System) and leads to the destruction of myelin and atrophy of the axons. Genetic factors, in addition to environmental ones, seem to play a role in MS. Numerous studies have reported mitochondrial defects including a reduction in cytochrome c oxidase (COX) complex function related to the reduction of mitochondrial genes expression in the cortex tissue of patients with MS have been reported.

Materials and methods: This study aimed to assess COX5B and COX2 genes expression in MS patients and compare it with normal subjects. We determine expression levels of genes COX5B and COX2, and also gene reference ß-actine using real-time polymerase chain reaction (RT-PCR) method. Data were obtained and obtained and standardized with the gene reference and were analyzed using independent sample t-test with SPSS and Excel programs.

Result and discussion: The resultshowed COX5B gene expression reduced significant in MS patients compared to normal subjects (P < -0.05) whereas, there was no significant difference in the COX2 gene expression between normal subjects and patients. Thus, it can be claimed that down-regulation of mitochondrial electron transport chain genes supported the hypothesis that hypoxia-like tissue injury in MS may be due to mitochondrial genes, different expression impairment.

No MeSH data available.


Related in: MedlinePlus

Ct of the target gene in subjects’ cytochrome c oxidase 5B
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Figure 2: Ct of the target gene in subjects’ cytochrome c oxidase 5B

Mentions: Quantitative measurement of (COX2, COX5B) genes expression was investigated in 30 MS patients (12 males and 18 females). Five different standard concentrations were used to draw a standard curve [Figure 1]. Quantitative measurement of genes expression achieved in the PCR of the cycles was carried out in progressive phase. RT-PCR Software can determine Threshold Cycle automatically [Figure 2]. Furthermore, due to temperature changes in melting curve analysis. There is only one peak associated with the COX5B and COX2 genes, which is a symptom of lack of cases such as non–specific products and primer dimer [Figure 3]. After the PCR, as a template standard DNA of successive dilutions, standard curve was plotted for the COX5B and COX2 genes [Figure 4]. Standard curve of COX5B gene obtained determination of index (R2) = 0.98 and also standard curve COX2 gene obtained detremination of index (R2) = 0.95.


Investigation of cytocrom c oxidase gene subunits expression on the Multiple sclerosis.

Safavizadeh N, Rahmani SA, Zaefizadeh M - Indian J Hum Genet (2013)

Ct of the target gene in subjects’ cytochrome c oxidase 5B
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3722625&req=5

Figure 2: Ct of the target gene in subjects’ cytochrome c oxidase 5B
Mentions: Quantitative measurement of (COX2, COX5B) genes expression was investigated in 30 MS patients (12 males and 18 females). Five different standard concentrations were used to draw a standard curve [Figure 1]. Quantitative measurement of genes expression achieved in the PCR of the cycles was carried out in progressive phase. RT-PCR Software can determine Threshold Cycle automatically [Figure 2]. Furthermore, due to temperature changes in melting curve analysis. There is only one peak associated with the COX5B and COX2 genes, which is a symptom of lack of cases such as non–specific products and primer dimer [Figure 3]. After the PCR, as a template standard DNA of successive dilutions, standard curve was plotted for the COX5B and COX2 genes [Figure 4]. Standard curve of COX5B gene obtained determination of index (R2) = 0.98 and also standard curve COX2 gene obtained detremination of index (R2) = 0.95.

Bottom Line: Numerous studies have reported mitochondrial defects including a reduction in cytochrome c oxidase (COX) complex function related to the reduction of mitochondrial genes expression in the cortex tissue of patients with MS have been reported.The resultshowed COX5B gene expression reduced significant in MS patients compared to normal subjects (P < -0.05) whereas, there was no significant difference in the COX2 gene expression between normal subjects and patients.Thus, it can be claimed that down-regulation of mitochondrial electron transport chain genes supported the hypothesis that hypoxia-like tissue injury in MS may be due to mitochondrial genes, different expression impairment.

View Article: PubMed Central - PubMed

Affiliation: Department of Biology, Ahar Branch, IAU university, Ahar, Iran.

ABSTRACT

Introduction: Multiple sclerosis (MS) is an autoimmune inflamatory disease, which affects the (Central Nervous System) and leads to the destruction of myelin and atrophy of the axons. Genetic factors, in addition to environmental ones, seem to play a role in MS. Numerous studies have reported mitochondrial defects including a reduction in cytochrome c oxidase (COX) complex function related to the reduction of mitochondrial genes expression in the cortex tissue of patients with MS have been reported.

Materials and methods: This study aimed to assess COX5B and COX2 genes expression in MS patients and compare it with normal subjects. We determine expression levels of genes COX5B and COX2, and also gene reference ß-actine using real-time polymerase chain reaction (RT-PCR) method. Data were obtained and obtained and standardized with the gene reference and were analyzed using independent sample t-test with SPSS and Excel programs.

Result and discussion: The resultshowed COX5B gene expression reduced significant in MS patients compared to normal subjects (P < -0.05) whereas, there was no significant difference in the COX2 gene expression between normal subjects and patients. Thus, it can be claimed that down-regulation of mitochondrial electron transport chain genes supported the hypothesis that hypoxia-like tissue injury in MS may be due to mitochondrial genes, different expression impairment.

No MeSH data available.


Related in: MedlinePlus