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Experimental cross-species infection of common marmosets by titi monkey adenovirus.

Yu G, Yagi S, Carrion R, Chen EC, Liu M, Brasky KM, Lanford RE, Kelly KR, Bales KL, Schnurr DP, Canfield DR, Patterson JL, Chiu CY - PLoS ONE (2013)

Bottom Line: Here we present experimental evidence of cross-species TMAdV infection in common marmosets (Callithrix jacchus).Nasal inoculation of a cell cultured-adapted TMAdV strain into three marmosets produced an acute, mild respiratory illness characterized by low-grade fever, reduced activity, anorexia, and sneezing.An increase in virus-specific neutralization antibody titers accompanied the development of clinical signs.

View Article: PubMed Central - PubMed

Affiliation: Department of Laboratory Medicine, University of California San Francisco, San Francisco, California, United States of America.

ABSTRACT
Adenoviruses are DNA viruses that infect a number of vertebrate hosts and are associated with both sporadic and epidemic disease in humans. We previously identified a novel adenovirus, titi monkey adenovirus (TMAdV), as the cause of a fulminant pneumonia outbreak in a colony of titi monkeys (Callicebus cupreus) at a national primate center in 2009. Serological evidence of infection by TMAdV was also found in a human researcher at the facility and household family member, raising concerns for potential cross-species transmission of the virus. Here we present experimental evidence of cross-species TMAdV infection in common marmosets (Callithrix jacchus). Nasal inoculation of a cell cultured-adapted TMAdV strain into three marmosets produced an acute, mild respiratory illness characterized by low-grade fever, reduced activity, anorexia, and sneezing. An increase in virus-specific neutralization antibody titers accompanied the development of clinical signs. Although serially collected nasal swabs were positive for TMAdV for at least 8 days, all 3 infected marmosets spontaneously recovered by day 12 post-inoculation, and persistence of the virus in tissues could not be established. Thus, the pathogenesis of experimental inoculation of TMAdV in common marmosets resembled the mild, self-limiting respiratory infection typically seen in immunocompetent human hosts rather than the rapidly progressive, fatal pneumonia observed in 19 of 23 titi monkeys during the prior 2009 outbreak. These findings further establish the potential for adenovirus cross-species transmission and provide the basis for development of a monkey model useful for assessing the zoonotic potential of adenoviruses.

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TMAdV-Associated Pathology in Experimentally Infected Marmoset CJ29019.Histologic findings on necropsy tissue from day 15 post-inoculation include a mild bronchitis (A and B), enteritis (C and D), and an atypical nodular hyperplasia in the liver (E and F). The black rectangle in panels A, C, or E outlines the region magnified in panels B, D, or F, respectively.
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pone-0068558-g003: TMAdV-Associated Pathology in Experimentally Infected Marmoset CJ29019.Histologic findings on necropsy tissue from day 15 post-inoculation include a mild bronchitis (A and B), enteritis (C and D), and an atypical nodular hyperplasia in the liver (E and F). The black rectangle in panels A, C, or E outlines the region magnified in panels B, D, or F, respectively.

Mentions: No significant gross pathological lesions were observed. Only a few salient histologic findings were specific to TMAdV-inoculated animals (Table 2). A mild bronchitis (Fig. 3A–B) and focal area of atypical nodular hyperplasia of the liver (Fig. 3E–F) were observed in marmoset CJ29019 (Table 2). All 3 inoculated animals were found to have a mild enteritis (Fig. 3C–D) and/or colitis. Typical basophilic intranuclear inclusions consistent with active adenovirus infection were not observed in tissues from any of the TMAdV-infected marmosets. The paucity of histologic findings observed in marmosets experimentally infected with TMAdV is in sharp contrast with the striking lesions observed in moribund titi monkeys with TMAdV-associated pneumonia and hepatitis (Table 2) [6].


Experimental cross-species infection of common marmosets by titi monkey adenovirus.

Yu G, Yagi S, Carrion R, Chen EC, Liu M, Brasky KM, Lanford RE, Kelly KR, Bales KL, Schnurr DP, Canfield DR, Patterson JL, Chiu CY - PLoS ONE (2013)

TMAdV-Associated Pathology in Experimentally Infected Marmoset CJ29019.Histologic findings on necropsy tissue from day 15 post-inoculation include a mild bronchitis (A and B), enteritis (C and D), and an atypical nodular hyperplasia in the liver (E and F). The black rectangle in panels A, C, or E outlines the region magnified in panels B, D, or F, respectively.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3722195&req=5

pone-0068558-g003: TMAdV-Associated Pathology in Experimentally Infected Marmoset CJ29019.Histologic findings on necropsy tissue from day 15 post-inoculation include a mild bronchitis (A and B), enteritis (C and D), and an atypical nodular hyperplasia in the liver (E and F). The black rectangle in panels A, C, or E outlines the region magnified in panels B, D, or F, respectively.
Mentions: No significant gross pathological lesions were observed. Only a few salient histologic findings were specific to TMAdV-inoculated animals (Table 2). A mild bronchitis (Fig. 3A–B) and focal area of atypical nodular hyperplasia of the liver (Fig. 3E–F) were observed in marmoset CJ29019 (Table 2). All 3 inoculated animals were found to have a mild enteritis (Fig. 3C–D) and/or colitis. Typical basophilic intranuclear inclusions consistent with active adenovirus infection were not observed in tissues from any of the TMAdV-infected marmosets. The paucity of histologic findings observed in marmosets experimentally infected with TMAdV is in sharp contrast with the striking lesions observed in moribund titi monkeys with TMAdV-associated pneumonia and hepatitis (Table 2) [6].

Bottom Line: Here we present experimental evidence of cross-species TMAdV infection in common marmosets (Callithrix jacchus).Nasal inoculation of a cell cultured-adapted TMAdV strain into three marmosets produced an acute, mild respiratory illness characterized by low-grade fever, reduced activity, anorexia, and sneezing.An increase in virus-specific neutralization antibody titers accompanied the development of clinical signs.

View Article: PubMed Central - PubMed

Affiliation: Department of Laboratory Medicine, University of California San Francisco, San Francisco, California, United States of America.

ABSTRACT
Adenoviruses are DNA viruses that infect a number of vertebrate hosts and are associated with both sporadic and epidemic disease in humans. We previously identified a novel adenovirus, titi monkey adenovirus (TMAdV), as the cause of a fulminant pneumonia outbreak in a colony of titi monkeys (Callicebus cupreus) at a national primate center in 2009. Serological evidence of infection by TMAdV was also found in a human researcher at the facility and household family member, raising concerns for potential cross-species transmission of the virus. Here we present experimental evidence of cross-species TMAdV infection in common marmosets (Callithrix jacchus). Nasal inoculation of a cell cultured-adapted TMAdV strain into three marmosets produced an acute, mild respiratory illness characterized by low-grade fever, reduced activity, anorexia, and sneezing. An increase in virus-specific neutralization antibody titers accompanied the development of clinical signs. Although serially collected nasal swabs were positive for TMAdV for at least 8 days, all 3 infected marmosets spontaneously recovered by day 12 post-inoculation, and persistence of the virus in tissues could not be established. Thus, the pathogenesis of experimental inoculation of TMAdV in common marmosets resembled the mild, self-limiting respiratory infection typically seen in immunocompetent human hosts rather than the rapidly progressive, fatal pneumonia observed in 19 of 23 titi monkeys during the prior 2009 outbreak. These findings further establish the potential for adenovirus cross-species transmission and provide the basis for development of a monkey model useful for assessing the zoonotic potential of adenoviruses.

Show MeSH
Related in: MedlinePlus