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Differential expression patterns in chemosensory and non-chemosensory tissues of putative chemosensory genes identified by transcriptome analysis of insect pest the purple stem borer Sesamia inferens (Walker).

Zhang YN, Jin JY, Jin R, Xia YH, Zhou JJ, Deng JY, Dong SL - PLoS ONE (2013)

Bottom Line: Our results also revealed that some OR transcripts, such as the transcripts of SNMP2 and 2 IRs were expressed in non-chemosensory tissues, and some CSP transcripts were antenna-biased expression.Furthermore, no chemosensory transcript is specific to female sex pheromone gland and very few are found in the heads.The identification of a large set of putative chemosensory genes of each subfamily from a single insect species, together with their different expression profiles provide further information in understanding the functions of these chemosensory genes in S. inferens as well as other insects.

View Article: PubMed Central - PubMed

Affiliation: Education Ministry, Key Laboratory of Integrated Management of Crop Diseases and Pests, College of Plant Protection, Nanjing Agricultural University, Nanjing, China.

ABSTRACT

Background: A large number of insect chemosensory genes from different gene subfamilies have been identified and annotated, but their functional diversity and complexity are largely unknown. A systemic examination of expression patterns in chemosensory organs could provide important information.

Methodology/principal findings: We identified 92 putative chemosensory genes by analysing the transcriptome of the antennae and female sex pheromone gland of the purple stem borer Sesamia inferens, among them 87 are novel in this species, including 24 transcripts encoding for odorant binding proteins (OBPs), 24 for chemosensory proteins (CSPs), 2 for sensory neuron membrane proteins (SNMPs), 39 for odorant receptors (ORs) and 3 for ionotropic receptors (IRs). The transcriptome analyses were validated and quantified with a detailed global expression profiling by Reverse Transcription-PCR for all 92 transcripts and by Quantitative Real Time RT-PCR for selected 16 ones. Among the chemosensory gene subfamilies, CSP transcripts are most widely and evenly expressed in different tissues and stages, OBP transcripts showed a clear antenna bias and most of OR transcripts are only detected in adult antennae. Our results also revealed that some OR transcripts, such as the transcripts of SNMP2 and 2 IRs were expressed in non-chemosensory tissues, and some CSP transcripts were antenna-biased expression. Furthermore, no chemosensory transcript is specific to female sex pheromone gland and very few are found in the heads.

Conclusion: Our study revealed that there are a large number of chemosensory genes expressed in S. inferens, and some of them displayed unusual expression profile in non-chemosensory tissues. The identification of a large set of putative chemosensory genes of each subfamily from a single insect species, together with their different expression profiles provide further information in understanding the functions of these chemosensory genes in S. inferens as well as other insects.

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Phylogenetic tree of putative CSPs from S. inferens, M. sexta, S. littoralis and B. mori.The S. inferens translated unigenes are shown in blue. Accession numbers are given in Table S3. The tree was constructed with MEGA5.0, using the neighbour-joining method. Values indicated at the nodes are bootstrap values based on 1000 replicates, and the bootstrap values <50% are not shown. Sinf, Sesamia inferens; Msex, Manduca sexta; Slit, Spodoptera littoralis; Bmor, Bombyx mori.
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pone-0069715-g009: Phylogenetic tree of putative CSPs from S. inferens, M. sexta, S. littoralis and B. mori.The S. inferens translated unigenes are shown in blue. Accession numbers are given in Table S3. The tree was constructed with MEGA5.0, using the neighbour-joining method. Values indicated at the nodes are bootstrap values based on 1000 replicates, and the bootstrap values <50% are not shown. Sinf, Sesamia inferens; Msex, Manduca sexta; Slit, Spodoptera littoralis; Bmor, Bombyx mori.

Mentions: A phylogenetic tree of OBPs was constructed using protein sequences of the OBPs from S. inferens, M. sexta, S. littoralis and B. mori (Figure 8). It was shown that all PBP and GOBP sequences were clustered into distinct clades from other OBPs. More interestingly, the identified SinOBP sequences were clustered in each subclass (PBP1, PBP2, PBP3, GOBP1 and GOBP2) with at least one lepidopteran orthologue (Figure 8). Among the 24 putative CSPs, 20 sequences were clustered with at least one lepidopteran orthologous gene (Figure 9).


Differential expression patterns in chemosensory and non-chemosensory tissues of putative chemosensory genes identified by transcriptome analysis of insect pest the purple stem borer Sesamia inferens (Walker).

Zhang YN, Jin JY, Jin R, Xia YH, Zhou JJ, Deng JY, Dong SL - PLoS ONE (2013)

Phylogenetic tree of putative CSPs from S. inferens, M. sexta, S. littoralis and B. mori.The S. inferens translated unigenes are shown in blue. Accession numbers are given in Table S3. The tree was constructed with MEGA5.0, using the neighbour-joining method. Values indicated at the nodes are bootstrap values based on 1000 replicates, and the bootstrap values <50% are not shown. Sinf, Sesamia inferens; Msex, Manduca sexta; Slit, Spodoptera littoralis; Bmor, Bombyx mori.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3722147&req=5

pone-0069715-g009: Phylogenetic tree of putative CSPs from S. inferens, M. sexta, S. littoralis and B. mori.The S. inferens translated unigenes are shown in blue. Accession numbers are given in Table S3. The tree was constructed with MEGA5.0, using the neighbour-joining method. Values indicated at the nodes are bootstrap values based on 1000 replicates, and the bootstrap values <50% are not shown. Sinf, Sesamia inferens; Msex, Manduca sexta; Slit, Spodoptera littoralis; Bmor, Bombyx mori.
Mentions: A phylogenetic tree of OBPs was constructed using protein sequences of the OBPs from S. inferens, M. sexta, S. littoralis and B. mori (Figure 8). It was shown that all PBP and GOBP sequences were clustered into distinct clades from other OBPs. More interestingly, the identified SinOBP sequences were clustered in each subclass (PBP1, PBP2, PBP3, GOBP1 and GOBP2) with at least one lepidopteran orthologue (Figure 8). Among the 24 putative CSPs, 20 sequences were clustered with at least one lepidopteran orthologous gene (Figure 9).

Bottom Line: Our results also revealed that some OR transcripts, such as the transcripts of SNMP2 and 2 IRs were expressed in non-chemosensory tissues, and some CSP transcripts were antenna-biased expression.Furthermore, no chemosensory transcript is specific to female sex pheromone gland and very few are found in the heads.The identification of a large set of putative chemosensory genes of each subfamily from a single insect species, together with their different expression profiles provide further information in understanding the functions of these chemosensory genes in S. inferens as well as other insects.

View Article: PubMed Central - PubMed

Affiliation: Education Ministry, Key Laboratory of Integrated Management of Crop Diseases and Pests, College of Plant Protection, Nanjing Agricultural University, Nanjing, China.

ABSTRACT

Background: A large number of insect chemosensory genes from different gene subfamilies have been identified and annotated, but their functional diversity and complexity are largely unknown. A systemic examination of expression patterns in chemosensory organs could provide important information.

Methodology/principal findings: We identified 92 putative chemosensory genes by analysing the transcriptome of the antennae and female sex pheromone gland of the purple stem borer Sesamia inferens, among them 87 are novel in this species, including 24 transcripts encoding for odorant binding proteins (OBPs), 24 for chemosensory proteins (CSPs), 2 for sensory neuron membrane proteins (SNMPs), 39 for odorant receptors (ORs) and 3 for ionotropic receptors (IRs). The transcriptome analyses were validated and quantified with a detailed global expression profiling by Reverse Transcription-PCR for all 92 transcripts and by Quantitative Real Time RT-PCR for selected 16 ones. Among the chemosensory gene subfamilies, CSP transcripts are most widely and evenly expressed in different tissues and stages, OBP transcripts showed a clear antenna bias and most of OR transcripts are only detected in adult antennae. Our results also revealed that some OR transcripts, such as the transcripts of SNMP2 and 2 IRs were expressed in non-chemosensory tissues, and some CSP transcripts were antenna-biased expression. Furthermore, no chemosensory transcript is specific to female sex pheromone gland and very few are found in the heads.

Conclusion: Our study revealed that there are a large number of chemosensory genes expressed in S. inferens, and some of them displayed unusual expression profile in non-chemosensory tissues. The identification of a large set of putative chemosensory genes of each subfamily from a single insect species, together with their different expression profiles provide further information in understanding the functions of these chemosensory genes in S. inferens as well as other insects.

Show MeSH