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Exploring early micronutrient deficiencies in rainbow Trout (Oncorhynchus mykiss) by next-generation sequencing technology--from black box to functional genomics.

Olsvik PA, Hemre GI, Waagbø R - PLoS ONE (2013)

Bottom Line: This work studies final nutritional status and transcriptional responses of rainbow trout (Oncorhynchus mykiss Walbaum 1792) (28 g) after a 10 week feeding experiment designed to elucidate the effect of adding a vitamin and mineral premix on growth, health, and nutritional endpoints.Processes related to lipid metabolism, peptide hydrolysis, oxygen transportation, and growth development were mostly affected.Considering the transcriptomics data relative to changes in nutritional status from the feeding study and the background phenotypic outcome of growth performance and gill histopathology, the outcome of the transcriptional profiling are suggested to be mainly related to suboptimal pantothenic acid and vitamin C nutrition.

View Article: PubMed Central - PubMed

Affiliation: National Institute of Nutrition and Seafood Research (NIFES), Bergen, Norway. pal.olsvik@nifes.no

ABSTRACT
This work studies final nutritional status and transcriptional responses of rainbow trout (Oncorhynchus mykiss Walbaum 1792) (28 g) after a 10 week feeding experiment designed to elucidate the effect of adding a vitamin and mineral premix on growth, health, and nutritional endpoints. Juvenile fish were fed a either a diet supplemented with a vitamin and mineral premix (Diet S) or the same diet without premix supplementation (Diet U). The analyzed micronutrient composition of diets differed accordingly. Pooled livers from 15 fish from each dietary group were used to create suppression subtractive hybridization (SSH) cDNA libraries that were sequenced with 454 FLX GS Titanium Technology. In total 552 812 reads were sequenced from the two cDNA libraries. Ingenuity pathway analysis (IPA) was then used to characterize the hepatic transcriptome of the two dietary groups of rainbow trout. In the present communication we discuss how selected micronutrients may affect the transcriptome at suboptimal status by directly impacting the cellular metabolism, functions, and structures, and by introducing respective compensatory mechanisms. Processes related to lipid metabolism, peptide hydrolysis, oxygen transportation, and growth development were mostly affected. Considering the transcriptomics data relative to changes in nutritional status from the feeding study and the background phenotypic outcome of growth performance and gill histopathology, the outcome of the transcriptional profiling are suggested to be mainly related to suboptimal pantothenic acid and vitamin C nutrition.

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Related in: MedlinePlus

Gill histopathology observed epithelial hyperplasia in rainbow trout fed Diet U without vitamin and mineral premix for 10 weeks, as compared to normally appearing gills in fish fed the supplemented Diet S.(Photos: Dr. Anne-Berit Olsen, Norwegian Veterinary Institute, Bergen, Norway).
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pone-0069461-g001: Gill histopathology observed epithelial hyperplasia in rainbow trout fed Diet U without vitamin and mineral premix for 10 weeks, as compared to normally appearing gills in fish fed the supplemented Diet S.(Photos: Dr. Anne-Berit Olsen, Norwegian Veterinary Institute, Bergen, Norway).

Mentions: Gill histology performed on fish fed Diet U and Diet S from the final sampling showed mainly pathological changes in fish fed the unsupplemented diet, indicating a marginal micronutrient supply compared fish fed a complete diet. The histopathological changes included mononuclear infiltrations and epithelial hyperplasia (Figure 1). Some of the control fish fed Diet S showed minor bleedings/teleangiextasia, probably related to the sampling.


Exploring early micronutrient deficiencies in rainbow Trout (Oncorhynchus mykiss) by next-generation sequencing technology--from black box to functional genomics.

Olsvik PA, Hemre GI, Waagbø R - PLoS ONE (2013)

Gill histopathology observed epithelial hyperplasia in rainbow trout fed Diet U without vitamin and mineral premix for 10 weeks, as compared to normally appearing gills in fish fed the supplemented Diet S.(Photos: Dr. Anne-Berit Olsen, Norwegian Veterinary Institute, Bergen, Norway).
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3722103&req=5

pone-0069461-g001: Gill histopathology observed epithelial hyperplasia in rainbow trout fed Diet U without vitamin and mineral premix for 10 weeks, as compared to normally appearing gills in fish fed the supplemented Diet S.(Photos: Dr. Anne-Berit Olsen, Norwegian Veterinary Institute, Bergen, Norway).
Mentions: Gill histology performed on fish fed Diet U and Diet S from the final sampling showed mainly pathological changes in fish fed the unsupplemented diet, indicating a marginal micronutrient supply compared fish fed a complete diet. The histopathological changes included mononuclear infiltrations and epithelial hyperplasia (Figure 1). Some of the control fish fed Diet S showed minor bleedings/teleangiextasia, probably related to the sampling.

Bottom Line: This work studies final nutritional status and transcriptional responses of rainbow trout (Oncorhynchus mykiss Walbaum 1792) (28 g) after a 10 week feeding experiment designed to elucidate the effect of adding a vitamin and mineral premix on growth, health, and nutritional endpoints.Processes related to lipid metabolism, peptide hydrolysis, oxygen transportation, and growth development were mostly affected.Considering the transcriptomics data relative to changes in nutritional status from the feeding study and the background phenotypic outcome of growth performance and gill histopathology, the outcome of the transcriptional profiling are suggested to be mainly related to suboptimal pantothenic acid and vitamin C nutrition.

View Article: PubMed Central - PubMed

Affiliation: National Institute of Nutrition and Seafood Research (NIFES), Bergen, Norway. pal.olsvik@nifes.no

ABSTRACT
This work studies final nutritional status and transcriptional responses of rainbow trout (Oncorhynchus mykiss Walbaum 1792) (28 g) after a 10 week feeding experiment designed to elucidate the effect of adding a vitamin and mineral premix on growth, health, and nutritional endpoints. Juvenile fish were fed a either a diet supplemented with a vitamin and mineral premix (Diet S) or the same diet without premix supplementation (Diet U). The analyzed micronutrient composition of diets differed accordingly. Pooled livers from 15 fish from each dietary group were used to create suppression subtractive hybridization (SSH) cDNA libraries that were sequenced with 454 FLX GS Titanium Technology. In total 552 812 reads were sequenced from the two cDNA libraries. Ingenuity pathway analysis (IPA) was then used to characterize the hepatic transcriptome of the two dietary groups of rainbow trout. In the present communication we discuss how selected micronutrients may affect the transcriptome at suboptimal status by directly impacting the cellular metabolism, functions, and structures, and by introducing respective compensatory mechanisms. Processes related to lipid metabolism, peptide hydrolysis, oxygen transportation, and growth development were mostly affected. Considering the transcriptomics data relative to changes in nutritional status from the feeding study and the background phenotypic outcome of growth performance and gill histopathology, the outcome of the transcriptional profiling are suggested to be mainly related to suboptimal pantothenic acid and vitamin C nutrition.

Show MeSH
Related in: MedlinePlus