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The effects of Gamijinhae-tang on elastase/lipopolysaccharide-induced lung inflammation in an animal model of acute lung injury.

Sohn SH, Jang H, Kim Y, Jang YP, Cho SH, Jung H, Jung S, Bae H - BMC Complement Altern Med (2013)

Bottom Line: The therapeutic effect of GJHT is likely associated with its anti-inflammatory activity.The histological results also demonstrated the attenuation effect of GJHT on PPE- and LPS-induced lung inflammation.The results of this study indicate that GJHT has significantly reduces PPE- and LPS-induced lung inflammation.

View Article: PubMed Central - HTML - PubMed

ABSTRACT

Background: Gamijinhae-tang (GJHT) has long been used in Korea to treat respiratory diseases. The therapeutic effect of GJHT is likely associated with its anti-inflammatory activity. However, the precise mechanisms underlying its effects are unknown. This study was conducted to evaluate the protective effects of GJHT in a porcine pancreatic elastase (PPE) and lipopolysaccharide(LPS) induced animal model of acute lung injury (ALI).

Methods: In this study, mice were intranasally exposed to PPE and LPS for 4 weeks to induce chronic obstructive pulmonary disease (COPD)-like lung inflammation. Two hours prior to PPE and LPS administration, the treatment group was administered GJHT extracts via an oral injection. The numbers of neutrophils, lymphocytes, macrophages and total cells in the bronchoalveolar lavage (BAL) fluid were counted, and pro-inflammatory cytokines were also measured. For histologic analysis, hematoxylin and eosin (H&E) stains and periodic acid-Schiff (PAS) stains were evaluated.

Results: After inducing ALI by treating mice with PPE and LPS for 4 weeks, the numbers of neutrophils, lymphocytes and total cells were significantly lower in the GJHT group than in the ALI group. In addition, the IL-1β and IL-6 levels were significantly decreased in the GJHT group. The histological results also demonstrated the attenuation effect of GJHT on PPE- and LPS-induced lung inflammation.

Conclusions: The results of this study indicate that GJHT has significantly reduces PPE- and LPS-induced lung inflammation. The remarkable protective effects of GJHT suggest its therapeutic potential in COPD treatment.

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Related in: MedlinePlus

The effect of Gamijinhae-tang (GJHT) extract on goblet cell and musins. A) Mouse lung sections were stained with PAS (magnification × 400), B) goblet cell (%), and C) Muc5AC and Muc5B mRNA expression. Muc5AC and Muc5B mRNA expression were analyzed by quantitative real-time PCR. Expression levels of Muc5AC and Muc5B mRNA were normalized to GAPDH and expressed as the relative quantity to controls. Control: saline treated, ALI: PPE (porcine pancreatic elastase) + LPS (lipopolysaccaride) treated, ALI + Dexa: ALI + dexamethasone (Dexa), ALI + GJHT: ALI + GJHT (Gamijinhae-tang). Data are expressed as the mean ± S.E.M. (## p < 0.01 versus control and *p < 0.05, **p < 0.01 versus ALI; n = 5-7). The statistic power for Muc5AC; p = 0.0039, F = 7.89, and R2 = 0.76, and for Muc5B; p = 0.0042, F = 7.70, and R2 = 0.75.
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Figure 6: The effect of Gamijinhae-tang (GJHT) extract on goblet cell and musins. A) Mouse lung sections were stained with PAS (magnification × 400), B) goblet cell (%), and C) Muc5AC and Muc5B mRNA expression. Muc5AC and Muc5B mRNA expression were analyzed by quantitative real-time PCR. Expression levels of Muc5AC and Muc5B mRNA were normalized to GAPDH and expressed as the relative quantity to controls. Control: saline treated, ALI: PPE (porcine pancreatic elastase) + LPS (lipopolysaccaride) treated, ALI + Dexa: ALI + dexamethasone (Dexa), ALI + GJHT: ALI + GJHT (Gamijinhae-tang). Data are expressed as the mean ± S.E.M. (## p < 0.01 versus control and *p < 0.05, **p < 0.01 versus ALI; n = 5-7). The statistic power for Muc5AC; p = 0.0039, F = 7.89, and R2 = 0.76, and for Muc5B; p = 0.0042, F = 7.70, and R2 = 0.75.

Mentions: To evaluate the effects of GJHT on goblet cell hyperplasia in lung tissue, the number of PAS-positive cells was evaluated. The ALI group showed more PAS-positive goblet cells in the large airways. By contrast, dexamethasone-treated (1 mg/kg body wt) or GJHT-treated (100 or 300 mg/kg body wt) groups showed significantly fewer PAS-positive cells than ALI group (p < 0.0001, F = 15.94, and R2 = 0.76, Figure 5). PAS staining verification was performed via Muc5AC and Muc5B mRNA expression. The mucins Muc5AC and Muc5B are found at increased levels in ALI group. However, dexamethasone-treated (1 mg/kg body wt) or GJHT-treated (100 or 300 mg/kg body wt) groups are decreased expression than ALI group (Figure 6). These data suggest that treatment with GJHT had a powerful preventative effect on the induced chronic inflammatory lung disease.


The effects of Gamijinhae-tang on elastase/lipopolysaccharide-induced lung inflammation in an animal model of acute lung injury.

Sohn SH, Jang H, Kim Y, Jang YP, Cho SH, Jung H, Jung S, Bae H - BMC Complement Altern Med (2013)

The effect of Gamijinhae-tang (GJHT) extract on goblet cell and musins. A) Mouse lung sections were stained with PAS (magnification × 400), B) goblet cell (%), and C) Muc5AC and Muc5B mRNA expression. Muc5AC and Muc5B mRNA expression were analyzed by quantitative real-time PCR. Expression levels of Muc5AC and Muc5B mRNA were normalized to GAPDH and expressed as the relative quantity to controls. Control: saline treated, ALI: PPE (porcine pancreatic elastase) + LPS (lipopolysaccaride) treated, ALI + Dexa: ALI + dexamethasone (Dexa), ALI + GJHT: ALI + GJHT (Gamijinhae-tang). Data are expressed as the mean ± S.E.M. (## p < 0.01 versus control and *p < 0.05, **p < 0.01 versus ALI; n = 5-7). The statistic power for Muc5AC; p = 0.0039, F = 7.89, and R2 = 0.76, and for Muc5B; p = 0.0042, F = 7.70, and R2 = 0.75.
© Copyright Policy - open-access
Related In: Results  -  Collection

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Show All Figures
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Figure 6: The effect of Gamijinhae-tang (GJHT) extract on goblet cell and musins. A) Mouse lung sections were stained with PAS (magnification × 400), B) goblet cell (%), and C) Muc5AC and Muc5B mRNA expression. Muc5AC and Muc5B mRNA expression were analyzed by quantitative real-time PCR. Expression levels of Muc5AC and Muc5B mRNA were normalized to GAPDH and expressed as the relative quantity to controls. Control: saline treated, ALI: PPE (porcine pancreatic elastase) + LPS (lipopolysaccaride) treated, ALI + Dexa: ALI + dexamethasone (Dexa), ALI + GJHT: ALI + GJHT (Gamijinhae-tang). Data are expressed as the mean ± S.E.M. (## p < 0.01 versus control and *p < 0.05, **p < 0.01 versus ALI; n = 5-7). The statistic power for Muc5AC; p = 0.0039, F = 7.89, and R2 = 0.76, and for Muc5B; p = 0.0042, F = 7.70, and R2 = 0.75.
Mentions: To evaluate the effects of GJHT on goblet cell hyperplasia in lung tissue, the number of PAS-positive cells was evaluated. The ALI group showed more PAS-positive goblet cells in the large airways. By contrast, dexamethasone-treated (1 mg/kg body wt) or GJHT-treated (100 or 300 mg/kg body wt) groups showed significantly fewer PAS-positive cells than ALI group (p < 0.0001, F = 15.94, and R2 = 0.76, Figure 5). PAS staining verification was performed via Muc5AC and Muc5B mRNA expression. The mucins Muc5AC and Muc5B are found at increased levels in ALI group. However, dexamethasone-treated (1 mg/kg body wt) or GJHT-treated (100 or 300 mg/kg body wt) groups are decreased expression than ALI group (Figure 6). These data suggest that treatment with GJHT had a powerful preventative effect on the induced chronic inflammatory lung disease.

Bottom Line: The therapeutic effect of GJHT is likely associated with its anti-inflammatory activity.The histological results also demonstrated the attenuation effect of GJHT on PPE- and LPS-induced lung inflammation.The results of this study indicate that GJHT has significantly reduces PPE- and LPS-induced lung inflammation.

View Article: PubMed Central - HTML - PubMed

ABSTRACT

Background: Gamijinhae-tang (GJHT) has long been used in Korea to treat respiratory diseases. The therapeutic effect of GJHT is likely associated with its anti-inflammatory activity. However, the precise mechanisms underlying its effects are unknown. This study was conducted to evaluate the protective effects of GJHT in a porcine pancreatic elastase (PPE) and lipopolysaccharide(LPS) induced animal model of acute lung injury (ALI).

Methods: In this study, mice were intranasally exposed to PPE and LPS for 4 weeks to induce chronic obstructive pulmonary disease (COPD)-like lung inflammation. Two hours prior to PPE and LPS administration, the treatment group was administered GJHT extracts via an oral injection. The numbers of neutrophils, lymphocytes, macrophages and total cells in the bronchoalveolar lavage (BAL) fluid were counted, and pro-inflammatory cytokines were also measured. For histologic analysis, hematoxylin and eosin (H&E) stains and periodic acid-Schiff (PAS) stains were evaluated.

Results: After inducing ALI by treating mice with PPE and LPS for 4 weeks, the numbers of neutrophils, lymphocytes and total cells were significantly lower in the GJHT group than in the ALI group. In addition, the IL-1β and IL-6 levels were significantly decreased in the GJHT group. The histological results also demonstrated the attenuation effect of GJHT on PPE- and LPS-induced lung inflammation.

Conclusions: The results of this study indicate that GJHT has significantly reduces PPE- and LPS-induced lung inflammation. The remarkable protective effects of GJHT suggest its therapeutic potential in COPD treatment.

Show MeSH
Related in: MedlinePlus