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Unilateral or bilateral vagotomy induces ovulation in both ovaries of rats with polycystic ovarian syndrome.

Linares R, Hernández D, Morán C, Chavira R, Cárdenas M, Domínguez R, Morales-Ledesma L - Reprod. Biol. Endocrinol. (2013)

Bottom Line: Injecting estradiol valerate (EV) to pre-pubertal or adult female rat results in effects similar to those observed in women with polycystic ovarian syndrome (PCOS).The animals were sacrificed at 90-92 days of age, when they presented vaginal estrous preceded by a pro-estrus smear.The vagus nerve innervates the ovaries directly and indirectly through its synapsis in the celiac-superior-mesenteric ganglion, where the somas of neurons originating in the SON are located.

View Article: PubMed Central - HTML - PubMed

ABSTRACT

Background: Injecting estradiol valerate (EV) to pre-pubertal or adult female rat results in effects similar to those observed in women with polycystic ovarian syndrome (PCOS). One of the mechanisms involved in PCOS development is the hyperactivity of the sympathetic nervous system. In EV-induced PCOS rats, the unilateral sectioning of the superior ovarian nerve (SON) restores ovulation of the innervated ovary. This suggests that, in addition to the sympathetic innervation, other neural mechanisms are involved in the development/maintenance of PCOS. The aims of present study were analyze if the vagus nerve is one of the neural pathways participating in PCOS development.

Methods: Ten-day old rats were injected with EV dissolved in corn oil. At 24-days of age sham-surgery, unilateral, or bilateral sectioning of the vagus nerve (vagotomy) was performed on these rats. The animals were sacrificed at 90-92 days of age, when they presented vaginal estrous preceded by a pro-estrus smear.

Results: In EV-induced PCOS rats, unilateral or bilateral vagotomy restored ovulation in both ovaries. Follicle-stimulating hormone (FSH) levels in PCOS rats with unilateral or bilateral vagotomy were lower than in control rats.

Conclusions: This result suggests that in EV-induced PCOS rats the vagus nerve is a neural pathway participating in maintaining PCOS. The vagus nerve innervates the ovaries directly and indirectly through its synapsis in the celiac-superior-mesenteric ganglion, where the somas of neurons originating in the SON are located. Then, it is possible that vagotomy effects in EV-induced PCOS rats may be explained as a lack of communication between the central nervous system and the ovaries.

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Concentration of steroid hormones. P4 (A), T (B) and E2 (C) serum levels in rats with LSVN, RSVN or BSVN. Mean ± SEM of P4, T and E2 levels in rats injected with vehicle (Vh) or estradiol valerate (EV) at day 10 of life, untouched (control), with sham-surgery (sham) or unilateral (LSVN or RSVN) or bilateral vagotomy (BSVN) at day 24 of life, sacrificed at day 90–92 of life. a p < 0.05 vs. Vh control group, b p < 0.05 vs. paired Vh group, c p < 0.05 vs. sham Vh group; d p < 0.05 vs. EV control group; e p < 0.05 vs. sham EV group (Repeated Measures of analysis of variance, followed by Dunn’s test).
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Figure 3: Concentration of steroid hormones. P4 (A), T (B) and E2 (C) serum levels in rats with LSVN, RSVN or BSVN. Mean ± SEM of P4, T and E2 levels in rats injected with vehicle (Vh) or estradiol valerate (EV) at day 10 of life, untouched (control), with sham-surgery (sham) or unilateral (LSVN or RSVN) or bilateral vagotomy (BSVN) at day 24 of life, sacrificed at day 90–92 of life. a p < 0.05 vs. Vh control group, b p < 0.05 vs. paired Vh group, c p < 0.05 vs. sham Vh group; d p < 0.05 vs. EV control group; e p < 0.05 vs. sham EV group (Repeated Measures of analysis of variance, followed by Dunn’s test).

Mentions: P4 levels were similar in Vh-injected and EV-injected rats. Sham-surgery, RSVN or BSVN to Vh-injected rats resulted in higher P4 levels that Vh-injected control group. LSVN to Vh-injected rats resulted in lower P4 levels than in the Vh-injected sham-surgery group. Sham-surgery, RSVN or BSVN to EV-injected rats resulted in lower P4 levels than their respective Vh-injected group (Figure 3A).


Unilateral or bilateral vagotomy induces ovulation in both ovaries of rats with polycystic ovarian syndrome.

Linares R, Hernández D, Morán C, Chavira R, Cárdenas M, Domínguez R, Morales-Ledesma L - Reprod. Biol. Endocrinol. (2013)

Concentration of steroid hormones. P4 (A), T (B) and E2 (C) serum levels in rats with LSVN, RSVN or BSVN. Mean ± SEM of P4, T and E2 levels in rats injected with vehicle (Vh) or estradiol valerate (EV) at day 10 of life, untouched (control), with sham-surgery (sham) or unilateral (LSVN or RSVN) or bilateral vagotomy (BSVN) at day 24 of life, sacrificed at day 90–92 of life. a p < 0.05 vs. Vh control group, b p < 0.05 vs. paired Vh group, c p < 0.05 vs. sham Vh group; d p < 0.05 vs. EV control group; e p < 0.05 vs. sham EV group (Repeated Measures of analysis of variance, followed by Dunn’s test).
© Copyright Policy - open-access
Related In: Results  -  Collection

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Show All Figures
getmorefigures.php?uid=PMC3722028&req=5

Figure 3: Concentration of steroid hormones. P4 (A), T (B) and E2 (C) serum levels in rats with LSVN, RSVN or BSVN. Mean ± SEM of P4, T and E2 levels in rats injected with vehicle (Vh) or estradiol valerate (EV) at day 10 of life, untouched (control), with sham-surgery (sham) or unilateral (LSVN or RSVN) or bilateral vagotomy (BSVN) at day 24 of life, sacrificed at day 90–92 of life. a p < 0.05 vs. Vh control group, b p < 0.05 vs. paired Vh group, c p < 0.05 vs. sham Vh group; d p < 0.05 vs. EV control group; e p < 0.05 vs. sham EV group (Repeated Measures of analysis of variance, followed by Dunn’s test).
Mentions: P4 levels were similar in Vh-injected and EV-injected rats. Sham-surgery, RSVN or BSVN to Vh-injected rats resulted in higher P4 levels that Vh-injected control group. LSVN to Vh-injected rats resulted in lower P4 levels than in the Vh-injected sham-surgery group. Sham-surgery, RSVN or BSVN to EV-injected rats resulted in lower P4 levels than their respective Vh-injected group (Figure 3A).

Bottom Line: Injecting estradiol valerate (EV) to pre-pubertal or adult female rat results in effects similar to those observed in women with polycystic ovarian syndrome (PCOS).The animals were sacrificed at 90-92 days of age, when they presented vaginal estrous preceded by a pro-estrus smear.The vagus nerve innervates the ovaries directly and indirectly through its synapsis in the celiac-superior-mesenteric ganglion, where the somas of neurons originating in the SON are located.

View Article: PubMed Central - HTML - PubMed

ABSTRACT

Background: Injecting estradiol valerate (EV) to pre-pubertal or adult female rat results in effects similar to those observed in women with polycystic ovarian syndrome (PCOS). One of the mechanisms involved in PCOS development is the hyperactivity of the sympathetic nervous system. In EV-induced PCOS rats, the unilateral sectioning of the superior ovarian nerve (SON) restores ovulation of the innervated ovary. This suggests that, in addition to the sympathetic innervation, other neural mechanisms are involved in the development/maintenance of PCOS. The aims of present study were analyze if the vagus nerve is one of the neural pathways participating in PCOS development.

Methods: Ten-day old rats were injected with EV dissolved in corn oil. At 24-days of age sham-surgery, unilateral, or bilateral sectioning of the vagus nerve (vagotomy) was performed on these rats. The animals were sacrificed at 90-92 days of age, when they presented vaginal estrous preceded by a pro-estrus smear.

Results: In EV-induced PCOS rats, unilateral or bilateral vagotomy restored ovulation in both ovaries. Follicle-stimulating hormone (FSH) levels in PCOS rats with unilateral or bilateral vagotomy were lower than in control rats.

Conclusions: This result suggests that in EV-induced PCOS rats the vagus nerve is a neural pathway participating in maintaining PCOS. The vagus nerve innervates the ovaries directly and indirectly through its synapsis in the celiac-superior-mesenteric ganglion, where the somas of neurons originating in the SON are located. Then, it is possible that vagotomy effects in EV-induced PCOS rats may be explained as a lack of communication between the central nervous system and the ovaries.

Show MeSH
Related in: MedlinePlus