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The role of angiogenic, anti-angiogenic and vasoactive factors in pre-eclamptic African women: early- versus late-onset pre-eclampsia.

Govender L, Mackraj I, Gathiram P, Moodley J - Cardiovasc J Afr (2012)

Bottom Line: Serum sFlt-1 levels were significantly elevated and PlGF significantly reduced in early-onset PE compared to the normotensive group.Placental VEGF mRNA expression levels were significantly reduced in the late-onset preeclamptic group compared with the normotensives.The placental mRNA expression of AT1 receptor in the late-onset pre-eclamptic group was relatively raised compared to the normotensives, suggesting hypersensitivity to pressor agents.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Physiology and Physiological Chemistry, University of KwaZulu-Natal, Durban, South Africa.

ABSTRACT
The pathogenesis and aetiology of pre-eclampsia (PE) is still unclear. We investigated the role of angiogenic, antiangiogenic and vasoactive factors in black South African women with early- and late-onset PE. Serum soluble fms-like tyrosine kinase 1 (sFlt-1), soluble vascular endothelial growth factor (VEGF) and placental growth factor (PlGF) levels were determined using the ELISA technique, and placental mRNA expression levels of sFlt-1, VEGF, PlGF and AT1 receptors were determined using real-time PCR. Serum sFlt-1 levels were significantly elevated and PlGF significantly reduced in early-onset PE compared to the normotensive group. Placental VEGF mRNA expression levels were significantly reduced in the late-onset preeclamptic group compared with the normotensives. The placental mRNA expression of AT1 receptor in the late-onset pre-eclamptic group was relatively raised compared to the normotensives, suggesting hypersensitivity to pressor agents. We believe that the excess of serum sFlt-1 and reduced VEGF and PlGF levels favour an anti-angiogenic state and endothelial dysfunction leading to PE, and that the aetiology and pathogenesis of early- and late-onset PE differ.

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Placental mRNA expression levels of PlGF in normotensive (N) (n = 30), chronic hypertensive (CH) (n = 9), early-onset (EO) (n = 8) and late-onset (LO) (n = 9) pre-eclamptic groups.
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Figure 6: Placental mRNA expression levels of PlGF in normotensive (N) (n = 30), chronic hypertensive (CH) (n = 9), early-onset (EO) (n = 8) and late-onset (LO) (n = 9) pre-eclamptic groups.

Mentions: Although the differences in mRNA expression levels of PlGF among the four groups were not statistically significant, the late-onset pre-eclamptic group had the lowest level (0.81 ± 0.34) while the chronic hypertensive group had the highest level (2.17 ± 0.94) and the latter was 1.80-fold higher than in the normotensive group (1.205 ± 0.4818) (Fig. 6). The early-onset pre-eclamptic group (1.704 ± 0.5854) had a relatively higher level of PlGF expression level than the late-onset pre-eclamptic group but the level was lower than in the chronic hypertensive group.


The role of angiogenic, anti-angiogenic and vasoactive factors in pre-eclamptic African women: early- versus late-onset pre-eclampsia.

Govender L, Mackraj I, Gathiram P, Moodley J - Cardiovasc J Afr (2012)

Placental mRNA expression levels of PlGF in normotensive (N) (n = 30), chronic hypertensive (CH) (n = 9), early-onset (EO) (n = 8) and late-onset (LO) (n = 9) pre-eclamptic groups.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3721863&req=5

Figure 6: Placental mRNA expression levels of PlGF in normotensive (N) (n = 30), chronic hypertensive (CH) (n = 9), early-onset (EO) (n = 8) and late-onset (LO) (n = 9) pre-eclamptic groups.
Mentions: Although the differences in mRNA expression levels of PlGF among the four groups were not statistically significant, the late-onset pre-eclamptic group had the lowest level (0.81 ± 0.34) while the chronic hypertensive group had the highest level (2.17 ± 0.94) and the latter was 1.80-fold higher than in the normotensive group (1.205 ± 0.4818) (Fig. 6). The early-onset pre-eclamptic group (1.704 ± 0.5854) had a relatively higher level of PlGF expression level than the late-onset pre-eclamptic group but the level was lower than in the chronic hypertensive group.

Bottom Line: Serum sFlt-1 levels were significantly elevated and PlGF significantly reduced in early-onset PE compared to the normotensive group.Placental VEGF mRNA expression levels were significantly reduced in the late-onset preeclamptic group compared with the normotensives.The placental mRNA expression of AT1 receptor in the late-onset pre-eclamptic group was relatively raised compared to the normotensives, suggesting hypersensitivity to pressor agents.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Physiology and Physiological Chemistry, University of KwaZulu-Natal, Durban, South Africa.

ABSTRACT
The pathogenesis and aetiology of pre-eclampsia (PE) is still unclear. We investigated the role of angiogenic, antiangiogenic and vasoactive factors in black South African women with early- and late-onset PE. Serum soluble fms-like tyrosine kinase 1 (sFlt-1), soluble vascular endothelial growth factor (VEGF) and placental growth factor (PlGF) levels were determined using the ELISA technique, and placental mRNA expression levels of sFlt-1, VEGF, PlGF and AT1 receptors were determined using real-time PCR. Serum sFlt-1 levels were significantly elevated and PlGF significantly reduced in early-onset PE compared to the normotensive group. Placental VEGF mRNA expression levels were significantly reduced in the late-onset preeclamptic group compared with the normotensives. The placental mRNA expression of AT1 receptor in the late-onset pre-eclamptic group was relatively raised compared to the normotensives, suggesting hypersensitivity to pressor agents. We believe that the excess of serum sFlt-1 and reduced VEGF and PlGF levels favour an anti-angiogenic state and endothelial dysfunction leading to PE, and that the aetiology and pathogenesis of early- and late-onset PE differ.

Show MeSH
Related in: MedlinePlus