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The role of angiogenic, anti-angiogenic and vasoactive factors in pre-eclamptic African women: early- versus late-onset pre-eclampsia.

Govender L, Mackraj I, Gathiram P, Moodley J - Cardiovasc J Afr (2012)

Bottom Line: Serum sFlt-1 levels were significantly elevated and PlGF significantly reduced in early-onset PE compared to the normotensive group.Placental VEGF mRNA expression levels were significantly reduced in the late-onset preeclamptic group compared with the normotensives.The placental mRNA expression of AT1 receptor in the late-onset pre-eclamptic group was relatively raised compared to the normotensives, suggesting hypersensitivity to pressor agents.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Physiology and Physiological Chemistry, University of KwaZulu-Natal, Durban, South Africa.

ABSTRACT
The pathogenesis and aetiology of pre-eclampsia (PE) is still unclear. We investigated the role of angiogenic, antiangiogenic and vasoactive factors in black South African women with early- and late-onset PE. Serum soluble fms-like tyrosine kinase 1 (sFlt-1), soluble vascular endothelial growth factor (VEGF) and placental growth factor (PlGF) levels were determined using the ELISA technique, and placental mRNA expression levels of sFlt-1, VEGF, PlGF and AT1 receptors were determined using real-time PCR. Serum sFlt-1 levels were significantly elevated and PlGF significantly reduced in early-onset PE compared to the normotensive group. Placental VEGF mRNA expression levels were significantly reduced in the late-onset preeclamptic group compared with the normotensives. The placental mRNA expression of AT1 receptor in the late-onset pre-eclamptic group was relatively raised compared to the normotensives, suggesting hypersensitivity to pressor agents. We believe that the excess of serum sFlt-1 and reduced VEGF and PlGF levels favour an anti-angiogenic state and endothelial dysfunction leading to PE, and that the aetiology and pathogenesis of early- and late-onset PE differ.

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Placental mRNA expression levels of sFlt-1 in normotensive (N) (n = 30), chronic hypertensive (CH) (n = 9), early-onset (EO) (n = 8) and late-onset (LO) (n = 9) pre-eclamptic groups.
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Figure 3: Placental mRNA expression levels of sFlt-1 in normotensive (N) (n = 30), chronic hypertensive (CH) (n = 9), early-onset (EO) (n = 8) and late-onset (LO) (n = 9) pre-eclamptic groups.

Mentions: The highest mRNA expression level of sFlt-1 was found in the late-onset pre-eclamptic group (1.789 ± 0.513 fold) and the lowest level was found in the chronic hypertensive group (1.009 ± 0.162 fold) (Fig. 3). The level of sFlt-1 mRNA expression in the normotensive group (1.62 ± 0.24 fold) was slightly higher than that of the the early-onset pre-eclamptic group (1.401 ± 0.237 fold). There were no significant differences in the sFlt-1 concentrations among the four groups.


The role of angiogenic, anti-angiogenic and vasoactive factors in pre-eclamptic African women: early- versus late-onset pre-eclampsia.

Govender L, Mackraj I, Gathiram P, Moodley J - Cardiovasc J Afr (2012)

Placental mRNA expression levels of sFlt-1 in normotensive (N) (n = 30), chronic hypertensive (CH) (n = 9), early-onset (EO) (n = 8) and late-onset (LO) (n = 9) pre-eclamptic groups.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3721863&req=5

Figure 3: Placental mRNA expression levels of sFlt-1 in normotensive (N) (n = 30), chronic hypertensive (CH) (n = 9), early-onset (EO) (n = 8) and late-onset (LO) (n = 9) pre-eclamptic groups.
Mentions: The highest mRNA expression level of sFlt-1 was found in the late-onset pre-eclamptic group (1.789 ± 0.513 fold) and the lowest level was found in the chronic hypertensive group (1.009 ± 0.162 fold) (Fig. 3). The level of sFlt-1 mRNA expression in the normotensive group (1.62 ± 0.24 fold) was slightly higher than that of the the early-onset pre-eclamptic group (1.401 ± 0.237 fold). There were no significant differences in the sFlt-1 concentrations among the four groups.

Bottom Line: Serum sFlt-1 levels were significantly elevated and PlGF significantly reduced in early-onset PE compared to the normotensive group.Placental VEGF mRNA expression levels were significantly reduced in the late-onset preeclamptic group compared with the normotensives.The placental mRNA expression of AT1 receptor in the late-onset pre-eclamptic group was relatively raised compared to the normotensives, suggesting hypersensitivity to pressor agents.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Physiology and Physiological Chemistry, University of KwaZulu-Natal, Durban, South Africa.

ABSTRACT
The pathogenesis and aetiology of pre-eclampsia (PE) is still unclear. We investigated the role of angiogenic, antiangiogenic and vasoactive factors in black South African women with early- and late-onset PE. Serum soluble fms-like tyrosine kinase 1 (sFlt-1), soluble vascular endothelial growth factor (VEGF) and placental growth factor (PlGF) levels were determined using the ELISA technique, and placental mRNA expression levels of sFlt-1, VEGF, PlGF and AT1 receptors were determined using real-time PCR. Serum sFlt-1 levels were significantly elevated and PlGF significantly reduced in early-onset PE compared to the normotensive group. Placental VEGF mRNA expression levels were significantly reduced in the late-onset preeclamptic group compared with the normotensives. The placental mRNA expression of AT1 receptor in the late-onset pre-eclamptic group was relatively raised compared to the normotensives, suggesting hypersensitivity to pressor agents. We believe that the excess of serum sFlt-1 and reduced VEGF and PlGF levels favour an anti-angiogenic state and endothelial dysfunction leading to PE, and that the aetiology and pathogenesis of early- and late-onset PE differ.

Show MeSH
Related in: MedlinePlus