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Oral supplementation with areca-derived polyphenols attenuates food allergic responses in ovalbumin-sensitized mice.

Wang CC, Lin YR, Liao MH, Jan TR - BMC Complement Altern Med (2013)

Bottom Line: The serum level of OVA-specific IgE and the expression of interleukin-4 in the duodenum were suppressed by PANE treatment.In addition, PANE administration induced Gr-1+, IL-10+ and Gr-1+IL-10+ cells in the duodenum.These results demonstrate that oral intake of areca-derived polyphenols attenuates food allergic responses accompanied with a decreased Th2 immunity and an enhanced induction of functional myeloid-derived suppressor cells.

View Article: PubMed Central - HTML - PubMed

Affiliation: School of Pharmacy, Kaohsiung Medical University, Kaohsiung, Taiwan.

ABSTRACT

Background: Arecae semen, the dried slice of areca nuts, is a traditional Chinese medicine used to treat intestinal parasitosis, rectal tenesmus and diarrhea. Areca nuts contain a rich amount of polyphenols that have been shown to modulate the functionality of mast cells and T cells. The objective of this study is to investigate the effect of polyphenol-enriched areca nut extracts (PANE) against food allergy, a T cell-mediated immune disorder.

Methods: BALB/c mice were left untreated or administered with PANE (0.05% and 0.1%) via drinking water throughout the entire experiment. The mice were sensitized with ovalbumin (OVA) twice by intraperitoneal injection, and then repeatedly challenged with OVA by gavage to induce food allergic responses.

Results: PANE administration attenuated OVA-induced allergic responses, including the occurrence of diarrhea and the infiltration and degranulation of mast cells in the duodenum. The serum level of OVA-specific IgE and the expression of interleukin-4 in the duodenum were suppressed by PANE treatment. In addition, PANE administration induced Gr-1+, IL-10+ and Gr-1+IL-10+ cells in the duodenum.

Conclusion: These results demonstrate that oral intake of areca-derived polyphenols attenuates food allergic responses accompanied with a decreased Th2 immunity and an enhanced induction of functional myeloid-derived suppressor cells.

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Related in: MedlinePlus

PANE reduced the number of IL-4+ cells in the duodenum. Mice were treated as the protocol described in the Methods section. The expression of IL-4 in the duodenum was examined using IHC staining. Representative sections stained with IL-4 were shown (original magnification, x200). Cells with red signals around the blue nuclei indicate the IL-4 positive cells. Six measurements per section were counted. Data are expressed as the mean ± SE of 11 samples per group pooled from 3 independent experiments. *, p < 0.05 compared to the VH group.
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Figure 4: PANE reduced the number of IL-4+ cells in the duodenum. Mice were treated as the protocol described in the Methods section. The expression of IL-4 in the duodenum was examined using IHC staining. Representative sections stained with IL-4 were shown (original magnification, x200). Cells with red signals around the blue nuclei indicate the IL-4 positive cells. Six measurements per section were counted. Data are expressed as the mean ± SE of 11 samples per group pooled from 3 independent experiments. *, p < 0.05 compared to the VH group.

Mentions: We investigated whether PANE affected the expression of IL-4 in the duodenum of allergic mice. Results from IHC staining showed that PANE attenuated the expression of IL-4+ cells in the duodenum (Figure 4), whereas the number of IFN-γ+ cells was not altered (data not shown). To further address the potential mechanisms for ANE-mediated anti-allergic effects, we examined whether PANE affected the induction of myeloid-dericed suppressor cells (MDSC) in mice with food allergy. The number of Gr-1+, IL-10+ and Gr-1+IL-10+ cells was scarce in the NS and OVA groups. Oral intake of PANE significantly increased the number of Gr-1+, IL-10+ and Gr-1+IL-10+ cells in the duodenum, as compared to the VH control group (Figure 5; Table 3).


Oral supplementation with areca-derived polyphenols attenuates food allergic responses in ovalbumin-sensitized mice.

Wang CC, Lin YR, Liao MH, Jan TR - BMC Complement Altern Med (2013)

PANE reduced the number of IL-4+ cells in the duodenum. Mice were treated as the protocol described in the Methods section. The expression of IL-4 in the duodenum was examined using IHC staining. Representative sections stained with IL-4 were shown (original magnification, x200). Cells with red signals around the blue nuclei indicate the IL-4 positive cells. Six measurements per section were counted. Data are expressed as the mean ± SE of 11 samples per group pooled from 3 independent experiments. *, p < 0.05 compared to the VH group.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3717086&req=5

Figure 4: PANE reduced the number of IL-4+ cells in the duodenum. Mice were treated as the protocol described in the Methods section. The expression of IL-4 in the duodenum was examined using IHC staining. Representative sections stained with IL-4 were shown (original magnification, x200). Cells with red signals around the blue nuclei indicate the IL-4 positive cells. Six measurements per section were counted. Data are expressed as the mean ± SE of 11 samples per group pooled from 3 independent experiments. *, p < 0.05 compared to the VH group.
Mentions: We investigated whether PANE affected the expression of IL-4 in the duodenum of allergic mice. Results from IHC staining showed that PANE attenuated the expression of IL-4+ cells in the duodenum (Figure 4), whereas the number of IFN-γ+ cells was not altered (data not shown). To further address the potential mechanisms for ANE-mediated anti-allergic effects, we examined whether PANE affected the induction of myeloid-dericed suppressor cells (MDSC) in mice with food allergy. The number of Gr-1+, IL-10+ and Gr-1+IL-10+ cells was scarce in the NS and OVA groups. Oral intake of PANE significantly increased the number of Gr-1+, IL-10+ and Gr-1+IL-10+ cells in the duodenum, as compared to the VH control group (Figure 5; Table 3).

Bottom Line: The serum level of OVA-specific IgE and the expression of interleukin-4 in the duodenum were suppressed by PANE treatment.In addition, PANE administration induced Gr-1+, IL-10+ and Gr-1+IL-10+ cells in the duodenum.These results demonstrate that oral intake of areca-derived polyphenols attenuates food allergic responses accompanied with a decreased Th2 immunity and an enhanced induction of functional myeloid-derived suppressor cells.

View Article: PubMed Central - HTML - PubMed

Affiliation: School of Pharmacy, Kaohsiung Medical University, Kaohsiung, Taiwan.

ABSTRACT

Background: Arecae semen, the dried slice of areca nuts, is a traditional Chinese medicine used to treat intestinal parasitosis, rectal tenesmus and diarrhea. Areca nuts contain a rich amount of polyphenols that have been shown to modulate the functionality of mast cells and T cells. The objective of this study is to investigate the effect of polyphenol-enriched areca nut extracts (PANE) against food allergy, a T cell-mediated immune disorder.

Methods: BALB/c mice were left untreated or administered with PANE (0.05% and 0.1%) via drinking water throughout the entire experiment. The mice were sensitized with ovalbumin (OVA) twice by intraperitoneal injection, and then repeatedly challenged with OVA by gavage to induce food allergic responses.

Results: PANE administration attenuated OVA-induced allergic responses, including the occurrence of diarrhea and the infiltration and degranulation of mast cells in the duodenum. The serum level of OVA-specific IgE and the expression of interleukin-4 in the duodenum were suppressed by PANE treatment. In addition, PANE administration induced Gr-1+, IL-10+ and Gr-1+IL-10+ cells in the duodenum.

Conclusion: These results demonstrate that oral intake of areca-derived polyphenols attenuates food allergic responses accompanied with a decreased Th2 immunity and an enhanced induction of functional myeloid-derived suppressor cells.

Show MeSH
Related in: MedlinePlus