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Oral supplementation with areca-derived polyphenols attenuates food allergic responses in ovalbumin-sensitized mice.

Wang CC, Lin YR, Liao MH, Jan TR - BMC Complement Altern Med (2013)

Bottom Line: The serum level of OVA-specific IgE and the expression of interleukin-4 in the duodenum were suppressed by PANE treatment.In addition, PANE administration induced Gr-1+, IL-10+ and Gr-1+IL-10+ cells in the duodenum.These results demonstrate that oral intake of areca-derived polyphenols attenuates food allergic responses accompanied with a decreased Th2 immunity and an enhanced induction of functional myeloid-derived suppressor cells.

View Article: PubMed Central - HTML - PubMed

Affiliation: School of Pharmacy, Kaohsiung Medical University, Kaohsiung, Taiwan.

ABSTRACT

Background: Arecae semen, the dried slice of areca nuts, is a traditional Chinese medicine used to treat intestinal parasitosis, rectal tenesmus and diarrhea. Areca nuts contain a rich amount of polyphenols that have been shown to modulate the functionality of mast cells and T cells. The objective of this study is to investigate the effect of polyphenol-enriched areca nut extracts (PANE) against food allergy, a T cell-mediated immune disorder.

Methods: BALB/c mice were left untreated or administered with PANE (0.05% and 0.1%) via drinking water throughout the entire experiment. The mice were sensitized with ovalbumin (OVA) twice by intraperitoneal injection, and then repeatedly challenged with OVA by gavage to induce food allergic responses.

Results: PANE administration attenuated OVA-induced allergic responses, including the occurrence of diarrhea and the infiltration and degranulation of mast cells in the duodenum. The serum level of OVA-specific IgE and the expression of interleukin-4 in the duodenum were suppressed by PANE treatment. In addition, PANE administration induced Gr-1+, IL-10+ and Gr-1+IL-10+ cells in the duodenum.

Conclusion: These results demonstrate that oral intake of areca-derived polyphenols attenuates food allergic responses accompanied with a decreased Th2 immunity and an enhanced induction of functional myeloid-derived suppressor cells.

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Related in: MedlinePlus

PANE attenuated the infiltration of inflammatory cells and crypt hyperplasia in the duodenum of OVA-sensitized and challenged BALB/c mice. Mice were treated as the protocol described in the Methods section. Representative sections of the duodenum stained with H&E were shown (original magnification, ×100). Six measurements per section were counted and the villus/crypt ratio was measured. Data are expressed as mean ± SE of 11 mice per group pooled from 3 independent experiments. #, p < 0.05 compared to the NS group. *, p < 0.05 compared to the OVA group.
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Figure 2: PANE attenuated the infiltration of inflammatory cells and crypt hyperplasia in the duodenum of OVA-sensitized and challenged BALB/c mice. Mice were treated as the protocol described in the Methods section. Representative sections of the duodenum stained with H&E were shown (original magnification, ×100). Six measurements per section were counted and the villus/crypt ratio was measured. Data are expressed as mean ± SE of 11 mice per group pooled from 3 independent experiments. #, p < 0.05 compared to the NS group. *, p < 0.05 compared to the OVA group.

Mentions: Mice were treated with PANE as described in Figure 2. The spleen index was calculated as the spleen weight (mg) per body weight (g). Splenocytes were stained for CD4, CD8, B220 and CD11b and analyzed by flow cytometry. Data are expressed as mean ± SE of 11 samples per group pooled from 3 independent experiments.


Oral supplementation with areca-derived polyphenols attenuates food allergic responses in ovalbumin-sensitized mice.

Wang CC, Lin YR, Liao MH, Jan TR - BMC Complement Altern Med (2013)

PANE attenuated the infiltration of inflammatory cells and crypt hyperplasia in the duodenum of OVA-sensitized and challenged BALB/c mice. Mice were treated as the protocol described in the Methods section. Representative sections of the duodenum stained with H&E were shown (original magnification, ×100). Six measurements per section were counted and the villus/crypt ratio was measured. Data are expressed as mean ± SE of 11 mice per group pooled from 3 independent experiments. #, p < 0.05 compared to the NS group. *, p < 0.05 compared to the OVA group.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3717086&req=5

Figure 2: PANE attenuated the infiltration of inflammatory cells and crypt hyperplasia in the duodenum of OVA-sensitized and challenged BALB/c mice. Mice were treated as the protocol described in the Methods section. Representative sections of the duodenum stained with H&E were shown (original magnification, ×100). Six measurements per section were counted and the villus/crypt ratio was measured. Data are expressed as mean ± SE of 11 mice per group pooled from 3 independent experiments. #, p < 0.05 compared to the NS group. *, p < 0.05 compared to the OVA group.
Mentions: Mice were treated with PANE as described in Figure 2. The spleen index was calculated as the spleen weight (mg) per body weight (g). Splenocytes were stained for CD4, CD8, B220 and CD11b and analyzed by flow cytometry. Data are expressed as mean ± SE of 11 samples per group pooled from 3 independent experiments.

Bottom Line: The serum level of OVA-specific IgE and the expression of interleukin-4 in the duodenum were suppressed by PANE treatment.In addition, PANE administration induced Gr-1+, IL-10+ and Gr-1+IL-10+ cells in the duodenum.These results demonstrate that oral intake of areca-derived polyphenols attenuates food allergic responses accompanied with a decreased Th2 immunity and an enhanced induction of functional myeloid-derived suppressor cells.

View Article: PubMed Central - HTML - PubMed

Affiliation: School of Pharmacy, Kaohsiung Medical University, Kaohsiung, Taiwan.

ABSTRACT

Background: Arecae semen, the dried slice of areca nuts, is a traditional Chinese medicine used to treat intestinal parasitosis, rectal tenesmus and diarrhea. Areca nuts contain a rich amount of polyphenols that have been shown to modulate the functionality of mast cells and T cells. The objective of this study is to investigate the effect of polyphenol-enriched areca nut extracts (PANE) against food allergy, a T cell-mediated immune disorder.

Methods: BALB/c mice were left untreated or administered with PANE (0.05% and 0.1%) via drinking water throughout the entire experiment. The mice were sensitized with ovalbumin (OVA) twice by intraperitoneal injection, and then repeatedly challenged with OVA by gavage to induce food allergic responses.

Results: PANE administration attenuated OVA-induced allergic responses, including the occurrence of diarrhea and the infiltration and degranulation of mast cells in the duodenum. The serum level of OVA-specific IgE and the expression of interleukin-4 in the duodenum were suppressed by PANE treatment. In addition, PANE administration induced Gr-1+, IL-10+ and Gr-1+IL-10+ cells in the duodenum.

Conclusion: These results demonstrate that oral intake of areca-derived polyphenols attenuates food allergic responses accompanied with a decreased Th2 immunity and an enhanced induction of functional myeloid-derived suppressor cells.

Show MeSH
Related in: MedlinePlus