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In vitro antioxidant potential of dicliptera roxburghiana.

Ahmad B, Khan MR, Shah NA, Khan RA - BMC Complement Altern Med (2013)

Bottom Line: Stress caused by free radicals accumulation result into many hazardous diseases.In vitro lipid peroxidation and reducing power of the plant was also analyzed.All fractions except DRHF and DRAF possess strong reducing power ability and showed appreciable lipid peroxidation inhibition.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Biochemistry, Faculty of Biological Sciences, Quaid-i-Azam University, Islamabad 45320, Pakistan.

ABSTRACT

Background: Stress caused by free radicals accumulation result into many hazardous diseases. A number of investigations are focusing to find out the plant oriented natural antioxidant moieties. The basic aim of this research was to investigate the antioxidant potential, total Phenolic and flavonoids contents and photochemical screening of the crude methanol extract and its derived various fractions Dicliptera roxburghiana of Acanthaceae family.

Methods: Crude methanol extract of aerial parts of Dicliptera roxburghiana (DRME) was partitioned in to n-hexane (DRHF), chloroform (DRCF), ethyl acetate (DREF), n-butanol (DRBF) and the remaining soluble portion as residual aqueous fraction (DRAF). We evaluated the antioxidant activities of the extract and various fractions through different analytical methods such as DPPH, superoxide anion, ABTS, H2O2, hydroxyl radical and phosphomolybdate radical inhibition. In vitro lipid peroxidation and reducing power of the plant was also analyzed. Total flavonoid and phenolic contents of the extract and all fractions were also quantified. Plant was also subjected for preliminary phytochemical screening to confirm the presence or absence of various constituents in the plant.

Results: Phytochemical screening confirmed the presence of flavonoids, phenolics, tannins, alkaloids, saponins, terpenoids and coumarines. Quantitative analysis revealed the maximum amount of total phenolic and flavonoid contents in DRME while lowest in DRHF. Methanol extract, DREF, DRCF and DRBF exhibited promising antioxidant potential for DPPH, ABTS, H2O2, phosphomolybdate, superoxide anion and hydroxyl radical scavenging capabilities, while these were not appreciable for DRHF and DRAF. All fractions except DRHF and DRAF possess strong reducing power ability and showed appreciable lipid peroxidation inhibition.

Conclusion: These research investigations revealed that Dicliptera roxburghiana is a potent source of natural antioxidants. Hence the plant can be used for management of different stress and anxiety related ailments.

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Related in: MedlinePlus

Reducing power absorbance at 700 nm of different fractions of D. roxburghiana at different concentrations. M: methanol, H: n-hexane, E: ethyl acetate, C: chloroform, B: n-butanol, A: aqueous, A. Acid: ascorbic acid.
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Figure 3: Reducing power absorbance at 700 nm of different fractions of D. roxburghiana at different concentrations. M: methanol, H: n-hexane, E: ethyl acetate, C: chloroform, B: n-butanol, A: aqueous, A. Acid: ascorbic acid.

Mentions: As reducing power is a significant marker to measure the antioxidant capability so it was determined that increase in reducing power was actually the measure of antioxidant manifestations of the plant. Reducing power was also concentration dependant. Reducing power activity was not significant at low doses as compared to ascorbic acid but gradual increasing concentrations of fractions increased the reducing power capability of DRME, DRCF, DRBF and DREF significantly as demonstrated by Figure 3.


In vitro antioxidant potential of dicliptera roxburghiana.

Ahmad B, Khan MR, Shah NA, Khan RA - BMC Complement Altern Med (2013)

Reducing power absorbance at 700 nm of different fractions of D. roxburghiana at different concentrations. M: methanol, H: n-hexane, E: ethyl acetate, C: chloroform, B: n-butanol, A: aqueous, A. Acid: ascorbic acid.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3717081&req=5

Figure 3: Reducing power absorbance at 700 nm of different fractions of D. roxburghiana at different concentrations. M: methanol, H: n-hexane, E: ethyl acetate, C: chloroform, B: n-butanol, A: aqueous, A. Acid: ascorbic acid.
Mentions: As reducing power is a significant marker to measure the antioxidant capability so it was determined that increase in reducing power was actually the measure of antioxidant manifestations of the plant. Reducing power was also concentration dependant. Reducing power activity was not significant at low doses as compared to ascorbic acid but gradual increasing concentrations of fractions increased the reducing power capability of DRME, DRCF, DRBF and DREF significantly as demonstrated by Figure 3.

Bottom Line: Stress caused by free radicals accumulation result into many hazardous diseases.In vitro lipid peroxidation and reducing power of the plant was also analyzed.All fractions except DRHF and DRAF possess strong reducing power ability and showed appreciable lipid peroxidation inhibition.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Biochemistry, Faculty of Biological Sciences, Quaid-i-Azam University, Islamabad 45320, Pakistan.

ABSTRACT

Background: Stress caused by free radicals accumulation result into many hazardous diseases. A number of investigations are focusing to find out the plant oriented natural antioxidant moieties. The basic aim of this research was to investigate the antioxidant potential, total Phenolic and flavonoids contents and photochemical screening of the crude methanol extract and its derived various fractions Dicliptera roxburghiana of Acanthaceae family.

Methods: Crude methanol extract of aerial parts of Dicliptera roxburghiana (DRME) was partitioned in to n-hexane (DRHF), chloroform (DRCF), ethyl acetate (DREF), n-butanol (DRBF) and the remaining soluble portion as residual aqueous fraction (DRAF). We evaluated the antioxidant activities of the extract and various fractions through different analytical methods such as DPPH, superoxide anion, ABTS, H2O2, hydroxyl radical and phosphomolybdate radical inhibition. In vitro lipid peroxidation and reducing power of the plant was also analyzed. Total flavonoid and phenolic contents of the extract and all fractions were also quantified. Plant was also subjected for preliminary phytochemical screening to confirm the presence or absence of various constituents in the plant.

Results: Phytochemical screening confirmed the presence of flavonoids, phenolics, tannins, alkaloids, saponins, terpenoids and coumarines. Quantitative analysis revealed the maximum amount of total phenolic and flavonoid contents in DRME while lowest in DRHF. Methanol extract, DREF, DRCF and DRBF exhibited promising antioxidant potential for DPPH, ABTS, H2O2, phosphomolybdate, superoxide anion and hydroxyl radical scavenging capabilities, while these were not appreciable for DRHF and DRAF. All fractions except DRHF and DRAF possess strong reducing power ability and showed appreciable lipid peroxidation inhibition.

Conclusion: These research investigations revealed that Dicliptera roxburghiana is a potent source of natural antioxidants. Hence the plant can be used for management of different stress and anxiety related ailments.

Show MeSH
Related in: MedlinePlus