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The characteristics of oviposition and hormonal and gene regulation of ovarian follicle development in Magang geese.

Qin Q, Sun A, Guo R, Lei M, Ying S, Shi Z - Reprod. Biol. Endocrinol. (2013)

Bottom Line: LHR expression levels increased from the small to the large mature follicles, but FSHR expression decreased in the granulosa and thecal layers.During the oviposition cycle, plasma concentrations of gonadal hormones decreased rapidly, whereas the level of PGFM peaked around ovulation.The rapid rebound of post-ovulatory secretions of inhibin and follistatin may inhibit recruitment of new SYF recruitment once a sequence of eggs is started, and may limit the egg clutch size to no more than the number of LYFs present before the first sequence egg.

View Article: PubMed Central - HTML - PubMed

ABSTRACT

Background: Egg laying in Magang geese is characterized by extended interruption between clutches and lowing laying rate. Both the ovarian follicular development and ovulation characteristics, and the associated endocrine and molecular regulatory mechanisms involved are poorly understood, but could be important for guiding development of molecule aided selection of egg laying performances in geese. This study, therefore, recorded egg-laying characteristics of Magang geese, and the endocrine and molecular regulatory mechanisms of ovarian follicular development, maturation, and ovulation in Magang geese.

Methods: Oviposition, ovarian follicle development, and reproductive hormone and gene expression profiles were observed in a small flock of Magang geese.

Results: Greater than 73% of eggs were laid during the day. The average oviposition interval was 46.8 h (36-55 h). It took approximately 18 days for large white follicles to develop into mature F1 follicles; follicular growth was exponential. LHR expression levels increased from the small to the large mature follicles, but FSHR expression decreased in the granulosa and thecal layers. As the follicles matured, inhibin alpha and inhibin betaA expression increased in the granulosa layer. Activin IR, activin IIRA, activin IIRB, and beta-glycan expressions also increased as the follicles increased in size, but were more abundantly expressed in the thecal than in the granulosa layers. During the oviposition cycle, plasma concentrations of gonadal hormones decreased rapidly, whereas the level of PGFM peaked around ovulation. The profiles of activin, inhibin, follistatin, estradiol, and progesterone leading to ovulation were characterized.

Conclusions: The molecular and endocrine mechanisms that regulate follicular development in Magang geese are similar to those in chickens. Moreover, gonadotropin regulation and interaction between activin, inhibin, and follistatin secretion may govern 3-stage maturation in the final preovulatory follicles in Magang geese. The rapid rebound of post-ovulatory secretions of inhibin and follistatin may inhibit recruitment of new SYF recruitment once a sequence of eggs is started, and may limit the egg clutch size to no more than the number of LYFs present before the first sequence egg.

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Plasma concentrations of PGFM (A), P4 (B), E2 (C), activin A (D), inhibin (E) and follistatin (F) during the oviposition or ovulation cycle. Vertical bars represent the standard errors of the means. The arrow depicts the time of oviposition. Values marked with different letters were significantly different (a–b: P < 0.05; A–B, A–C, B-C: P < 0.01).
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Figure 3: Plasma concentrations of PGFM (A), P4 (B), E2 (C), activin A (D), inhibin (E) and follistatin (F) during the oviposition or ovulation cycle. Vertical bars represent the standard errors of the means. The arrow depicts the time of oviposition. Values marked with different letters were significantly different (a–b: P < 0.05; A–B, A–C, B-C: P < 0.01).

Mentions: Within the 48-h blood sampling period, the most significant change in plasma pgFM was a single abrupt peak that corresponded to oviposition. From the intermediate levels of 2 pg/mL at 1.5 h before oviposition, plasma PGFM increased abruptly to a 5 pg/mL peak 0.5 h before oviposition. After 2 h, the concentration returned to basal levels (Figure 3A). The concentrations remained low until 28 h, at which point they rose slightly to form a second marginal increase from 4 h to 1.5 h before the next oviposition.


The characteristics of oviposition and hormonal and gene regulation of ovarian follicle development in Magang geese.

Qin Q, Sun A, Guo R, Lei M, Ying S, Shi Z - Reprod. Biol. Endocrinol. (2013)

Plasma concentrations of PGFM (A), P4 (B), E2 (C), activin A (D), inhibin (E) and follistatin (F) during the oviposition or ovulation cycle. Vertical bars represent the standard errors of the means. The arrow depicts the time of oviposition. Values marked with different letters were significantly different (a–b: P < 0.05; A–B, A–C, B-C: P < 0.01).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3716988&req=5

Figure 3: Plasma concentrations of PGFM (A), P4 (B), E2 (C), activin A (D), inhibin (E) and follistatin (F) during the oviposition or ovulation cycle. Vertical bars represent the standard errors of the means. The arrow depicts the time of oviposition. Values marked with different letters were significantly different (a–b: P < 0.05; A–B, A–C, B-C: P < 0.01).
Mentions: Within the 48-h blood sampling period, the most significant change in plasma pgFM was a single abrupt peak that corresponded to oviposition. From the intermediate levels of 2 pg/mL at 1.5 h before oviposition, plasma PGFM increased abruptly to a 5 pg/mL peak 0.5 h before oviposition. After 2 h, the concentration returned to basal levels (Figure 3A). The concentrations remained low until 28 h, at which point they rose slightly to form a second marginal increase from 4 h to 1.5 h before the next oviposition.

Bottom Line: LHR expression levels increased from the small to the large mature follicles, but FSHR expression decreased in the granulosa and thecal layers.During the oviposition cycle, plasma concentrations of gonadal hormones decreased rapidly, whereas the level of PGFM peaked around ovulation.The rapid rebound of post-ovulatory secretions of inhibin and follistatin may inhibit recruitment of new SYF recruitment once a sequence of eggs is started, and may limit the egg clutch size to no more than the number of LYFs present before the first sequence egg.

View Article: PubMed Central - HTML - PubMed

ABSTRACT

Background: Egg laying in Magang geese is characterized by extended interruption between clutches and lowing laying rate. Both the ovarian follicular development and ovulation characteristics, and the associated endocrine and molecular regulatory mechanisms involved are poorly understood, but could be important for guiding development of molecule aided selection of egg laying performances in geese. This study, therefore, recorded egg-laying characteristics of Magang geese, and the endocrine and molecular regulatory mechanisms of ovarian follicular development, maturation, and ovulation in Magang geese.

Methods: Oviposition, ovarian follicle development, and reproductive hormone and gene expression profiles were observed in a small flock of Magang geese.

Results: Greater than 73% of eggs were laid during the day. The average oviposition interval was 46.8 h (36-55 h). It took approximately 18 days for large white follicles to develop into mature F1 follicles; follicular growth was exponential. LHR expression levels increased from the small to the large mature follicles, but FSHR expression decreased in the granulosa and thecal layers. As the follicles matured, inhibin alpha and inhibin betaA expression increased in the granulosa layer. Activin IR, activin IIRA, activin IIRB, and beta-glycan expressions also increased as the follicles increased in size, but were more abundantly expressed in the thecal than in the granulosa layers. During the oviposition cycle, plasma concentrations of gonadal hormones decreased rapidly, whereas the level of PGFM peaked around ovulation. The profiles of activin, inhibin, follistatin, estradiol, and progesterone leading to ovulation were characterized.

Conclusions: The molecular and endocrine mechanisms that regulate follicular development in Magang geese are similar to those in chickens. Moreover, gonadotropin regulation and interaction between activin, inhibin, and follistatin secretion may govern 3-stage maturation in the final preovulatory follicles in Magang geese. The rapid rebound of post-ovulatory secretions of inhibin and follistatin may inhibit recruitment of new SYF recruitment once a sequence of eggs is started, and may limit the egg clutch size to no more than the number of LYFs present before the first sequence egg.

Show MeSH