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Preparation of hierarchical mesoporous CaCO3 by a facile binary solvent approach as anticancer drug carrier for etoposide.

Peng H, Li K, Wang T, Wang J, Wang J, Zhu R, Sun D, Wang S - Nanoscale Res Lett (2013)

Bottom Line: MTT assay showed that compared with free etoposide, ECCNSs exhibited a higher cell inhibition ratio against SGC-7901 cells and also decreased the toxicity of etoposide to HEK 293 T cells.The apoptosis test revealed that etoposide entrapped in CCNSs could enhance the delivery efficiencies of drug to achieve an improved inhibition effect on cell growth.These results clearly implied that the CCNSs are a promising drug delivery system for etoposide in cancer therapy.

View Article: PubMed Central - HTML - PubMed

Affiliation: School of Life Science and Technology, Tongji University, 1239 Siping Road, Shanghai 200092, People's Republic of China. sundongmei@tongji.edu.cn.

ABSTRACT
To develop a nontoxic system for targeting therapy, a new highly ordered hierarchical mesoporous calcium carbonate nanospheres (CCNSs) as small drug carriers has been synthesized by a mild and facile binary solvent approach under the normal temperature and pressure. The hierarchical structure by multistage self-assembled strategy was confirmed by TEM and SEM, and a possible formation process was proposed. Due to the large fraction of voids inside the nanospheres which provides space for physical absorption, the CCNSs can stably encapsulate the anticancer drug etoposide with the drug loading efficiency as high as 39.7 wt.%, and etoposide-loaded CCNS (ECCNS) nanoparticles can dispersed well in the cell culture. Besides, the drug release behavior investigated at three different pH values showed that the release of etoposide from CCNSs was pH-sensitive. MTT assay showed that compared with free etoposide, ECCNSs exhibited a higher cell inhibition ratio against SGC-7901 cells and also decreased the toxicity of etoposide to HEK 293 T cells. The CLSM image showed that ECCNSs exhibited a high efficiency of intracellular delivery, especially in nuclear invasion. The apoptosis test revealed that etoposide entrapped in CCNSs could enhance the delivery efficiencies of drug to achieve an improved inhibition effect on cell growth. These results clearly implied that the CCNSs are a promising drug delivery system for etoposide in cancer therapy.

No MeSH data available.


Related in: MedlinePlus

A representative illustration of ECCNSs targeting.
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Figure 11: A representative illustration of ECCNSs targeting.

Mentions: Referring to some previous reports [51,52], the possible mechanism for the targeted delivery of the ECCNSs is illustrated in FigureĀ 11. Most of the biodegradable ECCNSs decompose into the secondary nanoparticles in the vicinity of the tumor endothelium, with the release of epotoside. The small therapeutic nanoparticles and drugs readily pass through the endothelia into tumor tissues for efficient permeability [53]. The degradation of the materials in the endosomes or lysosomes of tumor cells may determine the almost exclusive internalization along clathrin-coated pits pathway. The multistage decomposition of ECCNSs in blood vessels or tumor tissue is likely to play a key role in determining their targeting and biological activity [54].


Preparation of hierarchical mesoporous CaCO3 by a facile binary solvent approach as anticancer drug carrier for etoposide.

Peng H, Li K, Wang T, Wang J, Wang J, Zhu R, Sun D, Wang S - Nanoscale Res Lett (2013)

A representative illustration of ECCNSs targeting.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3716939&req=5

Figure 11: A representative illustration of ECCNSs targeting.
Mentions: Referring to some previous reports [51,52], the possible mechanism for the targeted delivery of the ECCNSs is illustrated in FigureĀ 11. Most of the biodegradable ECCNSs decompose into the secondary nanoparticles in the vicinity of the tumor endothelium, with the release of epotoside. The small therapeutic nanoparticles and drugs readily pass through the endothelia into tumor tissues for efficient permeability [53]. The degradation of the materials in the endosomes or lysosomes of tumor cells may determine the almost exclusive internalization along clathrin-coated pits pathway. The multistage decomposition of ECCNSs in blood vessels or tumor tissue is likely to play a key role in determining their targeting and biological activity [54].

Bottom Line: MTT assay showed that compared with free etoposide, ECCNSs exhibited a higher cell inhibition ratio against SGC-7901 cells and also decreased the toxicity of etoposide to HEK 293 T cells.The apoptosis test revealed that etoposide entrapped in CCNSs could enhance the delivery efficiencies of drug to achieve an improved inhibition effect on cell growth.These results clearly implied that the CCNSs are a promising drug delivery system for etoposide in cancer therapy.

View Article: PubMed Central - HTML - PubMed

Affiliation: School of Life Science and Technology, Tongji University, 1239 Siping Road, Shanghai 200092, People's Republic of China. sundongmei@tongji.edu.cn.

ABSTRACT
To develop a nontoxic system for targeting therapy, a new highly ordered hierarchical mesoporous calcium carbonate nanospheres (CCNSs) as small drug carriers has been synthesized by a mild and facile binary solvent approach under the normal temperature and pressure. The hierarchical structure by multistage self-assembled strategy was confirmed by TEM and SEM, and a possible formation process was proposed. Due to the large fraction of voids inside the nanospheres which provides space for physical absorption, the CCNSs can stably encapsulate the anticancer drug etoposide with the drug loading efficiency as high as 39.7 wt.%, and etoposide-loaded CCNS (ECCNS) nanoparticles can dispersed well in the cell culture. Besides, the drug release behavior investigated at three different pH values showed that the release of etoposide from CCNSs was pH-sensitive. MTT assay showed that compared with free etoposide, ECCNSs exhibited a higher cell inhibition ratio against SGC-7901 cells and also decreased the toxicity of etoposide to HEK 293 T cells. The CLSM image showed that ECCNSs exhibited a high efficiency of intracellular delivery, especially in nuclear invasion. The apoptosis test revealed that etoposide entrapped in CCNSs could enhance the delivery efficiencies of drug to achieve an improved inhibition effect on cell growth. These results clearly implied that the CCNSs are a promising drug delivery system for etoposide in cancer therapy.

No MeSH data available.


Related in: MedlinePlus