Limits...
A nebulized complex traditional Chinese medicine inhibits Histamine and IL-4 production by ovalbumin in guinea pigs and can stabilize mast cells in vitro.

Chang HC, Gong CC, Chan CL, Mak OT - BMC Complement Altern Med (2013)

Bottom Line: Traditional Chinese medicines have been used for anti-asthma treatment for several centuries in many Asian countries, and have been shown to effectively relieve symptoms.We found that the inhibition of bronchoconstriction by the CTCM was attenuated by pretreatment with propranolol, suggesting that the CTCM has a bronchodilator effect that is associated with beta-adrenergic receptor.In conclusion, our results suggested that the CTCM is a kind of bronchodilator and also a mast cell stabilizer.

View Article: PubMed Central - HTML - PubMed

ABSTRACT

Background: Traditional Chinese medicines have been used for anti-asthma treatment for several centuries in many Asian countries, and have been shown to effectively relieve symptoms. Our previous study demonstrated that a complex traditional Chinese medicine (CTCM) administered in nebulized form through the intratracheal route is effective against early-phase air-flow obstruction and can inhibit IL-5 production in ovalbumin (OVA)-sensitized guinea pigs. However, the antiasthmatic mechanisms of CTCMs are still unclear.

Methods: In this study, we examined the underlying mechanism of a CTCM that we used in our previous study in order to ascertain its function in the early-phase response to OVA challenge.

Results: We found that the inhibition of bronchoconstriction by the CTCM was attenuated by pretreatment with propranolol, suggesting that the CTCM has a bronchodilator effect that is associated with beta-adrenergic receptor. Our results also showed that the CTCM inhibited histamine and IL-4 secretion in the OVA-induced airway hypersensitivity in guinea pigs at 5 min post-OVA challenge, and in vitro study revealed that the CTCM is able to stabilize mast cells.

Conclusion: In conclusion, our results suggested that the CTCM is a kind of bronchodilator and also a mast cell stabilizer. Our findings provide useful information regarding the possible mechanism of the CTCM, and show its potential for application in the treatment of allergenic airway disease.

Show MeSH

Related in: MedlinePlus

Effect of the CTCM on the release of histamine and IL-4 in P815 mast cells. Compound 48/80 was used to activate the P815 mast cells as a positive control, and cromolyn sodium was used to stabilize the mast cells as a negative control. The release of (A) histamine and (B) IL-4 in the culture medium with saline, “Compound 48/80 (C48/80) + CTCM (100 μg/ml)” or “Compound 48/80 + cromolyn sodium” were examined (one-way ANOVA with a Duncan post-hoc test **P <0.01; compared to Compound 48/80 treatment).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC3716888&req=5

Figure 5: Effect of the CTCM on the release of histamine and IL-4 in P815 mast cells. Compound 48/80 was used to activate the P815 mast cells as a positive control, and cromolyn sodium was used to stabilize the mast cells as a negative control. The release of (A) histamine and (B) IL-4 in the culture medium with saline, “Compound 48/80 (C48/80) + CTCM (100 μg/ml)” or “Compound 48/80 + cromolyn sodium” were examined (one-way ANOVA with a Duncan post-hoc test **P <0.01; compared to Compound 48/80 treatment).

Mentions: The baseline value for histamine release in the P815 cells was 0.29 ng/ml. No significant change was seen when the unactivated P815 cells were treated with CTCM alone. In the cells activated with Compound 48/80, the histamine release was increased to 9.06 ng/ml. Interestingly, when P815 cells were pretreated with the CTCM followed by Compound 48/80, the results showed a 5-fold decrease in histamine release. The inhibition ability of the CTCM was close to that of cromolyn sodium, a mast cell stabilizer (Figure 5A). On the other hand, Compound 48/80 treatment induced IL-4 release from a baseline of 53.10 pg/ml to 218.48 pg/ml (Figure 5B). Although the CTCM alone did not affect IL-4 release in the unactivated cells, CTCM attenuated the IL-4 level to 93.52 pg/ml in the Compound 48/80-activated mast cells, which was close to the inhibition effect (reduced to (86.24 pg/ml) of cromolyn sodium pre-treatment (Figure 5B).


A nebulized complex traditional Chinese medicine inhibits Histamine and IL-4 production by ovalbumin in guinea pigs and can stabilize mast cells in vitro.

Chang HC, Gong CC, Chan CL, Mak OT - BMC Complement Altern Med (2013)

Effect of the CTCM on the release of histamine and IL-4 in P815 mast cells. Compound 48/80 was used to activate the P815 mast cells as a positive control, and cromolyn sodium was used to stabilize the mast cells as a negative control. The release of (A) histamine and (B) IL-4 in the culture medium with saline, “Compound 48/80 (C48/80) + CTCM (100 μg/ml)” or “Compound 48/80 + cromolyn sodium” were examined (one-way ANOVA with a Duncan post-hoc test **P <0.01; compared to Compound 48/80 treatment).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3716888&req=5

Figure 5: Effect of the CTCM on the release of histamine and IL-4 in P815 mast cells. Compound 48/80 was used to activate the P815 mast cells as a positive control, and cromolyn sodium was used to stabilize the mast cells as a negative control. The release of (A) histamine and (B) IL-4 in the culture medium with saline, “Compound 48/80 (C48/80) + CTCM (100 μg/ml)” or “Compound 48/80 + cromolyn sodium” were examined (one-way ANOVA with a Duncan post-hoc test **P <0.01; compared to Compound 48/80 treatment).
Mentions: The baseline value for histamine release in the P815 cells was 0.29 ng/ml. No significant change was seen when the unactivated P815 cells were treated with CTCM alone. In the cells activated with Compound 48/80, the histamine release was increased to 9.06 ng/ml. Interestingly, when P815 cells were pretreated with the CTCM followed by Compound 48/80, the results showed a 5-fold decrease in histamine release. The inhibition ability of the CTCM was close to that of cromolyn sodium, a mast cell stabilizer (Figure 5A). On the other hand, Compound 48/80 treatment induced IL-4 release from a baseline of 53.10 pg/ml to 218.48 pg/ml (Figure 5B). Although the CTCM alone did not affect IL-4 release in the unactivated cells, CTCM attenuated the IL-4 level to 93.52 pg/ml in the Compound 48/80-activated mast cells, which was close to the inhibition effect (reduced to (86.24 pg/ml) of cromolyn sodium pre-treatment (Figure 5B).

Bottom Line: Traditional Chinese medicines have been used for anti-asthma treatment for several centuries in many Asian countries, and have been shown to effectively relieve symptoms.We found that the inhibition of bronchoconstriction by the CTCM was attenuated by pretreatment with propranolol, suggesting that the CTCM has a bronchodilator effect that is associated with beta-adrenergic receptor.In conclusion, our results suggested that the CTCM is a kind of bronchodilator and also a mast cell stabilizer.

View Article: PubMed Central - HTML - PubMed

ABSTRACT

Background: Traditional Chinese medicines have been used for anti-asthma treatment for several centuries in many Asian countries, and have been shown to effectively relieve symptoms. Our previous study demonstrated that a complex traditional Chinese medicine (CTCM) administered in nebulized form through the intratracheal route is effective against early-phase air-flow obstruction and can inhibit IL-5 production in ovalbumin (OVA)-sensitized guinea pigs. However, the antiasthmatic mechanisms of CTCMs are still unclear.

Methods: In this study, we examined the underlying mechanism of a CTCM that we used in our previous study in order to ascertain its function in the early-phase response to OVA challenge.

Results: We found that the inhibition of bronchoconstriction by the CTCM was attenuated by pretreatment with propranolol, suggesting that the CTCM has a bronchodilator effect that is associated with beta-adrenergic receptor. Our results also showed that the CTCM inhibited histamine and IL-4 secretion in the OVA-induced airway hypersensitivity in guinea pigs at 5 min post-OVA challenge, and in vitro study revealed that the CTCM is able to stabilize mast cells.

Conclusion: In conclusion, our results suggested that the CTCM is a kind of bronchodilator and also a mast cell stabilizer. Our findings provide useful information regarding the possible mechanism of the CTCM, and show its potential for application in the treatment of allergenic airway disease.

Show MeSH
Related in: MedlinePlus