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Comparative secretome analysis of four isogenic Bacillus clausii probiotic strains.

Lippolis R, Siciliano RA, Mazzeo MF, Abbrescia A, Gnoni A, Sardanelli AM, Papa S - Proteome Sci (2013)

Bottom Line: Among these, some proteins with documented activity in the interaction with host cells were identified, such as the glycolytic enzyme enolase, with a putative plasminogen-binding activity, GroEL, a molecular chaperone shown to be able to bind to mucin, and flagellin protein, a structural flagella protein and a putative immunomodulation agent.This study shows, for the first time, differences in the secretome of the OC, SIN, NR and T B. clausii strains.These differences indicate that specific secretome features characterize each of the four strains despite their genotypic similarity.

View Article: PubMed Central - HTML - PubMed

Affiliation: Institute of Biomembranes and Bioenergetics, Italian National Research Council (CNR), Via Amendola 165/A, Bari, Italy. r.lippolis@ibbe.cnr.it.

ABSTRACT

Background: The spore-bearing alkaliphilic Bacillus species constitute a large, heterogeneous group of microorganisms, important for their ability to produce enzymes, antibodies and metabolites of potential medical use. Some Bacillus species are currently being used for manufacturing probiotic products consisting of bacterial spores, exhibiting specific features (colonization, immune-stimulation and antimicrobial activity) that can account for their claimed probiotic properties. In the present work a comparative proteomic study was performed aimed at characterizing the secretome of four closely related isogenic O/C, SIN, N/R and T B. clausii strains, already marketed in a pharmaceutical mixture as probiotics.

Results: Proteomic analyses revealed a high degree of concordance among the four secretomes, although some proteins exhibited considerable variations in their expression level in the four strains. Among these, some proteins with documented activity in the interaction with host cells were identified, such as the glycolytic enzyme enolase, with a putative plasminogen-binding activity, GroEL, a molecular chaperone shown to be able to bind to mucin, and flagellin protein, a structural flagella protein and a putative immunomodulation agent.

Conclusion: This study shows, for the first time, differences in the secretome of the OC, SIN, NR and T B. clausii strains. These differences indicate that specific secretome features characterize each of the four strains despite their genotypic similarity. This could confer to the B. clausii strains specific probiotic functions associated with the differentially expressed proteins and indicate that they can cooperate as probiotics as the secretome components of each strain could contribute to the overall activity of a mixed probiotic preparation.

No MeSH data available.


Related in: MedlinePlus

Expression profile in the four strains of other identified proteins. Each spot is indicated with a match ID. The error bars (SEM) are indicated.
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Figure 6: Expression profile in the four strains of other identified proteins. Each spot is indicated with a match ID. The error bars (SEM) are indicated.

Mentions: These results are shown in Figures 5, 6 and summarized in Table 1 and Additional file 6: Table S2.


Comparative secretome analysis of four isogenic Bacillus clausii probiotic strains.

Lippolis R, Siciliano RA, Mazzeo MF, Abbrescia A, Gnoni A, Sardanelli AM, Papa S - Proteome Sci (2013)

Expression profile in the four strains of other identified proteins. Each spot is indicated with a match ID. The error bars (SEM) are indicated.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3716886&req=5

Figure 6: Expression profile in the four strains of other identified proteins. Each spot is indicated with a match ID. The error bars (SEM) are indicated.
Mentions: These results are shown in Figures 5, 6 and summarized in Table 1 and Additional file 6: Table S2.

Bottom Line: Among these, some proteins with documented activity in the interaction with host cells were identified, such as the glycolytic enzyme enolase, with a putative plasminogen-binding activity, GroEL, a molecular chaperone shown to be able to bind to mucin, and flagellin protein, a structural flagella protein and a putative immunomodulation agent.This study shows, for the first time, differences in the secretome of the OC, SIN, NR and T B. clausii strains.These differences indicate that specific secretome features characterize each of the four strains despite their genotypic similarity.

View Article: PubMed Central - HTML - PubMed

Affiliation: Institute of Biomembranes and Bioenergetics, Italian National Research Council (CNR), Via Amendola 165/A, Bari, Italy. r.lippolis@ibbe.cnr.it.

ABSTRACT

Background: The spore-bearing alkaliphilic Bacillus species constitute a large, heterogeneous group of microorganisms, important for their ability to produce enzymes, antibodies and metabolites of potential medical use. Some Bacillus species are currently being used for manufacturing probiotic products consisting of bacterial spores, exhibiting specific features (colonization, immune-stimulation and antimicrobial activity) that can account for their claimed probiotic properties. In the present work a comparative proteomic study was performed aimed at characterizing the secretome of four closely related isogenic O/C, SIN, N/R and T B. clausii strains, already marketed in a pharmaceutical mixture as probiotics.

Results: Proteomic analyses revealed a high degree of concordance among the four secretomes, although some proteins exhibited considerable variations in their expression level in the four strains. Among these, some proteins with documented activity in the interaction with host cells were identified, such as the glycolytic enzyme enolase, with a putative plasminogen-binding activity, GroEL, a molecular chaperone shown to be able to bind to mucin, and flagellin protein, a structural flagella protein and a putative immunomodulation agent.

Conclusion: This study shows, for the first time, differences in the secretome of the OC, SIN, NR and T B. clausii strains. These differences indicate that specific secretome features characterize each of the four strains despite their genotypic similarity. This could confer to the B. clausii strains specific probiotic functions associated with the differentially expressed proteins and indicate that they can cooperate as probiotics as the secretome components of each strain could contribute to the overall activity of a mixed probiotic preparation.

No MeSH data available.


Related in: MedlinePlus