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Lr67 and Lr34 rust resistance genes have much in common--they confer broad spectrum resistance to multiple pathogens in wheat.

Spielmeyer W, Mago R, Wellings C, Ayliffe M - BMC Plant Biol. (2013)

Bottom Line: Detailed study of Lr34 mutants revealed that subtle changes in resistance response to multiple pathogens were correlated with mutational changes in the predicted protein.Recovery of independent Lr67 mutants indicates that as for Lr34, a single gene at the Lr67 locus is likely to confer resistance to multiple pathogens.The infection phenotypes of Lr67 mutants closely resembled that of Lr34 mutants.

View Article: PubMed Central - HTML - PubMed

Affiliation: CSIRO Plant Industry, GPO Box 1600, Canberra 2601, ACT, Australia. wolfgang.spielmeyer@csiro.au

ABSTRACT

Background: Adult plant rust resistance genes Lr67 and Lr34 confer race non-specific resistance to multiple fungal pathogens of wheat. Induced, susceptible mutants were characterised for both genes.

Results: Three categories of Lr34 mutants were identified that were either partial susceptible, fully susceptible or hyper-susceptible to stripe rust and leaf rust. The likely impact of the mutational change on the predicted Lr34 protein correlated with differences in response to rust infection. Four independent Lr67 mutants were recovered that were susceptible to stripe rust, leaf rust and stem rust pathogens, including one possible hyper-susceptible Lr67 mutant.

Conclusions: Detailed study of Lr34 mutants revealed that subtle changes in resistance response to multiple pathogens were correlated with mutational changes in the predicted protein. Recovery of independent Lr67 mutants indicates that as for Lr34, a single gene at the Lr67 locus is likely to confer resistance to multiple pathogens. The infection phenotypes of Lr67 mutants closely resembled that of Lr34 mutants.

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Stem rust infection on the peduncle of field grown PI 250413 (donor Lr67), Thatcher (Tc), RL6077 (Lr67) and derived mutants M1723, M2255 and M2257. M2255 was more susceptible to stem rust than Thatcher.
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Figure 6: Stem rust infection on the peduncle of field grown PI 250413 (donor Lr67), Thatcher (Tc), RL6077 (Lr67) and derived mutants M1723, M2255 and M2257. M2255 was more susceptible to stem rust than Thatcher.

Mentions: The effect of Lr67 on resistance to stem rust was also recorded. RL6077 (5MR-10MR) produced fewer pustules under field conditions than the moderately resistant/susceptible Thatcher line (30–40 MRMS) (Figure 6). Mutants M1723, M2006 and M2257 were moderately resistant/susceptible and similar to Thatcher, but M2255, which was more susceptible to stripe rust, was also more susceptible to stem rust (60MS) than the other mutants (Figure 6). The Lr67 donor line PI 250413 was fully susceptible to stem rust (90S) indicating that Lr67 alone was insufficient to confer stem rust resistance and that the stem rust resistance present in the Thatcher backcross derivative, RL6077, was due to interaction of Lr67 with other gene(s) present in this genotype. Lr67 mutants were also susceptible to leaf rust although the field infection was more variable than for stripe rust and stem rust. Several attempts to induce a resistance response to leaf rust in seedlings when grown at low temperature failed in RL6077. In contrast to RL6058, RL6077 was fully susceptible to leaf rust under these conditions. Leaf tip necrosis which was associated with Lr67 was also lacking in these mutant lines.


Lr67 and Lr34 rust resistance genes have much in common--they confer broad spectrum resistance to multiple pathogens in wheat.

Spielmeyer W, Mago R, Wellings C, Ayliffe M - BMC Plant Biol. (2013)

Stem rust infection on the peduncle of field grown PI 250413 (donor Lr67), Thatcher (Tc), RL6077 (Lr67) and derived mutants M1723, M2255 and M2257. M2255 was more susceptible to stem rust than Thatcher.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3716802&req=5

Figure 6: Stem rust infection on the peduncle of field grown PI 250413 (donor Lr67), Thatcher (Tc), RL6077 (Lr67) and derived mutants M1723, M2255 and M2257. M2255 was more susceptible to stem rust than Thatcher.
Mentions: The effect of Lr67 on resistance to stem rust was also recorded. RL6077 (5MR-10MR) produced fewer pustules under field conditions than the moderately resistant/susceptible Thatcher line (30–40 MRMS) (Figure 6). Mutants M1723, M2006 and M2257 were moderately resistant/susceptible and similar to Thatcher, but M2255, which was more susceptible to stripe rust, was also more susceptible to stem rust (60MS) than the other mutants (Figure 6). The Lr67 donor line PI 250413 was fully susceptible to stem rust (90S) indicating that Lr67 alone was insufficient to confer stem rust resistance and that the stem rust resistance present in the Thatcher backcross derivative, RL6077, was due to interaction of Lr67 with other gene(s) present in this genotype. Lr67 mutants were also susceptible to leaf rust although the field infection was more variable than for stripe rust and stem rust. Several attempts to induce a resistance response to leaf rust in seedlings when grown at low temperature failed in RL6077. In contrast to RL6058, RL6077 was fully susceptible to leaf rust under these conditions. Leaf tip necrosis which was associated with Lr67 was also lacking in these mutant lines.

Bottom Line: Detailed study of Lr34 mutants revealed that subtle changes in resistance response to multiple pathogens were correlated with mutational changes in the predicted protein.Recovery of independent Lr67 mutants indicates that as for Lr34, a single gene at the Lr67 locus is likely to confer resistance to multiple pathogens.The infection phenotypes of Lr67 mutants closely resembled that of Lr34 mutants.

View Article: PubMed Central - HTML - PubMed

Affiliation: CSIRO Plant Industry, GPO Box 1600, Canberra 2601, ACT, Australia. wolfgang.spielmeyer@csiro.au

ABSTRACT

Background: Adult plant rust resistance genes Lr67 and Lr34 confer race non-specific resistance to multiple fungal pathogens of wheat. Induced, susceptible mutants were characterised for both genes.

Results: Three categories of Lr34 mutants were identified that were either partial susceptible, fully susceptible or hyper-susceptible to stripe rust and leaf rust. The likely impact of the mutational change on the predicted Lr34 protein correlated with differences in response to rust infection. Four independent Lr67 mutants were recovered that were susceptible to stripe rust, leaf rust and stem rust pathogens, including one possible hyper-susceptible Lr67 mutant.

Conclusions: Detailed study of Lr34 mutants revealed that subtle changes in resistance response to multiple pathogens were correlated with mutational changes in the predicted protein. Recovery of independent Lr67 mutants indicates that as for Lr34, a single gene at the Lr67 locus is likely to confer resistance to multiple pathogens. The infection phenotypes of Lr67 mutants closely resembled that of Lr34 mutants.

Show MeSH
Related in: MedlinePlus