Limits...
MiR-214 regulate gastric cancer cell proliferation, migration and invasion by targeting PTEN.

Yang TS, Yang XH, Wang XD, Wang YL, Zhou B, Song ZS - Cancer Cell Int. (2013)

Bottom Line: MicroRNAs are a class of small non-coding RNAs that play an important role in various human tumor initiation and progression by regulating gene expression negatively.The expression level of miR-214 is significantly associated with clinical progression and poor prognosis according to the analysis of the clinicopathologic data.We also found that the miR-214 levels are inversely correlated with PTEN in tumor tissues.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Hepato-Biliary-Pancreatic Surgery, Tenth Peoples' Hospital, School of Medicine, Tongji University, 301 Middle Yanchang Road, Shanghai 200072, China. songzhenshun@yahoo.com.cn.

ABSTRACT

Background: MicroRNAs are a class of small non-coding RNAs that play an important role in various human tumor initiation and progression by regulating gene expression negatively. The aim of this study was to investigate the effect of miR-214 on cell proliferation, migration and invasion, as well as the functional connection between miR-214 and PTEN in gastric cancer.

Methods: miR-214 and PTEN expression was determined in gastric cancer and matched normal tissues, and human gastric cancer cell lines by quantitative real-time PCR. The roles of miR-214 in cell proliferation, migration and invasion were analyzed with anti-miR-214 transfected cells. In addition, the regulation of PTEN by miR-214 was evaluated by Western blotting and luciferase reporter assays.

Results: miR-214 was noted to be highly overexpressed in gastric cancer tissues and cell lines using qRT-PCR. The expression level of miR-214 is significantly associated with clinical progression and poor prognosis according to the analysis of the clinicopathologic data. We also found that the miR-214 levels are inversely correlated with PTEN in tumor tissues. And PTEN expression level is also associated with metastasis and invasion of gastric cancer. In addition, knockdown of miR-214 could significantly inhibit proliferation, migration and invasion of gastric cancer cells. Moreover, we demonstrate that PTEN is regulated negatively by miR-214 through a miR-214 binding site within the 3'-UTR of PTEN at the posttranscriptional level in gastric cancer cells.

Conclusions: These findings indicated that miR-214 regulated the proliferation, migration and invasion by targeting PTEN post-transcriptionally in gastric cancer. It may be a novel potential therapeutic agent for gastric cancer.

No MeSH data available.


Related in: MedlinePlus

Expression of miR-214 and PTEN in gastric cancer cell and tissue specimens. Detection of miR-214 and PTEN mRNA by qRT-PCR using U6 snRNA for normalization. Both SGC-7901 and BGC-823 cells express higher levels of miR-214 compared with normal gastric mucosa epithelial cell lines GES-1 (A), the expression of miR-214 in gastric cancer tissues is higher than that in normal gastric mucosa tissues (B). Whereas, the expression of PTEN mRNA in gastric cancer tissues is lower than that in normal gastric mucosa tissues (C), in addition, we found an inverse correlation between the expression of miR-214 and the level of PTEN mRNA in gastric cancer tissues, but not in normal tissues (D, E). Kaplan-Meier survival curves for 120 gastric cancer cases, low expression of miR-214 (red) was defined as long survival, and high expression (green) was defined as short survival (F). *P < 0.05; **P < 0.01.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC3716801&req=5

Figure 1: Expression of miR-214 and PTEN in gastric cancer cell and tissue specimens. Detection of miR-214 and PTEN mRNA by qRT-PCR using U6 snRNA for normalization. Both SGC-7901 and BGC-823 cells express higher levels of miR-214 compared with normal gastric mucosa epithelial cell lines GES-1 (A), the expression of miR-214 in gastric cancer tissues is higher than that in normal gastric mucosa tissues (B). Whereas, the expression of PTEN mRNA in gastric cancer tissues is lower than that in normal gastric mucosa tissues (C), in addition, we found an inverse correlation between the expression of miR-214 and the level of PTEN mRNA in gastric cancer tissues, but not in normal tissues (D, E). Kaplan-Meier survival curves for 120 gastric cancer cases, low expression of miR-214 (red) was defined as long survival, and high expression (green) was defined as short survival (F). *P < 0.05; **P < 0.01.

Mentions: The quantitative RT-PCR detection results showed that the expression levels of miR-214 were significantly higher in gastric cancer cell lines in comparison with the normal gastric mucosa epithelial cell lines (Figure 1A). In the meantime, miR-214 overexpression is also observed in gastric cancer tissues compared to normal gastric mucosa tissues (Figure 1B). Furthermore, the expression of miR-214 is significantly associated with invasion, metastasis and TNM stage according to the clinicopathologic data from the gastric cancer patients (Table 1), and clinical relevance was confirmed by the observation that miR-214 overexpression correlated with poor prognosis (Figure 1F). All these evidence indicated that miR-214 may be involved in gastric cancer carcinogenesis.


MiR-214 regulate gastric cancer cell proliferation, migration and invasion by targeting PTEN.

Yang TS, Yang XH, Wang XD, Wang YL, Zhou B, Song ZS - Cancer Cell Int. (2013)

Expression of miR-214 and PTEN in gastric cancer cell and tissue specimens. Detection of miR-214 and PTEN mRNA by qRT-PCR using U6 snRNA for normalization. Both SGC-7901 and BGC-823 cells express higher levels of miR-214 compared with normal gastric mucosa epithelial cell lines GES-1 (A), the expression of miR-214 in gastric cancer tissues is higher than that in normal gastric mucosa tissues (B). Whereas, the expression of PTEN mRNA in gastric cancer tissues is lower than that in normal gastric mucosa tissues (C), in addition, we found an inverse correlation between the expression of miR-214 and the level of PTEN mRNA in gastric cancer tissues, but not in normal tissues (D, E). Kaplan-Meier survival curves for 120 gastric cancer cases, low expression of miR-214 (red) was defined as long survival, and high expression (green) was defined as short survival (F). *P < 0.05; **P < 0.01.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3716801&req=5

Figure 1: Expression of miR-214 and PTEN in gastric cancer cell and tissue specimens. Detection of miR-214 and PTEN mRNA by qRT-PCR using U6 snRNA for normalization. Both SGC-7901 and BGC-823 cells express higher levels of miR-214 compared with normal gastric mucosa epithelial cell lines GES-1 (A), the expression of miR-214 in gastric cancer tissues is higher than that in normal gastric mucosa tissues (B). Whereas, the expression of PTEN mRNA in gastric cancer tissues is lower than that in normal gastric mucosa tissues (C), in addition, we found an inverse correlation between the expression of miR-214 and the level of PTEN mRNA in gastric cancer tissues, but not in normal tissues (D, E). Kaplan-Meier survival curves for 120 gastric cancer cases, low expression of miR-214 (red) was defined as long survival, and high expression (green) was defined as short survival (F). *P < 0.05; **P < 0.01.
Mentions: The quantitative RT-PCR detection results showed that the expression levels of miR-214 were significantly higher in gastric cancer cell lines in comparison with the normal gastric mucosa epithelial cell lines (Figure 1A). In the meantime, miR-214 overexpression is also observed in gastric cancer tissues compared to normal gastric mucosa tissues (Figure 1B). Furthermore, the expression of miR-214 is significantly associated with invasion, metastasis and TNM stage according to the clinicopathologic data from the gastric cancer patients (Table 1), and clinical relevance was confirmed by the observation that miR-214 overexpression correlated with poor prognosis (Figure 1F). All these evidence indicated that miR-214 may be involved in gastric cancer carcinogenesis.

Bottom Line: MicroRNAs are a class of small non-coding RNAs that play an important role in various human tumor initiation and progression by regulating gene expression negatively.The expression level of miR-214 is significantly associated with clinical progression and poor prognosis according to the analysis of the clinicopathologic data.We also found that the miR-214 levels are inversely correlated with PTEN in tumor tissues.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Hepato-Biliary-Pancreatic Surgery, Tenth Peoples' Hospital, School of Medicine, Tongji University, 301 Middle Yanchang Road, Shanghai 200072, China. songzhenshun@yahoo.com.cn.

ABSTRACT

Background: MicroRNAs are a class of small non-coding RNAs that play an important role in various human tumor initiation and progression by regulating gene expression negatively. The aim of this study was to investigate the effect of miR-214 on cell proliferation, migration and invasion, as well as the functional connection between miR-214 and PTEN in gastric cancer.

Methods: miR-214 and PTEN expression was determined in gastric cancer and matched normal tissues, and human gastric cancer cell lines by quantitative real-time PCR. The roles of miR-214 in cell proliferation, migration and invasion were analyzed with anti-miR-214 transfected cells. In addition, the regulation of PTEN by miR-214 was evaluated by Western blotting and luciferase reporter assays.

Results: miR-214 was noted to be highly overexpressed in gastric cancer tissues and cell lines using qRT-PCR. The expression level of miR-214 is significantly associated with clinical progression and poor prognosis according to the analysis of the clinicopathologic data. We also found that the miR-214 levels are inversely correlated with PTEN in tumor tissues. And PTEN expression level is also associated with metastasis and invasion of gastric cancer. In addition, knockdown of miR-214 could significantly inhibit proliferation, migration and invasion of gastric cancer cells. Moreover, we demonstrate that PTEN is regulated negatively by miR-214 through a miR-214 binding site within the 3'-UTR of PTEN at the posttranscriptional level in gastric cancer cells.

Conclusions: These findings indicated that miR-214 regulated the proliferation, migration and invasion by targeting PTEN post-transcriptionally in gastric cancer. It may be a novel potential therapeutic agent for gastric cancer.

No MeSH data available.


Related in: MedlinePlus