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Bartonella species in raccoons and feral cats, Georgia, USA.

Hwang J, Gottdenker NL - Emerging Infect. Dis. (2013)

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This study describes 2 Bartonella species in an urban population of raccoons and compares these findings to Bartonella infection in sympatric feral cats (Felis catus)... Extracted DNA was used to amplify the 16S–23S rRNA intergenic spacer region of Bartonella spp. by nested PCR... For outer PCR, we used primers QHVE-1 (5′-TTCAGATGATGATCCCAAGC-3′) and QHVE-3 (5′-AACATGTCTGAATATATCTTC-3′)... B. koehlerae was amplified from 1 feral cat sample and 1 raccoon sample (99% sequence homology with a B. koehlerae sequence, GenBank accession no... AF312490)... Two feral cat samples were identified as containing B. clarridgeiae and showed 98% and 100% sequence homology with a B. clarridgeiae sequence (GenBank accession no... AF167989) (Table; Figure, Appendix)... Our results are useful because raccoons are potential reservoir hosts of zoonotic B. henselae and B. koehlerae, in addition to B. rochalimae, and there could be cross-species transmission of Bartonella spp. between feral cats and raccoons... In this study, a relatively high proportion of raccoons were infected with B. henselae, implying that there is spillover of B. henselae from feral cats to raccoons or that raccoons are another active reservoir for B. henselae... B. clarridgeiae and B. koehlerae are also zoonotic; cats are primary reservoirs, and humans and dogs are accidental hosts... However, B. clarridgeiae was recently detected in rodent fleas in China and B. koehlerae was isolated from feral pigs from the southeastern United States, suggesting that these pathogens also have multiple reservoir species... Clarifying whether Bartonella infections in raccoons are caused by spillover from feral cats needs further study... Additional samples from raccoons and other species in urbanized and undeveloped habitats with different host species composition (e.g., cat-free environment) might enable further Bartonella spp. characterization in wildlife... We suspect urban raccoons and feral cats play a major role in Bartonella spp. transmission.

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Phylogenetic tree of intergenic spacer region genes of Bartonella species inferred by the neighbor-joining method using the maximum composite likelihood method. Samples from this study are indicated by a solid triangle. GenBank accession numbers are indicated after species name.
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Figure 1: Phylogenetic tree of intergenic spacer region genes of Bartonella species inferred by the neighbor-joining method using the maximum composite likelihood method. Samples from this study are indicated by a solid triangle. GenBank accession numbers are indicated after species name.

Mentions: Of 74 samples analyzed (37 raccoon, 37 feral cat), 16 (43%) raccoon samples and 18 (48%) feral cat samples were positive for Bartonella spp. by PCR. Thirteen positive raccoon samples and 16 positive feral cat samples were sequenced. Twelve positive raccoon samples and 13 positive feral cat samples contained Bartonella henselae. B. koehlerae was amplified from 1 feral cat sample and 1 raccoon sample (99% sequence homology with a B. koehlerae sequence, GenBank accession no. AF312490). Two feral cat samples were identified as containing B. clarridgeiae and showed 98% and 100% sequence homology with a B. clarridgeiae sequence (GenBank accession no. AF167989) (Table; Figure, Appendix).


Bartonella species in raccoons and feral cats, Georgia, USA.

Hwang J, Gottdenker NL - Emerging Infect. Dis. (2013)

Phylogenetic tree of intergenic spacer region genes of Bartonella species inferred by the neighbor-joining method using the maximum composite likelihood method. Samples from this study are indicated by a solid triangle. GenBank accession numbers are indicated after species name.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3713986&req=5

Figure 1: Phylogenetic tree of intergenic spacer region genes of Bartonella species inferred by the neighbor-joining method using the maximum composite likelihood method. Samples from this study are indicated by a solid triangle. GenBank accession numbers are indicated after species name.
Mentions: Of 74 samples analyzed (37 raccoon, 37 feral cat), 16 (43%) raccoon samples and 18 (48%) feral cat samples were positive for Bartonella spp. by PCR. Thirteen positive raccoon samples and 16 positive feral cat samples were sequenced. Twelve positive raccoon samples and 13 positive feral cat samples contained Bartonella henselae. B. koehlerae was amplified from 1 feral cat sample and 1 raccoon sample (99% sequence homology with a B. koehlerae sequence, GenBank accession no. AF312490). Two feral cat samples were identified as containing B. clarridgeiae and showed 98% and 100% sequence homology with a B. clarridgeiae sequence (GenBank accession no. AF167989) (Table; Figure, Appendix).

View Article: PubMed Central - PubMed

AUTOMATICALLY GENERATED EXCERPT
Please rate it.

This study describes 2 Bartonella species in an urban population of raccoons and compares these findings to Bartonella infection in sympatric feral cats (Felis catus)... Extracted DNA was used to amplify the 16S–23S rRNA intergenic spacer region of Bartonella spp. by nested PCR... For outer PCR, we used primers QHVE-1 (5′-TTCAGATGATGATCCCAAGC-3′) and QHVE-3 (5′-AACATGTCTGAATATATCTTC-3′)... B. koehlerae was amplified from 1 feral cat sample and 1 raccoon sample (99% sequence homology with a B. koehlerae sequence, GenBank accession no... AF312490)... Two feral cat samples were identified as containing B. clarridgeiae and showed 98% and 100% sequence homology with a B. clarridgeiae sequence (GenBank accession no... AF167989) (Table; Figure, Appendix)... Our results are useful because raccoons are potential reservoir hosts of zoonotic B. henselae and B. koehlerae, in addition to B. rochalimae, and there could be cross-species transmission of Bartonella spp. between feral cats and raccoons... In this study, a relatively high proportion of raccoons were infected with B. henselae, implying that there is spillover of B. henselae from feral cats to raccoons or that raccoons are another active reservoir for B. henselae... B. clarridgeiae and B. koehlerae are also zoonotic; cats are primary reservoirs, and humans and dogs are accidental hosts... However, B. clarridgeiae was recently detected in rodent fleas in China and B. koehlerae was isolated from feral pigs from the southeastern United States, suggesting that these pathogens also have multiple reservoir species... Clarifying whether Bartonella infections in raccoons are caused by spillover from feral cats needs further study... Additional samples from raccoons and other species in urbanized and undeveloped habitats with different host species composition (e.g., cat-free environment) might enable further Bartonella spp. characterization in wildlife... We suspect urban raccoons and feral cats play a major role in Bartonella spp. transmission.

Show MeSH
Related in: MedlinePlus