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Schmallenberg virus among female lambs, Belgium, 2012.

Claine F, Coupeau D, Wiggers L, Muylkens B, Kirschvink N - Emerging Infect. Dis. (2013)

Bottom Line: Reemergence of Schmallenberg virus (SBV) occurred among lambs (n = 50) in a sheep flock in Belgium between mid-July and mid-October 2012.Bimonthly assessment by quantitative reverse transcription PCR and seroneutralization demonstrated that 100% of lambs were infected.Viremia duration may be longer in naturally infected than in experimentally infected animals.

View Article: PubMed Central - PubMed

Affiliation: Namur Research Institute for Life Sciences, University of Namur, Namur, Belgium.

ABSTRACT
Reemergence of Schmallenberg virus (SBV) occurred among lambs (n = 50) in a sheep flock in Belgium between mid-July and mid-October 2012. Bimonthly assessment by quantitative reverse transcription PCR and seroneutralization demonstrated that 100% of lambs were infected. Viremia duration may be longer in naturally infected than in experimentally infected animals.

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Related in: MedlinePlus

Time course of Schmallenberg virus spread among 50 infection-naive female lambs assessed bimonthly by real-time quantitative reverse transcription PCR (RT-qPCR) and seroneutralization (SN). Cumulative positive results (cycle threshold <40 and log2 50% effective dose >3.5) obtained during July–October 2012 are expressed as percentages.
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Figure 1: Time course of Schmallenberg virus spread among 50 infection-naive female lambs assessed bimonthly by real-time quantitative reverse transcription PCR (RT-qPCR) and seroneutralization (SN). Cumulative positive results (cycle threshold <40 and log2 50% effective dose >3.5) obtained during July–October 2012 are expressed as percentages.

Mentions: As shown in Figure 1, the SBV infection of the sentinels evidenced by RT-qPCR was first detected around mid-July and ended in mid-October 2012. Before this period, RT-qPCR results were negative and decrease of colostrum derived antibodies was observed (data not shown). Few animals were infected in July (3 animals were positive by using RT-qPCR on July 27) and at the beginning of August. Of the positive RT-qPCR results, 80% were found between mid-August and late September. By October 17, >1 positive RT-qPCR result had been detected for each animal (median Ct 37.03; minimum 29.4, maximum 39.5). SN assay revealed positive results for SBV antibody titers within 2–4 weeks after infection evidenced by RT-qPCR results. These findings are similar to observations made after experimental and natural SBV infection in cattle (1,10). During the period of monitoring, no clinical signs were detected.


Schmallenberg virus among female lambs, Belgium, 2012.

Claine F, Coupeau D, Wiggers L, Muylkens B, Kirschvink N - Emerging Infect. Dis. (2013)

Time course of Schmallenberg virus spread among 50 infection-naive female lambs assessed bimonthly by real-time quantitative reverse transcription PCR (RT-qPCR) and seroneutralization (SN). Cumulative positive results (cycle threshold <40 and log2 50% effective dose >3.5) obtained during July–October 2012 are expressed as percentages.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3713981&req=5

Figure 1: Time course of Schmallenberg virus spread among 50 infection-naive female lambs assessed bimonthly by real-time quantitative reverse transcription PCR (RT-qPCR) and seroneutralization (SN). Cumulative positive results (cycle threshold <40 and log2 50% effective dose >3.5) obtained during July–October 2012 are expressed as percentages.
Mentions: As shown in Figure 1, the SBV infection of the sentinels evidenced by RT-qPCR was first detected around mid-July and ended in mid-October 2012. Before this period, RT-qPCR results were negative and decrease of colostrum derived antibodies was observed (data not shown). Few animals were infected in July (3 animals were positive by using RT-qPCR on July 27) and at the beginning of August. Of the positive RT-qPCR results, 80% were found between mid-August and late September. By October 17, >1 positive RT-qPCR result had been detected for each animal (median Ct 37.03; minimum 29.4, maximum 39.5). SN assay revealed positive results for SBV antibody titers within 2–4 weeks after infection evidenced by RT-qPCR results. These findings are similar to observations made after experimental and natural SBV infection in cattle (1,10). During the period of monitoring, no clinical signs were detected.

Bottom Line: Reemergence of Schmallenberg virus (SBV) occurred among lambs (n = 50) in a sheep flock in Belgium between mid-July and mid-October 2012.Bimonthly assessment by quantitative reverse transcription PCR and seroneutralization demonstrated that 100% of lambs were infected.Viremia duration may be longer in naturally infected than in experimentally infected animals.

View Article: PubMed Central - PubMed

Affiliation: Namur Research Institute for Life Sciences, University of Namur, Namur, Belgium.

ABSTRACT
Reemergence of Schmallenberg virus (SBV) occurred among lambs (n = 50) in a sheep flock in Belgium between mid-July and mid-October 2012. Bimonthly assessment by quantitative reverse transcription PCR and seroneutralization demonstrated that 100% of lambs were infected. Viremia duration may be longer in naturally infected than in experimentally infected animals.

Show MeSH
Related in: MedlinePlus