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Transmission of Streptococcus equi subspecies zooepidemicus infection from horses to humans.

Pelkonen S, Lindahl SB, Suomala P, Karhukorpi J, Vuorinen S, Koivula I, Väisänen T, Pentikäinen J, Autio T, Tuuminen T - Emerging Infect. Dis. (2013)

Bottom Line: The isolates were analyzed by using pulsed-field gel electrophoresis, multilocus sequence typing, and sequencing of the szP gene.Molecular typing methods showed that human and equine isolates were identical or closely related.As leisure and professional equine sports continue to grow, this infection should be recognized as an emerging zoonosis.

View Article: PubMed Central - PubMed

Affiliation: Finnish Food Safety Authority Evira, Kuopio,Finland. sinikka.pelkonen@evira.fi

ABSTRACT
Streptococcus equi subspecies zooepidemicus (S. zooepidemicus) is a zoonotic pathogen for persons in contact with horses. In horses, S. zooepidemicus is an opportunistic pathogen, but human infections associated with S. zooepidemicus are often severe. Within 6 months in 2011, 3 unrelated cases of severe, disseminated S. zooepidemicus infection occurred in men working with horses in eastern Finland. To clarify the pathogen's epidemiology, we describe the clinical features of the infection in 3 patients and compare the S. zooepidemicus isolates from the human cases with S. zooepidemicus isolates from horses. The isolates were analyzed by using pulsed-field gel electrophoresis, multilocus sequence typing, and sequencing of the szP gene. Molecular typing methods showed that human and equine isolates were identical or closely related. These results emphasize that S. zooepidemicus transmitted from horses can lead to severe infections in humans. As leisure and professional equine sports continue to grow, this infection should be recognized as an emerging zoonosis.

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Pulsed-field gel electrophoresis of Streptococcus equi subspecies zooepidemicus isolates using SmaI. The lanes are marked with the number of the respective isolate. DNA of Salmonella enterica serovar Braenderup H9182 was used as a molecular marker.
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Figure 2: Pulsed-field gel electrophoresis of Streptococcus equi subspecies zooepidemicus isolates using SmaI. The lanes are marked with the number of the respective isolate. DNA of Salmonella enterica serovar Braenderup H9182 was used as a molecular marker.

Mentions: The human isolates Hum1 (patient 1) and Hum2 (patient 2) displayed an szP sequence (GenBank accession no. AF519489) and MLST sequence type (ST-10) identical to the equine isolate 648/11 (stable A) (Table 2). Hum1 was also identical to equine isolate 648/11 on PFGE (Figure 2). Hum2, however, differed from Hum1 and 648/11 by 6 bands on the PFGE profile. The third human isolate, Hum3 (patient 3), was closely related to 1 equine isolate (6939/10) from an unrelated stable (stable D). These isolates displayed an identical szP sequence (accession no. AF519488). Their PFGE profiles were almost identical, and the MLST types ST-209 (Hum3) and ST-201 (6939/10) were DLVs. None of the other equine isolates displayed the same szP sequence type or MLST STs as the human isolates. Among the 5 S. zooepidemicus isolates from stable A, 645/11 was identical to 647/11 on the basis of the MLST ST (ST-175), szP type (II), and PFGE profile. All other isolates differed from each other. Several equine isolates displayed szP sequences not previously described in GenBank (645/11, 646/11, 647/11, and 1128). All szP sequencing results and corresponding GenBank accession numbers are listed in Table 2.


Transmission of Streptococcus equi subspecies zooepidemicus infection from horses to humans.

Pelkonen S, Lindahl SB, Suomala P, Karhukorpi J, Vuorinen S, Koivula I, Väisänen T, Pentikäinen J, Autio T, Tuuminen T - Emerging Infect. Dis. (2013)

Pulsed-field gel electrophoresis of Streptococcus equi subspecies zooepidemicus isolates using SmaI. The lanes are marked with the number of the respective isolate. DNA of Salmonella enterica serovar Braenderup H9182 was used as a molecular marker.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3713971&req=5

Figure 2: Pulsed-field gel electrophoresis of Streptococcus equi subspecies zooepidemicus isolates using SmaI. The lanes are marked with the number of the respective isolate. DNA of Salmonella enterica serovar Braenderup H9182 was used as a molecular marker.
Mentions: The human isolates Hum1 (patient 1) and Hum2 (patient 2) displayed an szP sequence (GenBank accession no. AF519489) and MLST sequence type (ST-10) identical to the equine isolate 648/11 (stable A) (Table 2). Hum1 was also identical to equine isolate 648/11 on PFGE (Figure 2). Hum2, however, differed from Hum1 and 648/11 by 6 bands on the PFGE profile. The third human isolate, Hum3 (patient 3), was closely related to 1 equine isolate (6939/10) from an unrelated stable (stable D). These isolates displayed an identical szP sequence (accession no. AF519488). Their PFGE profiles were almost identical, and the MLST types ST-209 (Hum3) and ST-201 (6939/10) were DLVs. None of the other equine isolates displayed the same szP sequence type or MLST STs as the human isolates. Among the 5 S. zooepidemicus isolates from stable A, 645/11 was identical to 647/11 on the basis of the MLST ST (ST-175), szP type (II), and PFGE profile. All other isolates differed from each other. Several equine isolates displayed szP sequences not previously described in GenBank (645/11, 646/11, 647/11, and 1128). All szP sequencing results and corresponding GenBank accession numbers are listed in Table 2.

Bottom Line: The isolates were analyzed by using pulsed-field gel electrophoresis, multilocus sequence typing, and sequencing of the szP gene.Molecular typing methods showed that human and equine isolates were identical or closely related.As leisure and professional equine sports continue to grow, this infection should be recognized as an emerging zoonosis.

View Article: PubMed Central - PubMed

Affiliation: Finnish Food Safety Authority Evira, Kuopio,Finland. sinikka.pelkonen@evira.fi

ABSTRACT
Streptococcus equi subspecies zooepidemicus (S. zooepidemicus) is a zoonotic pathogen for persons in contact with horses. In horses, S. zooepidemicus is an opportunistic pathogen, but human infections associated with S. zooepidemicus are often severe. Within 6 months in 2011, 3 unrelated cases of severe, disseminated S. zooepidemicus infection occurred in men working with horses in eastern Finland. To clarify the pathogen's epidemiology, we describe the clinical features of the infection in 3 patients and compare the S. zooepidemicus isolates from the human cases with S. zooepidemicus isolates from horses. The isolates were analyzed by using pulsed-field gel electrophoresis, multilocus sequence typing, and sequencing of the szP gene. Molecular typing methods showed that human and equine isolates were identical or closely related. These results emphasize that S. zooepidemicus transmitted from horses can lead to severe infections in humans. As leisure and professional equine sports continue to grow, this infection should be recognized as an emerging zoonosis.

Show MeSH
Related in: MedlinePlus