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Genetic variants of Orientia tsutsugamushi in domestic rodents, northern China.

Zhang M, Zhao ZT, Wang XJ, Li Z, Ding L, Ding SJ, Yang HL - Emerging Infect. Dis. (2013)

Bottom Line: We screened Orientia tsutsugamushi from 385 domestic rodents and 19 humans with scrub typhus in rural Tai'an District, Shandong Province, a new scrub typhus epidemic area in northern China.Sequence analysis identified 7 genotypes in the rodents, of which 2 were also identified in the humans.

View Article: PubMed Central - PubMed

ABSTRACT
We screened Orientia tsutsugamushi from 385 domestic rodents and 19 humans with scrub typhus in rural Tai'an District, Shandong Province, a new scrub typhus epidemic area in northern China. Sequence analysis identified 7 genotypes in the rodents, of which 2 were also identified in the humans.

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Phylogenetic relationships of Orientia tsutsugamushi detected in domestic rodents and human patients with scrub typhus in rural areas of Tai’an, Shandong Province, China, September 2010 through March 2012. Relationships were determined on the basis of the partial 56-kDa type-specific antigen gene of O. tsutsugamushi by the minimum-evolution method with the Kimura 2-parameter distance model. Bootstrap values >50% are shown at the branches. Location and GenBank accession numbers are indicated for each sequence. Sampling sources and sampling date are also indicated for sequences determined in the study. Solid triangle indicates sequences determined in this study. Scale bar indicates genetic distance.
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Figure 1: Phylogenetic relationships of Orientia tsutsugamushi detected in domestic rodents and human patients with scrub typhus in rural areas of Tai’an, Shandong Province, China, September 2010 through March 2012. Relationships were determined on the basis of the partial 56-kDa type-specific antigen gene of O. tsutsugamushi by the minimum-evolution method with the Kimura 2-parameter distance model. Bootstrap values >50% are shown at the branches. Location and GenBank accession numbers are indicated for each sequence. Sampling sources and sampling date are also indicated for sequences determined in the study. Solid triangle indicates sequences determined in this study. Scale bar indicates genetic distance.

Mentions: Among the 10 sequences obtained from rodents, we identified 3 genogroups (Kawasaki-related, Fuji-related, and novel genogroup) involving 7 genotypes with >1 nt difference. The genotypes showed 69.7%–99.9% identity with each other. M. musculus and R. norvegicus rodents in Tai’an could serve as reservoirs of 5 and 3 genotypes, respectively. Genotypes KWS1–4 clustered with the Kawasaki genotype (96%–96.5% identity). Genotype FJS was closely related to the Fuji genotype (96.5% identity), which was originally isolated from Leptotrombidium fuji mites in Japan (12). Genotypes SDM1 and SDM2 clustered in an independent clade, which had 70.4%–77.9% identity with reference strains (Figure).


Genetic variants of Orientia tsutsugamushi in domestic rodents, northern China.

Zhang M, Zhao ZT, Wang XJ, Li Z, Ding L, Ding SJ, Yang HL - Emerging Infect. Dis. (2013)

Phylogenetic relationships of Orientia tsutsugamushi detected in domestic rodents and human patients with scrub typhus in rural areas of Tai’an, Shandong Province, China, September 2010 through March 2012. Relationships were determined on the basis of the partial 56-kDa type-specific antigen gene of O. tsutsugamushi by the minimum-evolution method with the Kimura 2-parameter distance model. Bootstrap values >50% are shown at the branches. Location and GenBank accession numbers are indicated for each sequence. Sampling sources and sampling date are also indicated for sequences determined in the study. Solid triangle indicates sequences determined in this study. Scale bar indicates genetic distance.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3713967&req=5

Figure 1: Phylogenetic relationships of Orientia tsutsugamushi detected in domestic rodents and human patients with scrub typhus in rural areas of Tai’an, Shandong Province, China, September 2010 through March 2012. Relationships were determined on the basis of the partial 56-kDa type-specific antigen gene of O. tsutsugamushi by the minimum-evolution method with the Kimura 2-parameter distance model. Bootstrap values >50% are shown at the branches. Location and GenBank accession numbers are indicated for each sequence. Sampling sources and sampling date are also indicated for sequences determined in the study. Solid triangle indicates sequences determined in this study. Scale bar indicates genetic distance.
Mentions: Among the 10 sequences obtained from rodents, we identified 3 genogroups (Kawasaki-related, Fuji-related, and novel genogroup) involving 7 genotypes with >1 nt difference. The genotypes showed 69.7%–99.9% identity with each other. M. musculus and R. norvegicus rodents in Tai’an could serve as reservoirs of 5 and 3 genotypes, respectively. Genotypes KWS1–4 clustered with the Kawasaki genotype (96%–96.5% identity). Genotype FJS was closely related to the Fuji genotype (96.5% identity), which was originally isolated from Leptotrombidium fuji mites in Japan (12). Genotypes SDM1 and SDM2 clustered in an independent clade, which had 70.4%–77.9% identity with reference strains (Figure).

Bottom Line: We screened Orientia tsutsugamushi from 385 domestic rodents and 19 humans with scrub typhus in rural Tai'an District, Shandong Province, a new scrub typhus epidemic area in northern China.Sequence analysis identified 7 genotypes in the rodents, of which 2 were also identified in the humans.

View Article: PubMed Central - PubMed

ABSTRACT
We screened Orientia tsutsugamushi from 385 domestic rodents and 19 humans with scrub typhus in rural Tai'an District, Shandong Province, a new scrub typhus epidemic area in northern China. Sequence analysis identified 7 genotypes in the rodents, of which 2 were also identified in the humans.

Show MeSH
Related in: MedlinePlus