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Human gyrovirus in healthy blood donors, France.

Biagini P, Bédarida S, Touinssi M, Galicher V, de Micco P - Emerging Infect. Dis. (2013)

View Article: PubMed Central - PubMed

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Gyroviruses (GyVs) are naked, single-stranded DNA viruses that were described in chickens in 1979... For >30 years, this virus, which was responsible for severe anemia and increased death rates in young chickens, was considered to have an extremely low genetic diversity and to be specific to this animal host... One study described the detection of GyVs in HIV-positive patients and kidney transplant recipients (0.7% and 6%, respectively), but these viruses had not been identified in blood samples from healthy persons... We investigated the presence of HGyV DNA in 352 blood samples from healthy blood donors in France (mean age 39 years; 185 men; M:F ratio 1:1.11)... The sensitivity of TaqMan assays was estimated to be 10 copies of HGyV DNA by using dilutions of a synthetic template... Each amplification product was subjected to additional agarose gel electrophoresis to help eliminate potential false-negative real-time PCR results... Among the 352 plasma samples tested, 3 (0.85%) resulted in a positive signal by using our in-house real-time detection assay; no positive signal was identified by using the other system tested... When the tests were repeated, identical results were obtained... No intragenetic diversity was identified... Our results demonstrate that recently discovered HGyVs are detectable in blood of healthy persons... A study from Italy of HGyVs in blood from healthy donors did not detect such viruses... The potential clinical importance of HGyVs remains to be clarified... Although infection with CAV in birds is frequently associated with clinical signs and disease, the presence of HGyVs in immunocompromised or immunocompetent humans does not appear to be correlated with visible symptoms... Further studies of the natural history and distribution of HGyVs in human hosts are needed.

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Alignment of partial sequences of human gyroviruses (HGyVs) from healthy blood donors, France. Point mutation corresponding to a nonsynonymous substitution is in boldface (13F1 isolate). Reference sequences and GenBank accession nos.: HGyV1-915, FR823283; HGyV1-CL33, JQ308212; avian gyrovirus (AGV) 2, JQ690763; gyrovirus (GyV) 3, JQ308210). Bar above sequences indicates location of the HGyVsPBp probe; lowercase letters indicate 5′/3′ ends of HGyVsPBs/HGyVsPBr real-time primers; asterisks (*) indicate conserved positions.
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Figure 1: Alignment of partial sequences of human gyroviruses (HGyVs) from healthy blood donors, France. Point mutation corresponding to a nonsynonymous substitution is in boldface (13F1 isolate). Reference sequences and GenBank accession nos.: HGyV1-915, FR823283; HGyV1-CL33, JQ308212; avian gyrovirus (AGV) 2, JQ690763; gyrovirus (GyV) 3, JQ308210). Bar above sequences indicates location of the HGyVsPBp probe; lowercase letters indicate 5′/3′ ends of HGyVsPBs/HGyVsPBr real-time primers; asterisks (*) indicate conserved positions.

Mentions: Plasma samples were prepared as described (6), and 1-mL aliquots were used for nucleic acids extraction (MagNA pure LC; Roche Diagnostics, Meylan, France). HGyV DNA was detected by using 2 systems in separate real-time TaqMan amplification assays (StepOne Plus; Applied Biosystems, Courtaboeuf, France). The first detection assay (VP1 gene) was described previously (HGyV-rtFP/HGyV-rtRP primers, HGyV-rtP probe, 72 nt) (5). The second assay was designed following the analysis of available HGyV sequences (Figure): sense primer HGyVsPBs 5′-GCTAAGACTGTRACATGGC-3′, reverse primer HGyVsPBr 5′-CTCCGGGAATAGCGTCTTC-3′, probe HGyVsPBp 5′-FAM-TGGCACTGGAGACACAGACTGCG-TAMRA-3′. This assay targets the VP2 gene of the viral genome, with an expected length of 118–115 bp, depending on the reference sequence considered.


Human gyrovirus in healthy blood donors, France.

Biagini P, Bédarida S, Touinssi M, Galicher V, de Micco P - Emerging Infect. Dis. (2013)

Alignment of partial sequences of human gyroviruses (HGyVs) from healthy blood donors, France. Point mutation corresponding to a nonsynonymous substitution is in boldface (13F1 isolate). Reference sequences and GenBank accession nos.: HGyV1-915, FR823283; HGyV1-CL33, JQ308212; avian gyrovirus (AGV) 2, JQ690763; gyrovirus (GyV) 3, JQ308210). Bar above sequences indicates location of the HGyVsPBp probe; lowercase letters indicate 5′/3′ ends of HGyVsPBs/HGyVsPBr real-time primers; asterisks (*) indicate conserved positions.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3713844&req=5

Figure 1: Alignment of partial sequences of human gyroviruses (HGyVs) from healthy blood donors, France. Point mutation corresponding to a nonsynonymous substitution is in boldface (13F1 isolate). Reference sequences and GenBank accession nos.: HGyV1-915, FR823283; HGyV1-CL33, JQ308212; avian gyrovirus (AGV) 2, JQ690763; gyrovirus (GyV) 3, JQ308210). Bar above sequences indicates location of the HGyVsPBp probe; lowercase letters indicate 5′/3′ ends of HGyVsPBs/HGyVsPBr real-time primers; asterisks (*) indicate conserved positions.
Mentions: Plasma samples were prepared as described (6), and 1-mL aliquots were used for nucleic acids extraction (MagNA pure LC; Roche Diagnostics, Meylan, France). HGyV DNA was detected by using 2 systems in separate real-time TaqMan amplification assays (StepOne Plus; Applied Biosystems, Courtaboeuf, France). The first detection assay (VP1 gene) was described previously (HGyV-rtFP/HGyV-rtRP primers, HGyV-rtP probe, 72 nt) (5). The second assay was designed following the analysis of available HGyV sequences (Figure): sense primer HGyVsPBs 5′-GCTAAGACTGTRACATGGC-3′, reverse primer HGyVsPBr 5′-CTCCGGGAATAGCGTCTTC-3′, probe HGyVsPBp 5′-FAM-TGGCACTGGAGACACAGACTGCG-TAMRA-3′. This assay targets the VP2 gene of the viral genome, with an expected length of 118–115 bp, depending on the reference sequence considered.

View Article: PubMed Central - PubMed

AUTOMATICALLY GENERATED EXCERPT
Please rate it.

Gyroviruses (GyVs) are naked, single-stranded DNA viruses that were described in chickens in 1979... For >30 years, this virus, which was responsible for severe anemia and increased death rates in young chickens, was considered to have an extremely low genetic diversity and to be specific to this animal host... One study described the detection of GyVs in HIV-positive patients and kidney transplant recipients (0.7% and 6%, respectively), but these viruses had not been identified in blood samples from healthy persons... We investigated the presence of HGyV DNA in 352 blood samples from healthy blood donors in France (mean age 39 years; 185 men; M:F ratio 1:1.11)... The sensitivity of TaqMan assays was estimated to be 10 copies of HGyV DNA by using dilutions of a synthetic template... Each amplification product was subjected to additional agarose gel electrophoresis to help eliminate potential false-negative real-time PCR results... Among the 352 plasma samples tested, 3 (0.85%) resulted in a positive signal by using our in-house real-time detection assay; no positive signal was identified by using the other system tested... When the tests were repeated, identical results were obtained... No intragenetic diversity was identified... Our results demonstrate that recently discovered HGyVs are detectable in blood of healthy persons... A study from Italy of HGyVs in blood from healthy donors did not detect such viruses... The potential clinical importance of HGyVs remains to be clarified... Although infection with CAV in birds is frequently associated with clinical signs and disease, the presence of HGyVs in immunocompromised or immunocompetent humans does not appear to be correlated with visible symptoms... Further studies of the natural history and distribution of HGyVs in human hosts are needed.

Show MeSH